Harmless treatment method of coffeine-containing waste water and bacterium used in harmless treatment method

A caffeine and wastewater technology, applied in the biological field, can solve the problems that it is difficult to degrade and utilize caffeine, and there is no caffeine-containing wastewater treatment technology, and achieve a good connection effect

Active Publication Date: 2012-07-25
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because caffeine shows strong resistance to acid-base and common oxidant treatment, it is generally difficult for ordinary microorganisms to degrade and utilize caffeine, and caffeine dissolved in water has good dispersibility and will not affect water bodies like other pollutants. Therefore, even if the wastewater contains a relatively

Method used

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  • Harmless treatment method of coffeine-containing waste water and bacterium used in harmless treatment method
  • Harmless treatment method of coffeine-containing waste water and bacterium used in harmless treatment method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1, purification and domestication of Pseudomonas putida (i.e. caffeine-decomposing bacteria):

[0021] Collect the root topsoil layer from the soil of a tea garden (located in the tea tree germplasm resource nursery of Huajiachi Campus of Zhejiang University) that has been planted for more than 10 years, remove litter and fallen leaves, mix 1 gram of soil with 1 liter of sterile water, and take 0.1 ml of supernatant , inoculated with 20 milliliters of solid bacterial isolation medium (5.0 mmol / L potassium chloride, 1.5 mmol / L ferrous sulfate, 1.5 mmol / L magnesium sulfate, 0.4 mmol / L calcium chloride, 0.3 mmol / L disodium hydrogen phosphate, 0.15 µmol / L boric acid, 0.05 µmol / L copper sulfate, 0.05 µmol / L manganese sulfate, 0.05 µmol / L sodium molybdate, 0.05 micromol / L zinc sulfate, caffeine 25 mmol / L, agar 12 g / L, pH 7.0) in a petri dish; culture upside down at 28-30°C for 3 days, pick white monoclonal bacteria spot, inoculated into 1 ml of liquid purification...

Embodiment 2

[0025] Embodiment 2, caffeine-decomposing bacteria multiply:

[0026] Get 1 liter of Pseudomonas putida (Pseudomonas putida) CGMCC NO.5767 and inoculate it into 250 liters of caffeine-containing expansion liquid medium (5.0 mmol / L potassium chloride, 1.5 mmol / L ferrous sulfate, 1.5 mg mol / L magnesium sulfate, 0.4 mmol / L calcium chloride, 0.3 mmol / L disodium hydrogen phosphate, 0.15 μmol / L boric acid, 0.05 μmol / L copper sulfate, 0.05 μmol / L manganese sulfate, 0.05 μM mol / L sodium molybdate, 0.05 micromol / L zinc sulfate, 10 g / L sucrose, 10 g / L peptone, 7.5 mmol / L caffeine, pH7.0), at 28-30°C, 130-150 rpm Cultivate and multiply under the condition of 10 minutes, when the absorbance of the medium at 600 nanometers is ≥ 4 (the expansion time is about 2-4 days), it can be used for the treatment of caffeine-containing wastewater.

[0027] The preparation method of above-mentioned propagation liquid culture medium is: the potassium chloride of 5.0 millimoles, the ferrous sulfate of 1...

Embodiment 3

[0028] Embodiment 3, a kind of harmless treatment method of caffeine-containing wastewater (i.e. caffeine-decomposing bacteria (Pseudomonas putida) process caffeine-containing wastewater):

[0029] Conventional activated sludge treatment of wastewater generated during conventional production and refining of tea polyphenols was carried out in accordance with "The Combined Process of Heterogeneous Catalytic Oxidation and Cyclic Activated Sludge (CASS) Process for the Treatment of Wastewater from the Refining of Tea Polyphenols" (Zhang Bo, Xiao Qianfen, Xia Siqing . Water Purification Technology, 2011, 30(2): 33-36).

[0030] Centrifuge the multiplied bacterial liquid (obtained from Example 2) at 2000-2500 rpm for 15 minutes to collect the bacterial precipitate as Pseudomonas putida (fresh weight). 0.1 kg of Pseudomonas putida (fresh weight) was inoculated in an aerobic aeration tank (tank) with a volume of 10 tons, and after passing through the wastewater after anaerobic ferment...

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PUM

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Abstract

The invention discloses pseudomonas putida CT2025 with preservation department of CGMCC (China General Microbiological Culture Collection Center) and preservation address of No.3, No.1 Institution, Beichen West Road, Chaoyang District, Beijing, Chinese Academy of Sciences Institute of Microbiology, and preservation date of February 17th, 2012 and preservation number of CGMCC No.5767. The invention also discloses a harmless treatment method of coffeine-containing waste water. The method comprises the following steps of: inoculating 0.8-1.2kg of pseudomonas putida in every 100ton of caffeine-containing waste water, and intervening and applying in any one of the following waste water treatment stages: aerobic treatment stage after anaerobic fermentation and application after the aerobic treatment on the waste water is finished.

Description

technical field [0001] The invention belongs to the field of biotechnology. Specifically, the invention relates to a harmless treatment method for caffeine-containing wastewater by using bacteria to degrade caffeine. Background technique [0002] Tea polyphenols are important physiologically active ingredients extracted from tea leaves. They have many functions such as anti-oxidation, anti-radiation, anti-mutation, anti-tumor, and anti-aging. They have good application prospects in the fields of functional food and medicine. Therefore, tea polyphenols The extraction and purification industry is developing very rapidly. By 2011, there were more than 60 tea polyphenol production enterprises in the country, and there were more than 10 enterprises with a scale of more than 100 tons, with an annual output of more than 4,000 tons. Tea polyphenols are often prepared by extraction, purification, purification and drying processes, among which the purification process is the most imp...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F3/02C12R1/40C02F101/38
CPCY02W10/10
Inventor 陆建良范方媛梁月荣郑新强甘泉董占波
Owner ZHEJIANG UNIV
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