Full-solid urase electrode and preparation method thereof
An all-solid-state, urease technology, applied in the field of electroanalytical chemical detection, can solve the problems of long time, high cost, need to add reagents, etc., and achieve the effects of fast response, low production cost, and simple production.
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[0039] Example 1
[0040] The preparation process and steps in this embodiment are as follows:
[0041] (1) A layer of silver paste is printed on the polyvinyl chloride vinyl board by a screen printing method. The silver paste layer is divided into the first contact electrode 2-1, the second contact electrode 2-2, the conductive layer 9, and the first reaction zone 10. -1 and the second reaction zone 10-2;
[0042] (2) Preparation of reference electrode: prepare bovine serum albumin into an aqueous solution with a mass concentration of 5%; add nano-MnO 2 Disperse in the carbon slurry to form a mixture, nano-MnO 2 The mass concentration in the carbon paste is 30%; a 12% bovine serum albumin solution by weight of the mixture is added to the mixture to prepare the second response layer slurry, and the response layer slurry is printed by screen printing. In the second reaction zone 10-2, the second response layer 4-2 is printed to completely cover the second reaction zone 10-2 and becom...
Example Embodiment
[0049] Example 2
[0050] The preparation process and steps in this embodiment are as follows:
[0051] (1) A layer of silver paste is printed on the polyvinyl chloride vinyl board by a screen printing method. The silver paste layer is divided into the first contact electrode 2-1, the second contact electrode 2-2, the conductive layer 9, and the first reaction zone 10. -1 and the second reaction zone 10-2;
[0052] (2) Preparation of reference electrode: prepare bovine serum albumin into an aqueous solution with a mass concentration of 5%; add nano-SnO 2 Disperse in carbon paste to form a mixture, nano-SnO 2 The mass concentration in the carbon paste is 20%; a bovine serum albumin solution of 7% by weight of the mixture is added to the mixture to prepare the second response layer slurry, and the response layer slurry is printed by screen printing. In the second reaction zone 10-2, the second response layer 4-2 is printed to completely cover the second reaction zone 10-2 and become t...
Example Embodiment
[0059] Example 3
[0060] The preparation process and steps in this embodiment are as follows:
[0061] (1) A layer of silver paste is printed on the polyvinyl chloride vinyl board by a screen printing method. The silver paste layer is divided into the first contact electrode 2-1, the second contact electrode 2-2, the conductive layer 9, and the first reaction zone 10. -1 and the second reaction zone 10-2;
[0062] (2) Preparation of reference electrode: prepare bovine serum albumin into an aqueous solution with a mass concentration of 5%; add nano-RuO 2 Disperse in the carbon paste to form a mixture, nano-RuO 2 The mass concentration in the carbon paste is 10%; a 2% bovine serum albumin solution by weight of the mixture is added to the mixture to prepare the second response layer slurry, and the response layer slurry is printed by screen printing. In the second reaction zone 10-2, the second response layer 4-2 is printed to completely cover the second reaction zone 10-2 and become ...
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