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Method for improving application effects of liquid enzyme

A liquid enzyme and amylase technology, which is applied in the field of improving the application effect of liquid enzymes, can solve the problems of excessive enzyme dosage, achieve the effects of reducing dosage, improving application effect, and reducing costs

Inactive Publication Date: 2012-07-11
HUNAN LERKAM BIOLOGICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method that can conveniently and efficiently improve the application effect of liquid enzymes in a short period of time, so as to solve the existing technical problems that the amount of enzymes is too large or other chemical auxiliary agents need to be added

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0017] A method for improving the application effect of cellulase. Before using cellulase, add 3% (mass percentage) of heat-resistant protective agent to cellulase, and make cellulase and heat-resistant protective agent mix uniformly, in the optimum temperature of this cellulase 40~80 After heat shock at ℃ for 60 s, cool to room temperature in an ice bath. Wherein, the heat-resistant protective agent is a mixture of 30% glucose, 30% trehalose, 5% pectin, 30% polyethylene glycol and 5% vitamin C. And during the heat shock process of the cellulase, stir and shake constantly to ensure that the cellulase and the heat-resistant protective agent are evenly heated.

Embodiment 2

[0019] A method for improving the application effect of glucoamylase. Before using the glucoamylase, add 6% (mass percentage) heat-resistant protective agent to the glucoamylase, and mix the glucoamylase and heat-resistant protective agent evenly, heat shock at the optimum temperature of the glucoamylase at 50-90°C After 600s, put in cold water to cool to room temperature. Wherein, the heat-resistant protective agent is a mixture of 20% lactose, 30% propolis, 40% carbonyl alcohol and 10% vitamin E. And during the heat shock process of the glucoamylase, constantly stir and shake to ensure that the glucoamylase and the heat-resistant protective agent are evenly heated.

Embodiment 3

[0021] A method for improving the application effect of amylase. Before using the amylase, add 10% (mass percentage) heat-resistant protective agent to the amylase, and mix the amylase and the heat-resistant protective agent evenly, heat shock at the optimum temperature of the amylase at 40-70°C After 300s, put in cold water to cool to room temperature. Wherein, the heat-resistant protective agent is a mixture of 70% mannose, 10% pectin, 19% fatty alcohol polyoxyethylene ether and 1% vitamin B. And during the heat shock process of the amylase, constantly stir and shake to ensure that the amylase and the heat-resistant protective agent are evenly heated.

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Abstract

The invention provides a method for improving application effects of a liquid enzyme. A heat-resisting protective agent is added in the liquid enzyme, heat shock is performed on the liquid enzyme for 3s to 600s at appropriate temperature of the liquid enzyme, and additive amount of the heat-resisting protective agent is 1%-10% of total amount of the liquid enzyme. The heat-resisting protective agent is a mixture of saccharides, adhesives, surface active agents and vitamins, additive amount of the saccharides is 10%-70% of that of the heat-resisting protective agent, additive amount of the adhesives is 5%-30% of that of the heat-resisting protective agent, additive amount of the surface active agents is 5%-40% of that of the heat-resisting protective agent, and additive amount of the vitamins is 1%-20% of that of the heat-resisting protective agent. The appropriate temperature of the liquid enzyme is 30-90 DEG C. The method adopts a hot-shock method to improve the application effects of the liquid enzyme, the heat-resisting protective agent is added in the liquid enzyme before the hot shock, and the application effects can be improved by above 10% under the condition that properties of the liquid enzyme are not changed. For achieving the same application effects, using amount of the liquid enzyme is greatly reduced, and therefore cost is reduced greatly.

Description

technical field [0001] The invention relates to the field of enzyme preparations, in particular to a method for improving the application effect of liquid enzymes. Background technique [0002] At present, generally speaking, the application effect of liquid enzymes is improved through the following three methods: The first method is to improve the application effect by increasing the enzyme activity, usually by ultrafiltration and concentration, but the concentration time is long and the ultrafiltration Membrane consumption is large; the second method is to increase the application effect by increasing the amount of enzyme used, but it increases the cost of packaging, transportation and use; the third method is to compound some additives in the enzyme, including chemical additives and For some other enzymes, since the structure and interaction of various enzymes under these additives are not very clear, there are not many very reasonable formulations. Therefore, it is nece...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/96
Inventor 王文芳李琦宋娟刘绍
Owner HUNAN LERKAM BIOLOGICAL CO LTD
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