Method for extracting pesticide residue of Chinese little greens

Abstract

The invention discloses a method for extracting pesticide residue of Chinese little greens. The method comprises the following steps of: weighing Chinese little greens in a centrifuge tube; adding a mixed extracting solution; ultrasonically extracting; centrifuging for 5min; taking supernate; placing an Envi-18 column on a fixed stand, before adding a sample, firstly, pre-washing the column with the mixed extracting solution, downwards connecting with an inverted conical flask, transferring the supernate; washing the column with the mixed extracting column; concentrating to be 1mL; adding sodium sulfate in an Envi-Carb column, connecting the Envi-Carb column to the top of an aminopropyl column, downwards connecting a serial connection column with the inverted conical flask; before adding the sample, pre-washing the column with the mixed extracting solution and methylbenzene, transferring a concentrated solution to a purification column, washing a sample liquid bottle with the mixed extracting solution and the methylbenzene; adding a liquid storage device on the serial connection column, washing the serial connection column with the mixed extracting solution and the methylbenzene, collecting effluent, concentrating; and adding normal hexane for carrying out solvent exchange, and applying an interior label, wherein the mixed extracting solution comprises 50 percent of acetonitrile, 30 percent of acetone and 20 percent of DMF (Dimethyl Formamide) in percent by volume.

Description

technical field [0001] The invention relates to a method for extracting pesticide residues in vegetables, in particular to a method for extracting pesticide residues in feather vegetables. Background technique [0002] Food safety refers to food that is non-toxic and harmless, meets proper nutritional requirements, and does not cause any acute, subacute or chronic harm to human health. According to the definition of the World Health Organization, food safety is "a public health problem in which toxic and harmful substances in food affect human health". Food safety is also an interdisciplinary field that focuses on ensuring food hygiene and food safety, reducing hidden dangers of disease, and preventing food poisoning in the process of food processing, storage, and sales. [0003] The vegetable food safety problem in our country is very serious, so the supervision and detection of pesticide residues in vegetables becomes very important. At present, the pesticide residues in...

AI Technical Summary

Problems solved by technology

[0004] However, in the prior art, all vegetables, regardless of their species, are extracted with a single acetonitrile solvent, resulting in the disadvantage of low extraction rate, which directly leads to inaccurate detection data of subsequent gas chromatography-mass spectrometer GC-MS. precise