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Method for separating adenosine cyclophosphate

A technology for cyclic adenosine monophosphate and metal ions, which is applied in the field of separation of cyclic adenosine monophosphate with new adsorbents, can solve the problems of low adsorption capacity and large consumption of adsorbents, and achieve the effect of low price

Active Publication Date: 2014-04-02
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a method for separating cyclic adenosine monophosphate using a chelating adsorption medium, which effectively solves the problems of low adsorption capacity of the adsorbent, elution and regeneration process that consume a large amount of acid, Alkaline, water problem

Method used

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  • Method for separating adenosine cyclophosphate
  • Method for separating adenosine cyclophosphate
  • Method for separating adenosine cyclophosphate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034]Weigh 50g of cation exchange resin Amberlite IR-124 and pack it into a column, and flow the ferric chloride solution with a concentration of 3g / L through the resin column at 1BV / h, and stop the transformation when the concentration of the effluent is also 3g / L. Continue to feed deionized water at the same flow rate until the effluent does not contain iron ions. Finally, the resin column was rinsed with ethanol. After the rinse was completed, the resin was vacuum-dried in a vacuum oven at 30°C to complete the transformation of the adsorbent. Take 20g of transformed adsorbent to fill the resin column, after equilibrium, put the cyclic adenosine monophosphate solution with a concentration of 4.5g / L on the column, flow through the resin column at 3.5BV / h, and measure the adsorption capacity to be 126mg / g wet after adsorption saturation. resin. Carry out elution with 0.4mol / L sodium hydroxide, elution flow rate 1BV / h, eluent dosage is 3BV, collects eluent, the concentration ...

Embodiment 2

[0036] Weigh 50g of cation exchange resin Amberlite IR-116 and pack it into a column, flow the copper chloride solution with a concentration of 10g / L through the resin column at 1BV / h, and stop the transition when the concentration of the effluent is also 10g / L. Continue to feed deionized water at the same flow rate until the effluent does not contain copper ions. Finally, the resin column was rinsed with ethanol. After the rinse was completed, the resin was vacuum-dried in a vacuum oven at 40°C to complete the transformation of the adsorbent. Take 10g of transformed adsorbent to fill the resin column, equilibrate and put the cyclic adenosine monophosphate solution with a concentration of 4g / L on the column, flow through the resin column at 2BV / h, and measure the adsorption capacity to be 85mg / g wet resin after adsorption saturation. Then use 0.3mol / L sodium hydroxide for elution, the elution flow rate is 2BV / h, and the amount of eluent is 4BV. The eluate was collected, the c...

Embodiment 3

[0038] Weigh 50g of cation exchange resin Amberlite IR-116 to pack into the column, and flow the ferrous chloride solution with a concentration of 15g / L through the resin column at 1BV / h, and stop the transition when the concentration of the effluent is also 15g / L. Continue to feed deionized water at the same flow rate until the effluent does not contain ferrous ions. Finally, the resin column was rinsed with ethanol. After the rinse was completed, the resin was vacuum-dried in a vacuum oven at 50°C to complete the transformation of the adsorbent. Take 30g transformed adsorbent to fill the resin column, after equilibrium, put the cyclic adenosine monophosphate solution with a concentration of 3g / L on the column, flow through the resin column at 3.5BV / h, and measure the adsorption capacity after adsorption saturation is 109mg / g wet resin . Use 0.2mol / L sodium hydroxide for elution, the elution flow rate is 2.5BV / h, and the amount of eluent is 5BV. The eluate was collected, th...

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Abstract

The invention discloses a method for separating adenosine cyclophosphate. The method comprises the following steps of: adsorbing adenosine cyclophosphate in a solution by taking a novel chelation adsorption medium as an adsorbent; eluting the adsorbent by taking an alkaline liquor as an eluent; collecting an eluent; and regenerating the adsorbent by taking an acid liquor as a regenerating agent. In the method provided by the invention, the novel chelation adsorption medium is used for separating adenosine cyclophosphate. Compared with the conventional method for separating adenosine cyclophosphate with resin, the method has the advantages that: the adsorption medium can be suitable for an adenosine cyclophosphate system under any condition that the pH is 1-7, the adsorption amount can be 80-160 mg / g that of resin, and the concentration of adenosine cyclophosphate can be increased by 4-5 times. Moreover, in eluting and regenerating processes in which the novel medium is used, the using amounts of acid and alkali can be saved by over 50 percent, and the using amount of water can be saved by 20-30 percent. The adsorbent is a clean, environmentally-friendly, low-price and readily-available adsorbent.

Description

technical field [0001] The invention belongs to the field of biological separation, and in particular relates to a method for separating cyclic adenosine monophosphate by using a novel adsorbent. Background technique [0002] Cyclic adenosine monophosphate, also known as 3', 5'-cyclic adenosine monophosphate, referred to as cAMP, is an important physiologically active substance in organisms. It has the function of regulating various enzyme-catalyzed reactions in organisms. It is the second messenger. Its main physiological functions are: promoting myocardial contraction, relaxing smooth muscle, expanding coronary blood vessels, improving liver function, promoting nerve regeneration, inhibiting the division of epithelial cells in the outer layer of the skin and transforming abnormal cells, promoting the activity of respiratory chain oxidase and improving Myocardial hypoxia, etc., play an important role in the normal metabolism of the human body. [0003] There are three mai...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H19/213C07H1/06
Inventor 应汉杰杨丽钱文斌吴菁岚柏建新陈勇陈晓春谢婧婧
Owner NANJING TECH UNIV