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Preparation method of achyranthes bidentata saponin

A technique for hyssopus saponins and hyssopus saponins, which is applied in the field of preparation of hyssopus saponins, can solve the problems of few extraction and purification methods of hyssopus saponins, toxicity of organic solvents and high cost, and achieves short extraction time, high extraction efficiency and easy amplification. Effect

Inactive Publication Date: 2012-08-29
苏州宝泽堂医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are few extraction and purification methods for Achyranthes saponins, and traditionally, alcohol extraction and n-butanol extraction are used, which has the disadvantages of high cost and toxic residual organic solvents

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Grind the Achyranthes bidentata medicinal material, take 2kg, add appropriate amount of distilled water and 6g of biological enzymes for natural enzymolysis to obtain enzymolysis raw materials, put the enzymolysis raw materials in microwave extraction equipment, add 5 times distilled water, microwave extraction power 600W, extract 2 times, Centrifuge to obtain the extract, absorb it through D-4020 macroporous adsorption resin column, elute impurities with 8L distilled water, then elute with 5%, 15%, 40%, 50% ethanol solution, and collect 40-55% ethanol solution for elution liquid, passed through the D-280 column, collected the lower column liquid, concentrated into an extract, refluxed with absolute ethanol to dissolve, filtered while hot, placed crystals, filtered out the crystals, recrystallized twice with absolute ethanol, and dried in vacuum to obtain high-purity cattle Knee saponin 16.8g, content is 90.3%.

Embodiment 2

[0019] Grind the Achyranthes bidentata medicinal material, take 2kg, add appropriate amount of distilled water and 12g of biological enzymes for natural enzymolysis to obtain enzymolysis raw materials, put the enzymolysis raw materials in microwave extraction equipment, add 7 times distilled water, microwave extraction power 400W, extract 2 times, Centrifuge to obtain extract, absorb through NKA-9 macroporous adsorption resin column, elute impurities with 10L distilled water, then elute with 8%, 15%, 40%, 55% ethanol solution, collect 4055% ethanol solution eluate, Pass through the D-280 column, collect the lower column liquid, concentrate into extract, reflux with absolute ethanol to dissolve, filter while hot, place the crystals, filter out the crystals, recrystallize with absolute ethanol for 3 times, and vacuum dry to obtain high-purity achyranthes saponins 18.7g, the content is 89.7%.

Embodiment 3

[0021] Grind the Achyranthes bidentata medicinal material, take 2kg, add appropriate amount of distilled water and 15g of biological enzymes for natural enzymolysis to obtain enzymolysis raw materials, put the enzymolysis raw materials in microwave extraction equipment, add 5 times distilled water, microwave extraction power 500W, extract twice, Centrifuge to obtain the extract, absorb through X-5 macroporous adsorption resin column, elute impurities with 8L distilled water, then elute with 15%, 25%, 45%, 55% ethanol solution, collect 40-55% ethanol solution for elution liquid, passed through the D-280 column, collected the lower column liquid, concentrated into an extract, refluxed with absolute ethanol to dissolve, filtered while hot, placed crystals, filtered out the crystals, recrystallized twice with absolute ethanol, and dried in vacuum to obtain high-purity cattle Knee saponin 15.9g, content is 92.6%.

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Abstract

The invention relates to a preparation method of achyranthes bidentata saponin. The preparation method comprises the following steps: crushing dry achyranthes bidentata; adding a proper amount of distilled water and 0.3-0.8% of bioenzyme, and performing natural enzymolysis to obtain an enzymolyzed raw material; performing microwave extraction, and centrifugating to obtain an extraction solution; adsorbing the extraction solution by using a macroporous resin column; decolorizing the extraction solution by using D-280 resin; concentrating liquid in a lower column into extract; refluxing and dissolving the extract by using absolute ethanol; standing and crystallizing; recrystallizing; filtering out crystals; and drying the crystals to obtain the achyranthes bidentata saponin. The preparation method has the advantages of simple operation, high yield and high purity.

Description

Technical field: [0001] The invention relates to a method for extracting and preparing saponins from plants, in particular to a method for preparing achyranthes saponins. Background technique: [0002] Radix Achyranthis Bidentatae is the root of Radix Achyranthis Bidentatae. Distributed in Africa, Russia, Vietnam, India, Malaysia, the Philippines, North Korea, and all parts of China except Northeast China. It has the effects of invigorating the liver and kidney, strengthening the bones, promoting blood circulation, activating the fire, diuresis and treating stranguria. It is used for soreness of the waist and knees, flaccidity of the lower limbs, amenorrhea due to blood stagnation, dysmenorrhea, postpartum blood stasis, abdominal pain, abdominal pain, lumps, retained clothes, fever Drenching, bloody stranguria, bruises, carbuncle and malignant sores, sore throat and other diseases. The roots contain saponins, mainly triterpenoid saponins. Wang Lijing's research on Achyran...

Claims

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Application Information

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IPC IPC(8): A61K36/21A61P9/00A61P15/04A61P29/00A61P35/00
Inventor 李法庆刘东锋
Owner 苏州宝泽堂医药科技有限公司