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Breeding method of tetraploid radish

A technology of tetraploid and radish seeds, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low yield and red pigment content of red-skinned and red-hearted radishes

Inactive Publication Date: 2012-08-01
ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to propose a breeding method for tetraploid red-skinned radish to improve the yield and red pigment content of red-skinned red-skinned radish content

Method used

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  • Breeding method of tetraploid radish
  • Breeding method of tetraploid radish
  • Breeding method of tetraploid radish

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] This implementation cultivates tetraploid red-hearted red-skinned radish as follows:

[0020] (1) Preparation of various culture media: including culture media of different culture stages, its components and the weight of each component contained in each liter of culture medium are:

[0021] MS medium, in 1L, consists of: NH 4 HO 3 1650mg, KNO 3 1900mg, CaCl 2 2H 2 O 440mg, KH 2 PO 4 170mg, MgSO 4 ·7H 2 O 370mg, FeSO 4 7H2O 27.8mg, Na2EDTA 37.3mg, H 3 BO 3 6.2 mg, MnSO 4 ·H 2 O 16.9 mg, ZnSO 4 ·7H 2 O 8.6 mg, Na 2 MoO 4 2H 2 O 0.25mg, CuSO 4 ·5H 2 O 0.025mg, CoCl 2 ·6H 2 O 0.025mg, KI 0.83mg, inositol 100mg, glycine 2mg, niacin 0.5mg, vitamin B1 0.1mg, vitamin B6 0.5mg and the rest sterile water, high temperature and high pressure sterilization.

[0022] The germination medium is: 1 L of MS medium + 20 g of sucrose + 8 g of agar, pH 5.8, sterilized under high temperature and high pressure.

[0023] The MS liquid induction medium is: MS med...

Embodiment 2

[0039] The difference between this embodiment and Embodiment 1 is:

[0040] The germination medium is: MS medium 1L + sucrose 20g + agar 9 g, pH5.9;

[0041] MS liquid induction medium is: MS medium 1L+0.15mg NAA+5.5mg 6-BA+100mg colchicine, pH5.9;

[0042] The solid differentiation medium is: MS medium 1L+0.1mg NAA+5.5mg 6-BA+20 g sucrose+9 g agar, pH5.9;

[0043] The rooting medium is: MS medium 1L+0.25 mg NAA+20 g sucrose+8 g agar, pH5.9;

[0044] (2) Cultivate tetraploid red-hearted red-skinned radishes according to the following steps:

[0045] Step 1. Select plump and healthy homozygous diploid red-skinned red-skinned radish seeds (seeds are taken from Zhenjiang Institute of Agricultural Sciences), and disinfect the surface with 75% ethanol for 60 seconds on an ultra-clean workbench; use 1% mercuric chloride (mercuric chloride ) solution for 15 minutes, rinsed with sterile water 5 times;

[0046] Step 2: inoculate the sterile seeds on the MS germination medium, and c...

Embodiment 3

[0054] The difference between this embodiment and Embodiment 1 is:

[0055] The germination medium is: MS medium 1L + sucrose 20g + agar 8.5 g, pH 6.0;

[0056] MS liquid induction medium is: MS medium 1L+0.2mg NAA+6mg 6-BA+200mg colchicine, pH6.0;

[0057] The solid differentiation medium is: MS medium 1L+0.15mg NAA+6.0mg 6-BA+20 g sucrose+9 g agar, pH6.0;

[0058] The rooting medium is: MS medium 1L+0.3 mg NAA+20 g sucrose+9g agar, pH6.0;

[0059] (2) Cultivate tetraploid red-hearted red-skinned radishes according to the following steps:

[0060] Step 1. Select plump and healthy homozygous diploid red-skinned red-skinned radish seeds (seeds are from Zhenjiang Institute of Agricultural Sciences), and disinfect the surface with 73% ethanol on the ultra-clean workbench for 40 seconds; use 1% mercuric chloride (mercuric chloride ) solution for 13 minutes, rinsed with sterile water 4 times;

[0061] Step 2. Inoculate the sterile seeds on the MS germination medium, place them ...

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Abstract

The invention relates to a breeding method of a radish, in particular to a breeding method of a tetraploid red-heart red-skin radish, which belongs to the technical field of plant breeding. The method mainly comprises inoculating seeds of the red-heart red-skin radish after sterilization on an MS sprouting culture medium to sprout, placing sterile seedlings in an MS liquid induction culture medium containing colchicine so that the sterile seedlings are cultured, then placing the sterile seedling in a solid differentiation culture medium not containing colchicine so that the sterile seedlings are cultured, when the regeneration buds are generated by differentiation, chopping and inoculating the regeneration buds to a rooting culture medium so as to achieve rooting culture, and obtaining regeneration plants by seedling exercising and transplantation. By detection, the obtained tetraploid red-heart red-skin radish has the advantages over an auto-diploid in each period of plant growth. The breeding method of the tetraploid red-heart red-skin radish has the advantages of improving content of haematochrome of the red-heart red-skin radish while increasing yield of the red-heart red-skin radish, and having broad market prospect.

Description

technical field [0001] The invention relates to a breeding method of radish, in particular to a breeding method of tetraploid red-hearted red-skinned radish, belonging to the technical field of plant breeding. Background technique [0002] "Red heart radish, also known as rouge radish, is famous for its red skin and crisp texture, and is widely cultivated in Guizhou, Sichuan, and Chongqing" (see "Chinese Radish" published by Science and Technology Literature Publishing House, p.172). Red-skinned red-heart radish is a local variety of the genus Radish in the family Brassicaceae. [0003] It is mainly produced in Fuling District, Chongqing City, and it is also cultivated in neighboring counties and cities, Sichuan, Guizhou and other provinces. Its fleshy root bark and root meat are all red, rich in pigment content, and its edible part has a tight texture and rough texture, which is not suitable for raw food but can be used for pickling pickles. In recent years, red-skinned a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张振超潘跃平戴忠良秦文斌姚悦梅潘永飞肖燕
Owner ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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