Full-solid urase electrode and preparation method thereof

An all-solid-state, urease technology, applied in the field of electroanalytical chemical detection, can solve the problems of long time, high cost, need to add reagents, etc., and achieve the effects of fast response, low production cost, and simple production.

Inactive Publication Date: 2012-08-08

NANJING UNIV OF TECH

2 Cites 7 Cited by

AI-Extracted Technical Summary

Problems solved by technology

[0004] At present, spectrophotometry is mostly used in the determination of urea, which takes a long time and needs to add reagents, and is not suitable for the determination of urea in colored samples and biological fluids. Although...

Abstract

The invention discloses a full-solid urase electrode and a preparation method thereof and belongs to the technical field of electroanalytical chemistry detection. The urase electrode is prepared through the following steps: printing metal oxide and urase onto a substrate by utilizing a screen printing technology; and forming a layer of uniform complex film on the surface of an electrode. The urase electrode prepared by the invention can be directly applied to the rapid detection on the concetration of urea, is good in catalytic performance and is high in stability. The urase electrode is small and portable, rapid in response, simple in manufacturing process, low in manufacturing cost, suitable for volume production and especially suitable for real-time detection such as field rescue, clinical examination and environmental monitoring.

Application Domain

Material analysis by electric/magnetic means

Technology Topic

ChemistryUrea +7

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Examples

Experimental program(3)

Example Embodiment

[0039] Example 1

[0040] The preparation process and steps in this embodiment are as follows:

[0041] (1) A layer of silver paste is printed on the polyvinyl chloride vinyl board by a screen printing method. The silver paste layer is divided into the first contact electrode 2-1, the second contact electrode 2-2, the conductive layer 9, and the first reaction zone 10. -1 and the second reaction zone 10-2;

[0042] (2) Preparation of reference electrode: prepare bovine serum albumin into an aqueous solution with a mass concentration of 5%; add nano-MnO 2 Disperse in the carbon slurry to form a mixture, nano-MnO 2 The mass concentration in the carbon paste is 30%; a 12% bovine serum albumin solution by weight of the mixture is added to the mixture to prepare the second response layer slurry, and the response layer slurry is printed by screen printing. In the second reaction zone 10-2, the second response layer 4-2 is printed to completely cover the second reaction zone 10-2 and become the reference electrode. The second response layer completely covers the silver paste layer, and the temperature is 0℃~4℃. Dry and become a reference electrode;

[0043] (3) Preparation of working electrode: prepare bovine serum albumin into an aqueous solution with a mass concentration of 5%, and urease into an aqueous solution with a mass concentration of 10%; mix nano-MnO 2 Disperse in the carbon slurry to form a mixture, nano-MnO 2 The mass concentration in the carbon slurry is 30%; 15% urease solution and 12% bovine serum albumin solution are added to the mixture by weight to prepare a response slurry, which is printed by screen printing , The first response layer 4-1 is printed on the first reaction zone 10-1 to completely cover the first reaction zone 10-1, and dried at 0°C-4°C to become a working electrode.

[0044] (4) Printed insulating layer: using screen printing method, on the reference electrode, the insulating film layer 3 is printed around the second response layer 4-2, and the insulating film layer 3 vacates the second response layer 4-2 area ; On the working electrode, the insulating film layer 3 is printed around the first response layer 4-1, and the insulating film layer 3 vacates the area of the first response layer 4-1; the two vacant areas on the insulating layer are about 4mm in diameter. The first response layer 4-1 and the second response layer 4-2 are in contact with the solution to be measured during the measurement process through this opening.

[0045] (5) Stick the diaphragm layer on the insulating layer. The diaphragm layer 5 vacates the second response layer 4-2 area and the first response layer 4-1 area, and presses the salt bridge 7 on the diaphragm layer 5, and the salt bridge 7 The two ends are located in the cavity 6-1 and the cavity 6-2, the cover plate 8 is covered, and the salt bridge is located under the cover plate vent 8-3.

