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Method for extracting and separating agapanthussaponin A

A separation method and saponin technology, which is applied in the field of extraction and separation of saponin Ⅲ, can solve problems such as saponin Ⅲ that have not been seen yet, and achieve the effect of reducing sample loss, large amount of preparation, and good separation effect

Inactive Publication Date: 2012-09-12
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are no relevant literatures and patent reports on the industrial extraction and purification method of saponin Ⅲ in China

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The underground part of the broad-leaved soil Ophiopogon japonicus is crushed, weighed 3kg, and added to a supercritical extraction kettle for supercritical CO extraction. 2 Extraction, extraction temperature is 60°C, extraction pressure is 25MPa, analysis temperature is 50°C, analysis pressure is 9MPa, CO 2 The flow rate is 20L / h, remove oily substances, release raw material residues, add 80% ethanol solution for microwave extraction 3 times, microwave extraction power is 800w, filter to obtain extract, concentrate to extract, add appropriate amount of water to disperse, add 3 Extract twice the amount of water-saturated n-butanol twice, add 0.5 times the amount of acetone to the combined extracts, filter, concentrate and dry the filtrate to obtain the crude product; use petroleum ether-ethyl acetate-ethanol-water (8:4:7:6) As a two-phase solvent system, after mixing, put it in a separatory funnel and let it stand for stratification. Take the upper phase as the stationa...

Embodiment 2

[0020] The underground part of the broad-leaved soil Ophiopogon japonicus is crushed, weighed 3kg, and added to a supercritical extraction kettle for supercritical CO extraction. 2 Extraction, extraction temperature is 40°C, extraction pressure is 15MPa, analysis temperature is 30°C, analysis pressure is 5MPa, CO 2 The flow rate is 18L / h, remove oily substances, release raw material residues, add 70% ethanol solution for microwave extraction 3 times, microwave extraction power is 600w, filter to obtain extract, concentrate to extract, add appropriate amount of water to disperse, add 1 Extract three times the amount of water-saturated n-butanol, combine the extracts and add 1 times the amount of acetone to precipitate, filter, concentrate and dry the filtrate to obtain the crude product; use petroleum ether-ethyl acetate-ethanol-water (6:4:7:6) As a two-phase solvent system, after mixing, put it in a separatory funnel and let it stand for stratification. Take the upper phase as...

Embodiment 3

[0022] The underground part of the broad-leaved soil Ophiopogon japonicus is crushed, weighed 3kg, and added to a supercritical extraction kettle for supercritical CO extraction. 2 extraction, extraction temperature is 50°C, extraction pressure is 20MPa, analysis temperature is 40°C, analysis pressure is 7MPa, CO 2 The flow rate is 20L / h, remove oily substances, release raw material residues, add 70% ethanol solution for microwave extraction twice, microwave extraction power is 800w, filter to obtain extract, concentrate to extract, add appropriate amount of water to disperse, add 2 Double the amount of water-saturated n-butanol and extract twice, combine the extracts and add 0.8 times the amount of acetone to precipitate, filter, concentrate and dry the filtrate to obtain the crude product; use petroleum ether-ethyl acetate-ethanol-water (7:3:6:5) As a two-phase solvent system, after mixing, put it in a separatory funnel and let it stand for stratification. Take the upper pha...

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PUM

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Abstract

The invention relates to a method for extracting and separating agapanthussaponin A, which comprises the following steps of: crushing the underground part of broadleaf liriope, and adding to a supercritical extraction kettle for supercritical CO2 extraction, removing grease substances, and discharging raw material residues; adding a 50-80% ethanol solution for microwave extraction; concentrating an extracting solution to obtain an extract; after dispersion by adding right amount of water, adding water-saturated n-butanol for extracting for 2-3 times; combining extracts and adding acetone precipitation, filtering, and concentrating and drying a filtrate to obtain a crude product; and separating and extracting the crude product by using high-speed countercurrent chromatography to obtain the agapanthussaponin A. The method has the advantages of large amount of preparation, low sample loss, short production cycle, high efficiency and less pollution, and is suitable for industrial production.

Description

technical field [0001] The invention belongs to the field of separation of natural products, and in particular relates to a method for extracting and separating dinoside III. Background technique [0002] Agapanthus saponin Ⅲ (Agapanthussaponin A) is a steroidal saponin with molecular formula C 45 h 72 o 16 , Molecular weight 869.06, soluble in n-butanol, methanol, ethanol, insoluble in acetone. Ficus saponin III has anti-inflammatory and hypoglycemic activity. The main source of saponin Ⅲ is Liliaceae (Liliaceae) broad-leaved soil Ophiopogon japonicus Liriope platyphylla The underground part of Wang et Tang. [0003] At present, there are no relevant literatures and patent reports on the industrial extraction and purification method of forgoside Ⅲ in China. Contents of the invention [0004] The technical problem to be solved by the present invention is to provide a method for extracting and separating the saponin III, which is simple in operation and high in produ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J71/00
CPCY02P20/54
Inventor 刘东锋万冬梅
Owner NANJING ZELANG MEDICAL TECH
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