Method for detecting rutin and quercetol through ionic liquid-accelerating solvent extraction and high performance liquid chromatograph chemiluminescence

An ionic liquid and accelerated solvent technology, applied in the field of detection of rutin and quercetin, can solve the problems of cumbersome processing of extraction liquid, low extraction efficiency, long extraction time, etc., and achieve improved extraction capacity, high extraction efficiency, and recovery rate. Good results

Inactive Publication Date: 2012-07-04
ZHEJIANG UNIV
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Problems solved by technology

Many kinds of traditional Chinese medicine contain rutin and quercetin. The common extraction methods currently include Soxhlet extraction, ultrasonic-assisted extraction, or microwave-assisted extraction. However, these methods often either take too long or have low extraction efficiency, or It is cumbersome to operate, and the extracted extract needs cumbersome further processing
The commonly used detection method is mainly ultraviolet detection method, which has a high detection limit and is difficult to measure very small components in some drugs.

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  • Method for detecting rutin and quercetol through ionic liquid-accelerating solvent extraction and high performance liquid chromatograph chemiluminescence
  • Method for detecting rutin and quercetol through ionic liquid-accelerating solvent extraction and high performance liquid chromatograph chemiluminescence
  • Method for detecting rutin and quercetol through ionic liquid-accelerating solvent extraction and high performance liquid chromatograph chemiluminescence

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Embodiment Construction

[0014] The technical scheme of the present invention is further described below in conjunction with specific embodiment:

[0015] The specific steps of ionic liquid-accelerated solvent extraction HPLC chemiluminescence detection of rutin quercetin in hawthorn are as follows:

[0016] 1. Preparation of standard solution: Accurately weigh 10 mg of each rutin-quercetin standard sample, place them in two 10ml volumetric flasks, shake to dissolve, and set the volume to the mark;

[0017] 2. Preparation of standard curve: prepare standard solutions with different concentrations by step-by-step dilution;

[0018] 3. Ionic liquid-accelerated solvent extraction: Remove the core of the hawthorn bought in the pharmacy, wash and dry it in the sun, crush it with a pulverizer, pass it through a 40-mesh sieve and collect it, take a certain amount of hawthorn sample and put it into the 34mL extraction pool of the accelerated solvent extraction instrument At the same time, add an appropriate ...

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Abstract

The invention provides a method for extracting and detecting rutin and quercetol, which is easy to realize automation, is environment-friendly, and has accurate and reliable measuring result and high sensitivity. The method adopts environment-friendly ionic liquid to replace poisonous organic solvent, and utilizes the high-temperature high-pressure condition in the accelerating solvent extraction technology to quickly extract active constituents such as rutin and quercetol in hawthorn of the traditional Chinese medicine, the extracted extract only needs to be simply processed and then can be directly transferred to a high performance liquid chromatograph separation system, and the processed extract can be detected by a chemiluminescence detector after being completely separated by a C18 column, the appearance condition is excellent, better detection result can be obtained, and the detection limit is low. The method can be used for detecting rutin and quercetol in foods, health products and other solid samples with very low content of rutin and quercetol.

Description

technical field [0001] The invention provides a method for detecting rutin and quercetin, specifically, a new method for detecting rutin and quercetin by using ionic liquid-accelerated solvent extraction high performance liquid chromatography chemiluminescence. Background technique [0002] Both rutin and quercetin are natural flavonoids, which have strong antioxidant activity, can delay the aging of human cells, and have important physiological and medicinal values ​​for human health. Many kinds of traditional Chinese medicine contain rutin and quercetin. The common extraction methods currently include Soxhlet extraction, ultrasonic-assisted extraction, or microwave-assisted extraction. However, these methods often either take too long or have low extraction efficiency, or It is cumbersome to operate, and the extracted extract needs cumbersome further processing. The commonly used detection method is mainly ultraviolet detection method, which has a high detection limit and...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/08
Inventor 吴宏伟朱岩陈梅兰
Owner ZHEJIANG UNIV
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