Unlock instant, AI-driven research and patent intelligence for your innovation.

Primer-probe combinations and kits for the specific detection and identification of Neisseria meningitidis

A technology of Neisseria inflammatory and Neisseria, which is applied in the field of oligonucleotide sequence combination, can solve the problems of increased time and cost of detection, and the risk of pathogenicity cannot be completely excluded, so as to save the cost of detection. Effect

Active Publication Date: 2016-08-10
JIANGSU UNINOVO BIOLOGICAL TECH
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the actual detection process, for a sample to be tested, if only detecting whether there is group A Neisseria meningitidis, or on the contrary, only detecting whether there is group C Neisseria meningitidis, the pathogenicity cannot be completely ruled out. Danger
However, if two tests are performed separately, obviously, the time cost of the test will be greatly increased.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Primer-probe combinations and kits for the specific detection and identification of Neisseria meningitidis
  • Primer-probe combinations and kits for the specific detection and identification of Neisseria meningitidis
  • Primer-probe combinations and kits for the specific detection and identification of Neisseria meningitidis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] Preferred implementations of the present invention will be described in detail below in conjunction with specific examples. It should be pointed out that the examples listed here are for the purpose of illustration only, and should not be construed as any limitation on the scope of the present invention. The reagent kits, buffers and other reagents used therein are only reagents specifically selected in this specific example. It should be understood that those skilled in the art can select corresponding reagents from other companies as needed to achieve the purpose of the present invention.

[0030] 1. Design and synthesis of primers and TaqMan probes

[0031] Use the Blast tool to analyze the genome sequences of Neisseria meningitidis (common), Neisseria meningitidis group A and Neisseria meningitidis group C in Genbank and domestic and foreign literature, and select their stable conserved regions as the detection target sequence. Various serogroups of Neisseria mening...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an oligonucleotide sequence combination for specifically detecting the nucleic acid of Neisseria meningitidis in a sample by using a real-time fluorescent PCR method, and a kit comprising the combination. Utilizing the sequence combination or kit of the present invention, it is possible to quickly detect whether there is Neisseria meningitidis nucleic acid in a sample, and at the same time identify whether it is the most popular group A or group C in my country. The detection limit of the kit for Neisseria meningitidis (common) is 200 copies per reaction system. The limit of detection for Neisseria meningitidis group A is 200 copies per reaction, and for Neisseria meningitidis group C is 200 copies per reaction. The whole reaction can be completed within 2 hours, and has important application value in the fields of disease monitoring and clinical diagnosis.

Description

technical field [0001] The invention relates to an oligonucleotide sequence combination for specifically detecting the nucleic acid of Neisseria meningitidis in a sample by using a real-time fluorescent PCR method, and a kit comprising the combination. Utilizing the sequence combination or kit of the present invention, it is possible to quickly detect whether there is Neisseria meningitidis nucleic acid in a sample, and at the same time identify whether it is the most popular group A or group C in my country. The detection limit of the kit for Neisseria meningitidis (common) is 200 copies per reaction system, the detection limit for group A Neisseria meningitidis is 200 copies per reaction system, and for group C Neisseria meningitidis The detection limit of bacteria was 200 copies per reaction system. The whole reaction can be completed within 2 hours, and has important application value in the fields of disease monitoring and clinical diagnosis. technical background [0...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11C12R1/36
Inventor 文锋其他发明人请求不公开姓名
Owner JIANGSU UNINOVO BIOLOGICAL TECH
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com