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Rooting transplanting method for tissue culture poplar seedlings

A technique for tissue culture seedlings and poplars, which is applied in the field of rooting and transplanting of poplar tissue culture seedlings, can solve the problems of low survival rate of poplar tissue culture seedlings and the like, and achieves an increase in the survival rate of transplanting, the method is safe and reliable, and the survival rate is increased. rate effect

Inactive Publication Date: 2013-06-19
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The present invention aims to solve the problem that the existing rooting and transplanting method makes the survival rate of poplar tissue culture seedlings low, and provides a rooting and transplanting method for poplar tissue culture seedlings

Method used

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  • Rooting transplanting method for tissue culture poplar seedlings
  • Rooting transplanting method for tissue culture poplar seedlings
  • Rooting transplanting method for tissue culture poplar seedlings

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specific Embodiment approach 1

[0013] Specific embodiment one: the rooting and transplanting method of poplar tissue culture seedling of the present embodiment, carry out according to the following steps: one, poplar tissue culture seedling is put into the test tube that 4~6mL liquid rooting medium is housed, make group Cultivate the seedlings upright, cover the mouth of the test tube with a sealing film, and take root for 11 to 13 days to obtain rooted seedlings; 2. Move the rooted seedlings with a root length of 12 to 14 mm from the test tube to a container with distilled water, and the water depth is 10 to 10 mm. 20mm, buckle the transparent container on the rooted seedlings to keep moisture, hydroponics for 2 days; 3, then transplant the rooted seedlings after hydroponic cultivation into the soil, buckle the transparent container on the rooted seedlings to keep moisture for 16 days, wherein the first 4 days every day Water once, spray once on the inner wall of the transparent container with tap water aft...

specific Embodiment approach 2

[0015] Specific embodiment two: what this embodiment is different from specific embodiment one is: poplar tissue culture seedling described in step one has a terminal bud, 6~8 side leaves, plant height is 6~9cm and stem is thick. 0.6~1.5mm. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0016] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the diameter of the test tube in step 1 is 30 mm and the length is 20 cm. Others are the same as in the first or second embodiment.

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Abstract

A rooting transplanting method for tissue culture poplar seedlings is used for solving the problem that the existing rooting transplanting methods lead to low survival rate of the tissue culture poplar seedlings. The rooting transplanting method for tissue culture poplar seedlings includes: firstly, placing tissue culture poplar seedlings into test tubes containing liquid rooting medium, erectingthe tissue culture poplar seedlings, covering openings of the test tubes with seal films, allowing the seedlings to root to obtain the rooted seedlings; secondly, moving the rooted seedlings with 12-14mm long roots to a container with distilled water from the test tubes, and covering the rooted seedlings with a transparent container to preserve moisture for water culture; and thirdly, transplanting the rooted seedlings subjected to water culture to soil, covering the rooted seedlings with a transparent container to preserve moisture for 16 days, removing the transparent container, transplanting the rooted seedlings with the soil into a greenhouse and completing rooting and transplanting of the tissue culture poplar seedlings. The rooting transplanting method is safe and reliable, and the transplant survival rate of the tissue culture poplar seedlings transplanted into the greenhouse can reach more than 95%.

Description

technical field [0001] The invention relates to a method for rooting and transplanting poplar tissue culture seedlings. Background technique [0002] Plant tissue culture includes a series of processes such as explant collection, medium preparation, filling, disinfection, storage, inoculation, and cultivation. After the tissue culture seedlings are obtained, rooting and transplanting are required. The traditional method uses solid medium for rooting, hardening the seedlings indoors or in soil, and then directly transplanting the rooting and transplanting methods in the greenhouse. After the poplar tissue-cultured seedlings are rooted and cultivated, they are directly transplanted into greenhouses for hardening, which is prone to water loss and wilting, resulting in a low survival rate of the tissue-cultured seedlings. After poplar tissue culture seedlings are cultured by solid rooting, the process of cleaning the root medium is easy to damage the roots and reduce the water ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G31/00A01G1/00A01G13/02
Inventor 刘志华王志英王玉成张荣沭刁桂萍古丽吉米拉.米吉提王娜窦恺
Owner NORTHEAST FORESTRY UNIVERSITY