[0046] The method and process for measuring the concentration of urea solution by the urease sensor are as follows:

[0047] Add the urea sample to the remaining sample port 8-1, and add the pH standard solution to the sample port 8-2. The contact electrode contacts the contact end of a conventional measuring instrument to measure the potential difference between the working electrode and the reference electrode.

[0048] Figure 4 In order to use the all-solid urease electrode prepared in this implementation to measure the potential response standard curve obtained by different concentrations of urea solution, the standard equation is ΔE(mV)=20.743c(mmol/L)-4.971. As shown by the straight line in the figure, ΔE represents the response electromotive force, c represents the concentration of urea in the solution, and the correlation coefficient R2 is 0.9918.

Example Embodiment

[0049] Example 2

[0050] The preparation process and steps in this embodiment are as follows:

[0051] (1) A layer of silver paste is printed on the polyvinyl chloride vinyl board by a screen printing method. The silver paste layer is divided into the first contact electrode 2-1, the second contact electrode 2-2, the conductive layer 9, and the first reaction zone 10. -1 and the second reaction zone 10-2;

[0052] (2) Preparation of reference electrode: prepare bovine serum albumin into an aqueous solution with a mass concentration of 5%; add nano-SnO 2 Disperse in carbon paste to form a mixture, nano-SnO 2 The mass concentration in the carbon paste is 20%; a bovine serum albumin solution of 7% by weight of the mixture is added to the mixture to prepare the second response layer slurry, and the response layer slurry is printed by screen printing. In the second reaction zone 10-2, the second response layer 4-2 is printed to completely cover the second reaction zone 10-2 and become the reference electrode. The second response layer completely covers the silver paste layer, and the temperature is 0℃~4℃. Dry and become a reference electrode;

[0053] (3) Preparation of working electrode: prepare bovine serum albumin into an aqueous solution with a mass concentration of 5%, and urease into an aqueous solution with a mass concentration of 10%; add nano-SnO 2 Disperse in carbon paste to form a mixture, nano-SnO 2 The mass concentration in the carbon slurry is 20%; add 10% urease solution and 7% bovine serum albumin solution by weight of the mixture to the mixture to prepare a response slurry, and print the response layer slurry by screen printing , The first response layer 4-1 is printed on the first reaction zone 10-1 to completely cover the first reaction zone 10-1, and dried at 0°C-4°C to become a working electrode.

[0054] (4) Printed insulating layer: using screen printing method, on the reference electrode, the insulating film layer 3 is printed around the second response layer 4-2, and the insulating film layer 3 vacates the second response layer 4-2 area ; On the working electrode, the insulating film layer 3 is printed around the first response layer 4-1, and the insulating film layer 3 vacates the area of the first response layer 4-1; the two vacant areas on the insulating layer are about 4mm in diameter. The first response layer 4-1 and the second response layer 4-2 are in contact with the solution to be measured during the measurement process through this opening.

[0055] (5) Stick the diaphragm layer on the insulating layer. The diaphragm layer 5 vacates the second response layer 4-2 area and the first response layer 4-1 area, and presses the salt bridge 7 on the diaphragm layer 5, and the salt bridge 7 The two ends are located in the cavity 6-1 and the cavity 6-2, the cover plate 8 is covered, and the salt bridge is located under the cover plate vent 8-3.

[0056] The method and process of measuring the concentration of urea solution by the urease sensor are as follows:

[0057] Add a sample of urea into the remaining sample port 8-1, and add a pH standard solution to the sample port 8-2. The contact electrode contacts the contact end of a conventional measuring instrument to measure the potential difference between the working electrode and the reference electrode.

[0058] Figure 5 In order to use the all-solid urease electrode prepared in this implementation to measure the potential response standard curve obtained by different concentrations of urea solution, the standard equation is ΔE(mV)=21.486c(mmol/L)-10.900. As shown by the straight line in the figure, ΔE represents the response electromotive force, c represents the concentration of urea in the solution, and the correlation coefficient R2 is 0.9927.

