Kit for detecting concentration of docetaxel in blood

A technology of docetaxel and diagnostic kits, which is applied in the field of diagnostic kits for detecting the concentration of docetaxel in blood, can solve the problems of low-cost throughput and incapable of large-scale application, and achieve simple operation and low cost Effect

Active Publication Date: 2012-08-01
AILEX TECH GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, HPLC and chromatographic-mass spectrometry cannot be applied to clinical practice on a large scale under the existing medical system, and maintain low cost and high throughput.

Method used

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  • Kit for detecting concentration of docetaxel in blood
  • Kit for detecting concentration of docetaxel in blood
  • Kit for detecting concentration of docetaxel in blood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Reagent R1:

[0046]

[0047] The rest is deionized water, and the pH value of reagent R1 is 7.0.

[0048] Reagent R2 is prepared by chemical cross-linking method, including the activation of nanospheres and the combination of docetaxel antibody and nanospheres. The specific steps are as follows:

[0049] (1) Activation of nanospheres

[0050] A) Add 50ug of human anti-docetaxel antibody to 2400ul of buffer solution with a pH value of 7.40, mix well and set aside.

[0051] B) 50 mg of nano-microspheres with a diameter of 100 nm were taken, centrifuged at 4° C., 20,000 rpm, for 20 min, and the filtrate was discarded.

[0052] C) Add 2000 ul of phosphate buffer solution with a concentration of 10 mM, pH 7.40, dissolve the particles with an ultrasonic cleaning agent, and wash by centrifugation for three times.

[0053] D) Add a buffer solution with a pH value of 7.40, sonicate, so that the activation concentration of the nanospheres is 2.5 mg / ml; add 20 mg of N-hydro...

Embodiment 2

[0069] Reagent R1:

[0070]

[0071] The rest is deionized water, and the pH value of reagent R1 is 7.0.

[0072] Reagent R2 is prepared by chemical cross-linking method, including the activation of nanospheres and the combination of docetaxel antibody and nanospheres. The specific steps are as follows:

[0073] (1) Activation of nanospheres

[0074] A) Add 60ug of docetaxel antibody to 2400ul of pH7.40 buffer, mix well and set aside.

[0075] B) Take 40 mg of nano-microspheres with a diameter of 120 nm, centrifuge at 4° C., 20,000 rpm, for 20 min, and discard the filtrate.

[0076] C) Add 2000 ul of phosphate buffer solution with a concentration of 10 mM, pH 7.40, dissolve the particles with an ultrasonic cleaning agent, and wash by centrifugation for three times.

[0077] D) Add a buffer solution with a pH value of 7.40, sonicate, so that the latex particle activation concentration is 2.5 mg / ml; add 25 mg of N-hydroxysuccinimide and 12 mg of l-(3-dimethylaminopropyl)-3...

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Abstract

The invention relates to an assay kit and in particular relates to an assay kit for detecting the concentration of docetaxel in blood. The kit comprises a reagent R1, a nano-microspherical reagent R2 combined with an anti-human docetaxel antibody, and a docetaxe standard product. Compared with the prior art, the assay kit has technical parameters similar to a high performance liquid chromatography method for detecting the docetaxel concentration, such as precision, accuracy, limit of detection and the like, can be widely applied to various hospitals with full-automatic biochemical analyzers relative to the high performance liquid chromatogrpahy method and chromatography-mass chromatography, and has the advantages of convenience in operation and low cost, so that detection of the concentration of docetaxel in massive blood samples can be fulfilled, and the requirement of clinical real-time detection of the concentration of docetaxel in cancer patient blood can be met.

Description

[technical field] [0001] The invention relates to a diagnostic kit, in particular to a diagnostic kit for detecting the concentration of docetaxel in blood. [Background technique] [0002] Docetaxel (Taxotere) is a new type of antineoplastic drug, which belongs to taxane compounds and is a white or nearly white powder. Its mechanism of action is to strengthen tubulin polymerization and inhibit microscopic depolymerization , leading to the formation of stable non-functional microtubule bundles, thereby disrupting mitosis of tumor cells. In recent years, docetaxel has been widely used in the clinical treatment of various tumors. The molecular structure of docetaxel is as follows: [0003] [0004] Docetaxel can be applied to breast cancer. Docetaxel is a new generation of semi-synthetic taxane anticancer drug. The results of clinical trials have shown that adjuvant chemotherapy based on docetaxel can improve the long-term survival rate of early breast cancer. survival an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
Inventor 李子樵郭易萍
Owner AILEX TECH GRP CO LTD
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