Reagent kit for noninvasive test of cervical cancer susceptibility gene

Abstract

The invention provides a reagent kit for noninvasive test of cervical cancer susceptibility gene, which comprises specific primers, DNA (deoxyribonucleic acid) sequencing primers, PCR(polymerase chain reaction) reaction components, PCR product purifying components, DNA sequencing reaction components and the like, wherein the specific primers and the DNA sequencing primers are used for testing four mononucleotide polymorphism locus genotypes of Arg72Pro(rs1042522) on gene P53, deletion of gene GSTM1 (null / present), deletion of gene GSTT1 gene (null / present) and C677T(rs1801133) on gene MTHFR. The reagent kit can be used evaluating the risk level of female cervical cancer proportion by testing the four mononucleotide polymorphism locus genotypes relative to generation of cervical cancer closely, so that women can be guided to prevent cervical cancer pointedly in terms of the gene according to the gene test result of each woman to be tested, and the proportion of cervical cancer can be reduced. The reagent kit can be used with the oral mucosa cell sampling which is painless and non-invasive, so that cross infection is avoided. In addition, the sequencing test results are accurate and reliable, purchasing of expensive import special instruments is omitted, and the reagent kit is easy to be applied and popularized.

Description

Technical field [0001] The invention belongs to the field of molecular biology, and relates to medicine and biotechnology, and specifically relates to a cervical cancer susceptibility gene detection kit, which evaluates the risk level of cervical cancer from the genetic level according to the test results, and serves as a direction for the prevention and treatment of cervical cancer guide. Background technique [0002] Cervical cancer is one of the common gynecological malignancies. Its incidence ranks second among female malignancies, and its mortality rate gradually rises to the first place. According to statistics from the World Health Organization (WHO), it is estimated that there are about 466,000 new cases of cervical cancer each year, and 80% of these cases occur in developing countries. my country has about 140,000 new cases each year, accounting for 1 / 3 of the total number of new cases of cervical cancer in the world, and the incidence rate is 6 times that of some devel...

AI Technical Summary

Problems solved by technology

MTHFR is the core enzyme of folic acid activation in the process of folic acid metabolism. Studies have found that the C677T polymorphic site mutation on the MTHFR gene can lead to a decrease in the thermostability and enzyme activity of the enzyme, thereby causing abnormal DNA methylation. Abnormal kylation may lead to abnormal expression of oncogenes and tumor suppressor genes, affect the regulation of cell growth and function, and promote the occurrence of cancer

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