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Kit for screening colorectal cancer

A colorectal cancer and kit technology, applied in the biological field, can solve the problems of poor sensitivity, large workload, complex analysis process, etc., and achieve the effects of high sensitivity, convenient sampling and good repeatability

Active Publication Date: 2012-07-11
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Studies have shown that the early diagnosis of colorectal cancer is particularly critical. The five-year survival rate of colorectal cancer patients is as high as 90%, while the current early detection rate of colorectal cancer is less than 40%. Once metastasized, the survival rate of colorectal cancer patients is less than 10%. %
However, some of these methods have complex analysis processes, heavy workload, and poor sensitivity, and cannot efficiently analyze lysophospholipids with low content in body fluids, and none of these methods can be easily used for the separation and analysis of lysophospholipids. Subtype

Method used

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  • Kit for screening colorectal cancer
  • Kit for screening colorectal cancer
  • Kit for screening colorectal cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1, the construction of the kit for screening colorectal cancer

[0045] 1. Establishment of Molecular Model

[0046] 1. Blood collection, lipid molecule extraction and detection

[0047] 1) Research object

[0048] All samples were collected from May 2007 to May 2010. The samples came from the Second Artillery General Hospital of the PLA, Beijing Hospital, and the Second Affiliated Hospital of Peking University. Blood from fasting patients was taken in the morning for pretreatment. This project was approved by the Institutional Review Board, and all subjects signed informed consent.

[0049] Table 1 summarizes the basic situation of the research objects: the proportion of males in the healthy samples and tumor samples were 68% and 59% respectively; the ages of the healthy controls and tumor patients were 55.2±7.5 and 55.7±11.8 respectively, and the sampling age was not significant difference.

[0050] Table 2 summarizes the basic situation of the 120 tum...

Embodiment 2

[0096] Embodiment 2, application of the kit for screening colorectal cancer

[0097] 1. Sensitivity and specificity detection of the kit (model 2) for screening colorectal cancer

[0098] Get the blood of 120 healthy control subjects (all clinically identified) and 120 patients with colorectal cancer (all clinically identified and diagnosed); collect isolated whole blood, fat Extraction method 1 is extracted, liquid mass spectrometer detects 18:1LPC, 18:2LPC, SPC total amount, 14:0LPC, 16:0LPC, 18:0LPC, 20:0LPC and 22:0LPC content;

[0099] Take 11.406×18:2LPC%+7.43×18:1LPC% as the abscissa, take the total amount of saturated LPC as the ordinate, and use the formula of model 2 [11.41×18:2LPC%+7.43×18:1LPC%+0.024×saturated The total amount of LPC=14.58] make a straight line for graphing.

[0100] The result is as figure 2 As shown in the right figure of each colorectal cancer patient (C) and normal control (H), the plasma (11.406×18:2LPC%+7.43×18:1LPC%) is plotted against t...

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Abstract

The invention discloses a kit for screening colorectal cancer, which comprises a device and a comparison card, wherein the device is used for detecting the content of phosphatide; the comparison card is established by utilizing the content of phosphatide; and the phosphatide is SPC (sphingosylphosphorylcholine), 14:0 LPC (lysophosphatidylcholine), 16:0 LPC, 18:2 LPC, 18:1 LPC, 18:0 LPC, 20:4 LPC, 20:0 LPC, 22:6 LPC and 22:0 LPC. A test of the kit proves that an effective early diagnosis molecule mark for the colorectal cancer is developed, a molecule detection model is established, the detection sensitivity and specificity can reach 88% and 80% according to the model, and a distinguishing effect for comparing the colorectal cancer with the normal state obtained by utilizing the lipid molecules is better than the one obtained by using the marks for researching the early diagnosis of the colorectal cancer in recent years.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a kit for screening colorectal cancer. Background technique [0002] In recent years, the global incidence of colorectal cancer has risen sharply. In recent years, more than one million people die of colorectal cancer every year. Because the occurrence of colorectal cancer is closely related to living habits, especially eating habits, the incidence and mortality of colorectal cancer have increased rapidly in my country with the gradual improvement of people's living standards in recent years. Studies have shown that the early diagnosis of colorectal cancer is particularly critical. The five-year survival rate of colorectal cancer patients is as high as 90%, while the current early detection rate of colorectal cancer is less than 40%. Once metastasized, the survival rate of colorectal cancer patients is less than 10%. %. At present, the "gold standard" for diagnosing colorectal canc...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
Inventor 张俊杰李颂宋建稳徐燕桑建利丛羽生
Owner BEIJING NORMAL UNIVERSITY
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