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Method for quickly propagating angelica keiskei koidzmi

A fast, petiole technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of undifferentiated somatic embryos, difficult rooting of regenerated plants, long culture period, etc. The effect of short cultivation period and easy rooting

Inactive Publication Date: 2012-09-12
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned methods all obtain regenerated plants by clustering buds, which have the disadvantages of difficult rooting of regenerated plants, low seedling rate, and long cultivation period.
After searching, there is no report on the differentiation of somatic embryos from callus and regeneration of plants

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The specific plan is: take the leaf with handle of Ashitaba, divide it into two parts, the leaf and the petiole, the petiole has no bud point, wash it in running water for 3-5 times, and then wash it twice in tap water with washing powder, wash it with Sterilize with 75% alcohol for 10 seconds. Leaves and petioles are sterilized by 1% sodium hypochlorite for 3 minutes and 5 minutes respectively, washed 3-5 times with sterile water, and then placed on sterile filter paper to blot dry. Cut the leaves into 0.5cm*0.5cm size, cut the petiole into 0.5cm size, transfer to the MS basic medium with 0.5mg / L zeatin and 0.5mg / L NAA, pH value 5.6, that is, add The production of callus is induced on the MS medium with hormones, the culture temperature is 25°C, the light time is 12h, and somatic embryos are differentiated from the callus in 20-25 days, and new plants are regenerated by germination. The regenerated new plants above 0.2 cm are transferred to the rooting medium to take r...

Embodiment 2

[0025] The specific plan is: take the leaf with handle of Ashitaba, divide it into two parts, the leaf and the petiole, the petiole has no bud point, wash it in running water for 3-5 times, and then wash it twice in tap water with washing powder, wash it with Sterilize with 75% alcohol for 10 seconds. Leaves and petioles are sterilized by 1% sodium hypochlorite for 3 minutes and 5 minutes respectively, washed 3-5 times with sterile water, and then placed on sterile filter paper to blot dry. Cut the leaves into 0.5cm*0.5cm size, cut the petiole into 0.5cm size, transfer to MS basic medium with 3mg / L zeatin and 0.7mg / L NAA, pH value 5.8, that is, add hormone The callus is induced on the MS medium, the culture temperature is 25°C, the light time is 12h, and the somatic embryos are differentiated from the callus in 20-25 days, and new plants are regenerated by germination. The regenerated new plants above 0.2 cm are transferred to the rooting medium to take root. The rooting medi...

Embodiment 3

[0027] The specific plan is: take the leaf with handle of Ashitaba, divide it into two parts, the leaf and the petiole, the petiole has no bud point, wash it in running water for 3-5 times, and then wash it twice in tap water with washing powder, wash it with Sterilize with 75% alcohol for 10 seconds. Leaves and petioles are sterilized by 1% sodium hypochlorite for 3 minutes and 5 minutes respectively, washed 3-5 times with sterile water, and then placed on sterile filter paper to blot dry. After the leaves were cut into 0.5cm*0.5cm size and the petiole was cut into 0.5cm size, they were transferred to MS basic medium with 5 mg / L zeatin and 1 mg / L NAA and a pH value of 6.0, that is, hormone-added The generation of callus is induced on MS medium, the culture temperature is 25°C, the light time is 12h, and somatic embryos are differentiated from the callus in 20-25 days, and new plants grow out of regeneration after germination. The regenerated new plants above 0.2 cm are transf...

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Abstract

The invention discloses a method for quickly propagating angelica keiskei koidzmi. The method comprises the following steps that on a hormone-containing Murashige and Skoog (MS) culture medium, leaves and stems of the angelica keiskei koidzmi are induced to grow callus tissues and form somatic embryos further; the somatic embryos burgeon to form new plants; and the new plants grow roots on the rooting culture medium. A liter of hormone-containing MS culture medium comprises the components of 0.5 to 5mg of zeatin, 0.5 to 1mg of NAA and MS minimal medium. The method has the advantages of overcoming the defects of the conventional methods for propagating angelica keiskei koidzmi by tissue culture and realizing the rapid propagation of the angelica keiskei koidzmi; and moreover, the culture period is short, regenerated plants easy grow root, and seedling emergence rate is high. The method can be applied to the high-efficiency propagation of the angelica keiskei koidzmi.

Description

technical field [0001] The invention relates to a method for propagating plants, in particular to a method for rapidly propagating Ashitaba, and belongs to the field of biotechnology. Background technique [0002] Ashitaba (Angelica keiskei koidzmi), also known as Hachijo grass, Haifeng ginseng, and longevity vegetable, is full of vitality. It is picked today and sprouts tomorrow. It is named because of this strong vitality and the origin of Hachijo Island in Japan. The scientific name of Ashitaba is Angdica Uterus, the genus name is the origin of the Latin angel, and the species name means useful, implying outstanding medicinal effects. Ashitaba is a perennial herbaceous plant belonging to the cress family. It likes a warm and humid climate. The production area is centered on the Izu Seven Islands and extends to the front of the Izu Peninsula, the Miura Peninsula, and the Kii Peninsula. If you have the opportunity to visit the Izu Seven Islands or the Izu Peninsula, you ca...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 曹越平薄路花金加清
Owner SHANGHAI JIAO TONG UNIV
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