Example Embodiment

[0059] Example 3

[0060] The preparation process and steps in this embodiment are as follows:

[0061] (1) A layer of silver paste is printed on the polyvinyl chloride vinyl board by a screen printing method. The silver paste layer is divided into the first contact electrode 2-1, the second contact electrode 2-2, the conductive layer 9, and the first reaction zone 10. -1 and the second reaction zone 10-2;

[0062] (2) Preparation of reference electrode: prepare bovine serum albumin into an aqueous solution with a mass concentration of 5%; add nano-RuO 2 Disperse in the carbon paste to form a mixture, nano-RuO 2 The mass concentration in the carbon paste is 10%; a 2% bovine serum albumin solution by weight of the mixture is added to the mixture to prepare the second response layer slurry, and the response layer slurry is printed by screen printing. In the second reaction zone 10-2, the second response layer 4-2 is printed to completely cover the second reaction zone 10-2 and become the reference electrode. The second response layer completely covers the silver paste layer, and the temperature is 0℃~4℃. Dry and become a reference electrode;

[0063] (3) Preparation of working electrode: prepare bovine serum albumin into an aqueous solution with a mass concentration of 5%, and urease into an aqueous solution with a mass concentration of 10%; add nano-RuO 2 Disperse in the carbon slurry to form a mixture, nano-RuO 2 The mass concentration in the carbon slurry is 10%; 5% by weight of the mixture of urease solution and 2% bovine serum albumin solution are added to the mixture to prepare a response slurry, and the response layer slurry is printed by screen printing , The first response layer 4-1 is printed on the first reaction zone 10-1 to completely cover the first reaction zone 10-1, and dried at 0°C-4°C to become a working electrode.

[0064] (4) Printed insulating layer: using screen printing method, on the reference electrode, the insulating film layer 3 is printed around the second response layer 4-2, and the insulating film layer 3 vacates the second response layer 4-2 area ; On the working electrode, the insulating film layer 3 is printed around the first response layer 4-1, and the insulating film layer 3 vacates the area of the first response layer 4-1; the two vacant areas on the insulating layer are about 4mm in diameter. The first response layer 4-1 and the second response layer 4-2 are in contact with the solution to be measured during the measurement process through this opening.

[0065] (5) Stick the diaphragm layer on the insulating layer. The diaphragm layer 5 vacates the reaction area of the electrode, and the diaphragm layer 5 vacates the second response layer 4-2 area and the first response layer 4-1 area, and bridges the salt 7 is pressed on the diaphragm layer 5, the two ends of the salt bridge 7 are located in the cavity 6-1 and the cavity 6-2, the cover plate 8 is covered, and the salt bridge is located under the cover plate vent 8-3.

[0066] The method and process for measuring the concentration of urea solution by the urease sensor are as follows:

[0067] Add a sample of urea into the remaining sample port 8-1, and add a pH standard solution to the sample port 8-2. The contact electrode contacts the contact end of a conventional measuring instrument to measure the potential difference between the working electrode and the reference electrode.

[0068] Image 6 In order to use the all-solid urease electrode prepared in this implementation to measure the potential response standard curve obtained by different concentrations of urea solution, the standard equation is ΔE(mV)=24.471c(mmol/L)-15.514. As shown by the straight line in the figure, ΔE represents the response electromotive force, c represents the concentration of urea in the solution, and the correlation coefficient R2 is 0.9911.

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Owner:ZHONGYUAN ENGINEERING COLLEGE

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quick response
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Full-solid urase electrode and preparation method thereof

An all-solid-state, urease technology, applied in the field of electroanalytical chemical detection, can solve the problems of long time, high cost, need to add reagents, etc., and achieve the effects of fast response, low production cost, and simple production.

AI-Extracted Technical Summary

Problems solved by technology

Abstract

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Examples

Example Embodiment

Example Embodiment

Example Embodiment

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Description & Claims & Application Information

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