Preparation method and application of compound cimicifugamide

A technology of cimicamide and compound, which is applied in the field of preparation of glycoside compound cimicamide, can solve the problems of difficult development, difficulty in obtaining standard samples, and few researches on glycoside-bound aroma components, and achieves good effect and low volatility Effect

Inactive Publication Date: 2012-08-01
CHINA TOBACCO SICHUAN IND CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are not many studies on the glycoside-bound flavor components in tobacco. This may be that on the one hand, the influence of glycoside flavor precursors in tobacco on the taste of tobacco has not been paid attention to. On the other hand, glycoside compounds are secondary metabolites of

Method used

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  • Preparation method and application of compound cimicifugamide
  • Preparation method and application of compound cimicifugamide
  • Preparation method and application of compound cimicifugamide

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0032] Example 1:

[0033] Take 20 kg of the cured tobacco leaves and extract them three times with 95% ethanol at a temperature of 60℃. The amount of 95% ethanol is 12, 10, and 8 times the weight of the tobacco leaves. The extraction time is 180, 120, and 90 minutes. The extract was concentrated under reduced pressure and dissolved in water, and then extracted with petroleum ether, ethyl acetate and n-butanol in sequence, and the extract was concentrated under reduced pressure. After the n-butanol extract is crudely separated by resin, it is separated by 300 mesh silica gel and C-18 reversed-phase silica gel column chromatography to obtain 25 mg of cimicamide.

Example Embodiment

[0034] Example 2:

[0035] Take 20 kg of the cured tobacco leaves and extract them three times with methanol at a temperature of 50°C. The amount of methanol is 12, 10, and 8 times the weight of the tobacco leaves. The extraction time is 120, 90, and 60 minutes. After pressure concentration, add water to dissolve, and then extract with petroleum ether, ethyl acetate and n-butanol, and concentrate the extract under reduced pressure. After the n-butanol extract is crudely separated by resin, it is separated by 150 mesh silica gel and C-18 reversed-phase silica gel column chromatography to obtain 27 mg of cimamide.

Example Embodiment

[0036] Example 3:

[0037] Take 20 kg of the cured tobacco leaves and extract them three times with n-butanol at a temperature of 70℃. The amount of n-butanol is 12, 10, and 8 times the weight of the tobacco leaves. The extraction time is 120, 90, and 60 minutes. The extract was concentrated under reduced pressure and dissolved in water, and then extracted with petroleum ether, ethyl acetate and n-butanol. The extract was concentrated under reduced pressure. After the n-butanol extract is crudely separated by resin, it is separated by 60 mesh silica gel and C-18 reversed-phase silica gel column chromatography to obtain 21 mg of cimamide.

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Abstract

The invention provides a preparation method for a compound I and the application of the compound I as a tobacco flavor modifier. The method comprises the following steps of: extracting cured tobacco leaves which serve as a raw material by using water, an organic solvent or a mixed solvent consisting of the water and the organic solvent, concentrating extract under reduced pressure, dissolving concentrate in the water, performing extraction sequentially by using petroleum ether, ethyl acetate and N-butanol, concentrating extract under reduced pressure, and chromatographically separating N-butanol extract by using a macroporous adsorption resin, silica gel and a C-18 silica gel column to obtain the compound. When the compound is added into tobacco shreds, tobacco leaves, expanded tobacco shreds and flaky tobacco shreds, the tobacco flavor can be remarkably improved, smoke can be rounded, remaining taste can be improved, and the inner quality of the tobacco can be improved. The compound I is extracted from the tobacco for the first time, is low in volatility, is flavorless at normal temperature, releases flavor components only after glucosidic bonds of the compound are split when a cigarette is lighted, and has the characteristic of flavor releasing persistence.

Description

technical field [0001] The invention relates to a preparation method and application of a tobacco aroma improving agent, in particular to a preparation method and application of a glycoside compound cimicamide isolated from tobacco leaves and having improved tobacco aroma. Background technique [0002] With the upsurge of the "anti-smoking" movement, smoking and health have attracted more and more attention from the society, and people's demand for low-tar and low-harm cigarettes has become increasingly urgent. Cigarette taste is an important manifestation of tobacco quality, but it will be accompanied by the loss of cigarette flavor while reducing tar. With the in-depth advancement of harm reduction and tar reduction, the tobacco industry has increasingly prominent requirements for aroma compensation brought about by harm reduction and tar reduction. Therefore, finding additives to enhance cigarette flavor to compensate for the flavor loss of low-tar cigarettes is an urgent...

Claims

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Application Information

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IPC IPC(8): C07H15/203C07H1/08A24B3/12
Inventor 周容李东亮冯广林李国友杨杰汪显军
Owner CHINA TOBACCO SICHUAN IND CO LTD
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