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Fermentation of carbohydrate

A carbohydrate, composition technology, applied in the direction of fermentation, microorganism-based methods, methods using microorganisms, etc., can solve problems such as concentration reduction

Active Publication Date: 2012-09-12
ANITOX CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0035] Only laboratory studies have shown that antibodies, sulfite and peroxide products are beneficial for the control of Lactobacillus, but the problem with these products is that the concentration decreases due to oxidation and breakdown of these chemicals, which will require constant monitoring throughout the fermentation process to ensure the effective concentration

Method used

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  • Fermentation of carbohydrate
  • Fermentation of carbohydrate
  • Fermentation of carbohydrate

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0108] This example represents the formulation based on the formaldehyde product used in the subsequent examples

[0109]

example 2

[0111] The purpose of this study was to determine the effect of formulation A on the survival of Lactobacillus.

[0112] Materials and methods:

[0113] Lactobacillus plantarum (B-4496) was obtained from USDA-Microbial Genomics and Bioprocessing Research, Illinois. Lactobacillus plantarum at Difco TM Lactobacillus MRS (Mati-Rogosa-Sharpe) liquid medium was grown. The liquid medium was diluted with sterilized peptone water to obtain different concentrations of Lactobacillus. Each dilution was treated with different concentrations of formulation A (0, 1, 2 and 3 kg / MT) and incubated at room temperature (20°C) for 24 hours. After incubation, samples were taken in triplicate, and plated in a medium containing 1.5% Difco TM Agar Granulated solidifying agent (Agar Granulated solidifying agent) on the MRS liquid medium. Plates were incubated overnight at 37°C and colonies counted after 24 hours. The mean cfu / mL for each treatment is shown in the table below:

[0114]

[011...

example 3

[0118] The purpose of this study was to determine the effect of formulation A on the survival of yeast and Lactobacillus during fermentation.

[0119] Materials and methods:

[0120] Sterilized ground corn was mixed with sterilized water in a glass fermenter. Next, a commercially available enzyme solution (Stargen: Genencor) containing a blend of alpha-amylase and glucoamylase for the treatment of starch for cooking was added. Active dry yeast (Fali Yeast) (10 10 cfu / g; Fleischmann). Finally, using the Difco Microbial Genomics and Bioprocesses TM Lactobacillus plantarum (B-4496) grown in Lactobacillus MRS broth as a representative bacterial contaminant of the fermentor. Formaldehyde-based products are added as the final step in the process.

[0121] The treatments used are shown in the table below. Samples taken at 4 hours, 24 hours, 48 ​​hours, 72 hours and 96 hours were analyzed for yeast and lactobacillus numbers. Each process is as follows:

[0122]

[0123] The...

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PUM

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Abstract

A high yield method for fermenting carbohydrate to ethanol, comprising a) treating carbohydrate with a composition containing 10 - 90 wt % of an aldehyde selected from the group consisting of formaldehyde, para-formaldehyde, glutaraldhyde and mixtures thereof, 1 - 50 wt % of a surfactant having an I JLB from 4 to 18, 0 - 20 wt % of an antimicrobial terpene, or essential oils, 1- 50 wt % of organic acids selected from C1-24 fatty acids, their salts, and glyceride esters thereof, and 1- 50 wt % water, b ) fermenting said carbohydrate in the presence of yeast in a fermentation broth, and c) isolating ethanol in a higher yield than would be obtained without step a).

Description

technical field [0001] The present invention relates to a high yield process for the production of ethanol by fermentation of carbohydrates by treating incoming carbohydrates with aldehydes, fatty acids, terpenes and surfactants. Background technique [0002] In 2009, the Renewable Fuels Standard (RFS) called for putting 11.1 billion gallons of ethanol and other biofuels into the US auto fuel market to meet future demand. This will lead to an increase in the industrial demand for corn and also require an increase in planting capacity. In the past year, U.S. production capacity has increased by 2.7 billion gallons a year, a 34 percent increase over 2007. This increase in production capacity is achieved through the completion, start-up and operation of new ethanol distillation plants. [0003] Ethanol is a promising biofuel from renewable sources, produced from starches of grains (maize, sorghum, wheat, triticale, rye, germinated barley, rice), tuber crops (potatoes) or dire...

Claims

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Application Information

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IPC IPC(8): C12P7/10A23K1/12C12N1/20C12N1/22C12R1/225A23K10/32A23K10/38
CPCC12P7/10Y02E50/10
Inventor 胡里奥·皮门特尔詹姆士·D·威尔逊
Owner ANITOX CORP