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Method for preparing porous chitosan scaffold

A technology of porous scaffold and chitosan, applied in the field of preparation of chitosan porous scaffold, can solve problems such as poor connectivity, and achieve the effects of improving porosity, simple operation and enhancing connectivity

Inactive Publication Date: 2012-07-18
UNIV OF SHANGHAI FOR SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] One of purpose of the present invention provides a kind of preparation method of chitosan porous scaffold in order to overcome the shortcoming of poor connectivity between pores when preparing tissue engineering porous scaffold by freeze-drying

Method used

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  • Method for preparing porous chitosan scaffold
  • Method for preparing porous chitosan scaffold

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Embodiment 1

[0031] A kind of preparation method of chitosan porous support, comprises the steps:

[0032] (1) Preparation of solution

[0033] Preparation is dissolved with the chitosan acid solution of 0.15g ammonium acetate:

[0034] First measure 1ml of acetic acid and add it to a 100ml volumetric flask to make up the volume to make a 1% (V / V) acetic acid solution;

[0035] Weigh 0.15g of ammonium acetate and add it to 1% (V / V) acetic acid solution, then weigh 3g of chitosan, and add 0.15g of ammonium acetate to the 1% (V / V) acetic acid solution;

[0036] To prepare a 1% (v / v) glutaraldehyde solution:

[0037] Measure 1ml of pure glutaraldehyde and add distilled water to make up to 100ml, store at 4°C for later use;

[0038] (2), Freeze drying

[0039] Weigh 0.2ml of the 1% (v / v) glutaraldehyde solution obtained in step (1), and add it into the chitosan acid solution in which 0.15g of ammonium acetate is dissolved in step (1), after stirring for 15min, put The stirred solution is ...

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Abstract

The invention discloses a method for preparing a porous chitosan scaffold. Chitosan is used as a raw material, and water and ammonium acetate are used as pore foaming agents. The preparation method comprises the following steps of: preparing a chitosan acid solution of the ammonium acetate, injecting the solution into a mold, performing freeze drying, drying the freeze-dried chitosan scaffold for40 minutes in an oven of 95 DEG C, and thus obtaining the porous chitosan scaffold. The aperture of the porous chitosan scaffold obtained by the method for preparing the porous chitosan scaffold is 30 to 60 microns, the porosity is 90 percent, and the community among pores is good. The preparation method is easy to operate, simple in process and high in experimental repetition rate.

Description

technical field [0001] The invention belongs to the field of preparation of porous materials, in particular to a preparation method of a chitosan porous support. Background technique [0002] Natural polymer materials are the earliest medical materials used by humans. They have good biocompatibility, almost all of them are degradable, and the degradation products are non-toxic. Among tissue engineering scaffold materials, typical natural polymer materials include collagen, chitosan, gelatin, and sodium alginate. [0003] Chitosan (chitosan) is obtained by deacetylation of chitin (chitin), which exists widely in nature. The biofunctionality, compatibility, blood compatibility, safety, and microbial degradability of this natural polymer Excellent properties such as chitosan have been widely concerned by all walks of life. In recent years, in the field of cell culture and cell product research, research on chitosan scaffold materials is very extensive. However, its preparatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J9/28C08J9/26C08J9/06C08L5/08C08K5/07C12N5/00
Inventor 李琳刘宝林韩宝三杨波彭承宏
Owner UNIV OF SHANGHAI FOR SCI & TECH
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