Cellulose and xylan bifunctional enzyme, coding gene thereof and application

A bifunctional enzyme and xylan technology, applied in the field of genetic engineering, can solve the problem of less research on bifunctional enzymes, and achieve the effects of improving feed utilization, reducing chyme viscosity, and increasing reproduction

Active Publication Date: 2013-06-12
ANHUI AGRICULTURAL UNIVERSITY
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, there are still few studies on bifunctional enzymes in the rumen (except Chang Lei et al. (Lei Chang, Mozhu Ding, Lei Bao, Yingzhi Chen, Jungang Zhou, Hong Lu. Characterization of a bifunctional xylanase / endoglucanase from yak rumen microorganisms. Microbiol Biotechnol (2011) 90: 1933-1942))

Method used

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  • Cellulose and xylan bifunctional enzyme, coding gene thereof and application
  • Cellulose and xylan bifunctional enzyme, coding gene thereof and application
  • Cellulose and xylan bifunctional enzyme, coding gene thereof and application

Examples

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Embodiment Construction

[0034] Materials used in the following examples include: Escherichia coli strain EPI300 and plasmid pIndigoBAC-5 (purchased from Epicentre); Escherichia coli strains DH5α and BL21 (DE3) (purchased from TransGen Company) ); cloning vector pUC19 / Bam HI and restriction endonuclease (purchased from Fermentas); expression vector pET28a(+) (purchased from Novengen); polymerase (purchased from Takara).

[0035] First, the acquisition of cellulase-encoding genes

[0036] 1) Construction of goat rumen microbial metagenomic library

[0037] Anhui white goat rumen fluid was collected, microbial cells were isolated, and genomic DNA was prepared by embedding and lysing in low-melting agarose. The obtained DNA was partially digested with Hind III, and the DNA fragments of more than 50 kb were recovered and connected with BAC vector to construct goat rumen Microbial metagenomic library (Anna, Li Lumu, Xu Fazhi, Cheng Jianbo. Construction and analysis of goat rumen microbial metagenomic BAC ...

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Abstract

The invention discloses a cellulose and xylan bifunctional enzyme, a coding gene thereof and application. Cel28a has a nucleotide sequence or a homologous sequence showed in SEQID NO.1, and the homologous sequence has 51% of homology with an amino acid sequence showed in SEQIDNO.2. The total length of the gene is 1596bp, and the cellulose and xylan bifunctional enzyme containing 531 amino acids and signal peptide containing 22 amino acids are coded. The cellulose and xylan bifunctional enzyme has certain heat resistance, and can be used as a feed additive for industries such as feed and the like.

Description

[technical field] [0001] The invention relates to the field of genetic engineering, in particular to a bifunctional enzyme of cellulose and xylan, its encoding gene and application. [Background technique] [0002] Cellulose, hemicellulose and lignin are the main components of plant cell walls, and cellulose accounts for about 40-45% of cell dry weight. Cellulose is the most widely distributed and abundant carbon source on the earth, accounting for more than 50% of the carbon content in the plant kingdom. Cellulase is a general term for a group of enzymes that can hydrolyze cellulose into glucose, and is divided into endo-β-1,4-glucanase (endo-β-1,4-glucanase, EC3.2.1 .4), exoglucanase (exoglucanase, also known as cellobiohydrolase, EC3.2.1.91) and β-glucosidase (β-glucosidase, EC3.2.1.21) (TOMME P, WARRENRAJ , GIL KESN R. Cellulose hydrolysis by bacteria and fungi. Adv Microbiol Physiol, 1995, 37: 1-811). Lignin accounts for approximately 15-25% of dry cell weight and is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/56C12N9/42C12N15/63C12N1/21A23K1/165C12R1/19
Inventor 程建波范彩云姜海琴王力生章孝荣李吕木张运海张子军蔡海莹
Owner ANHUI AGRICULTURAL UNIVERSITY
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