Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing multiple oligosaccharides by separating and purifying Chinese dates

A technology for separation and purification of oligosaccharides, applied in the fields of oligosaccharides, chemical instruments and methods, preparation of sugar derivatives, etc., can solve the problems of difficult development and utilization, unfavorable functional research, etc., and achieves strong practicability and simple operation process. Effect

Inactive Publication Date: 2012-08-01
SHANXI UNIV
View PDF2 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The jujube oligosaccharide product obtained by the above method still belongs to a mixed product, which is not conducive to further functional research on it, so it is difficult to further develop and utilize it in the field of functional food and medicine

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing multiple oligosaccharides by separating and purifying Chinese dates
  • Method for preparing multiple oligosaccharides by separating and purifying Chinese dates
  • Method for preparing multiple oligosaccharides by separating and purifying Chinese dates

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Remove the core of 100 grams of common jujube after cleaning, add 2000 grams of water, extract for 9 hours at 85° C., filter through gauze to obtain the extract, and vacuum filter the extract to remove suspended and particulate matter; the filtrate uses molecular weight cut-off Ultrafiltration is performed on a 10,000 membrane at 0.12MPa and a filtrate concentration of 3% to remove proteins and polysaccharides, and the permeate is collected; the permeate is then nanofiltered with a membrane with a molecular weight cut-off of 500 at 0.54MPa and a filtrate concentration of 1% , to remove substances such as monosaccharides, collect the retentate, and obtain jujube crude oligosaccharides (JuBO), with a yield of 17.9 ± 0.6%, and a purity of 58.6 ± 0.5%; JuBO is decolorized with a strong basic anion exchange resin, and the static adsorption decolorization condition For: temperature 40°C, resin dosage 7ml / 50ml, concentration 2%, decolorization time 3h; decolorized Ju...

Embodiment 2

[0033] Example 2: JuBO is decolorized with a strong basic anion exchange resin, and the dynamic adsorption process is as follows: sample loading 4BV, sample concentration 3%, elution flow rate 1.5BV / h, eluent distilled water consumption 2BV; other steps are the same as the examples 1. In this example, the type and monosaccharide composition of oligosaccharides obtained by separation and purification are the same as those in Example 1.

Embodiment 3

[0034] Embodiment 3: the raw material that adopts is defective jujube, and all the other steps are with embodiment 1. The yield of crude oligosaccharides obtained after membrane separation was 16.4±0.8%, and the purity was 56.3±0.4%. In this example, the oligosaccharide species and oligosaccharide monosaccharide composition obtained by separation and purification are the same as in Example 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for preparing multiple oligosaccharides by separating and purifying Chinese dates. The method comprises the following steps of extracting by a water extraction method, separating by a membrane separation technology, decoloring a strong-base anion exchange resin, and purifying by a DEAE-cellulose (Diethylaminoethyl Cellulose) column and a dextrangel column to obtain four oligosaccharides with edible Chinese dates or imperfect Chinese dates as raw materials. The invention provides a new direction for development of deep processing products of Chinese dates and provides new resources for development of the oligosaccharides. The method, provided by the invention, has the advantages of simple operation process and is suitable for industrial production. The prepared oligosaccharides have higher purity and can be used for functional research and development of function food and medicines.

Description

1. Technical field [0001] The invention relates to extraction and separation technology of effective components of plant fruits, in particular to a method for separating and purifying various oligosaccharides from jujube. 2. Background technology [0002] Jujube is the fruit of Ziziphus jujuba Mil1, a plant belonging to the family Rhamnaceae. It is native to the Yellow River Basin in China. It has a history of artificial cultivation in my country for 5,000 years. It has extremely high nutritional value and good medical treatment. Health effects. However, with the expansion of cultivation area in recent years, due to the change of cultivation ecology and the increase of susceptible hosts, the diseases of jujube have been increasing year by year. In particular, the problems of defective, inferior, cracked and diseased jujubes have become more and more prominent, seriously affecting the development of the jujube industry. Processing and utilization of jujube, especially defect...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C08B37/00C07H1/08C07H3/06
Inventor 林勤保原超范三红赵子青
Owner SHANXI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com