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Heat-resisting freeze-dried protective agent for live vaccine for chicken infectious bursal diseases and preparation method for heat-resisting freeze-dried protective agent

A technology of bursal disease and chicken infectivity, applied in antiviral agents, pharmaceutical formulas, virus antigen components, etc., can solve the problems of reducing production costs, long-term storage and transportation of live bursal disease vaccines, and achieves virus The effect of low loss rate and high degree of aging resistance

Inactive Publication Date: 2012-09-19
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The invention provides a heat-resistant freeze-drying protective agent for chicken infectious bursal disease live vaccine. Technical problems of long-term storage and transportation at 2°C to 8°C, and simplify the preparation method to reduce production costs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Preparation of Heat-resistant Freeze-dried Vaccine of Chicken Infectious Bursal Disease Live Vaccine

[0024] 1) Mix carboxymethyl cellulose sodium with water for injection, heat at 98-100°C for 10-20 minutes, cool to room temperature, and prepare a concentration of 6% (the concentration involved in the present invention is all unless otherwise specified. is the mass percent concentration) of liquid A.

[0025] 2) Mix xylooligosaccharides, sorbitol and water for injection to prepare liquid B with a concentration of 9% xylooligosaccharides.

[0026] 3) Mix polyvinylpyrrolidone, sodium glutamate, sodium selenite, and potassium chloride with water for injection, stir and mix well, and prepare liquid C with 6% polyvinylpyrrolidone and 2.4% sodium glutamate , Sodium selenite concentration 1.2%, potassium chloride 0.03%.

[0027] 4) Prepare liquid A, liquid B, and liquid C at a volume ratio of 1:1:1 to make a vaccine freeze-drying protective agent.

[0028] 5) Mix the vacc...

Embodiment ２

[0032] Preparation of Heat-resistant Freeze-dried Vaccine of Chicken Infectious Bursal Disease Live Vaccine

[0033] 1) Mix carboxymethylcellulose sodium with water for injection, heat at 98-100°C for 10-20 minutes, cool to room temperature, and prepare liquid A with a concentration of 9%.

[0034] 2) Mix xylooligosaccharides, sorbitol and water for injection to prepare liquid B, with a concentration of xylooligosaccharides of 12%.

[0035] 3) Mix polyvinylpyrrolidone, sodium glutamate, sodium selenite, and potassium chloride with water for injection, stir and mix well, and prepare liquid C with 6% polyvinylpyrrolidone and 2.4% sodium glutamate , Sodium selenite concentration 1.5%, potassium chloride 0.1%.

[0036] 4) Prepare liquid A, liquid B, and liquid C at a volume ratio of 1:1:1 to make a vaccine freeze-drying protective agent.

[0037] 5) Mix the vaccine lyoprotectant with the virus liquid of chicken infectious bursal disease live vaccine at a volume ratio of 4:1.

...

Embodiment ３

[0041] Preparation of Heat-resistant Freeze-dried Vaccine of Chicken Infectious Bursal Disease Live Vaccine

[0042] 1) Mix carboxymethylcellulose sodium with water for injection, heat at 98-100°C for 10-20 minutes, cool to room temperature, and prepare liquid A with a concentration of 9%.

[0043] 2) Mix xylooligosaccharides, sorbitol and water for injection to prepare liquid B, with a concentration of xylooligosaccharides of 18%.

[0044] 3) Mix polyvinylpyrrolidone, sodium glutamate, sodium selenite, potassium chloride with water for injection, stir and mix, and prepare liquid C, polyvinylpyrrolidone 6%, sodium glutamate concentration 1.8%, sub Sodium selenate concentration 1.2%, potassium chloride 0.15%.

[0045] 4) Prepare liquid A, liquid B, and liquid C at a volume ratio of 1:1:1 to make a vaccine freeze-drying protective agent.

[0046] 5) Mix the vaccine lyoprotectant with the virus liquid of chicken infectious bursal disease live vaccine at a volume ratio of 5:1. ...

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Abstract

The invention relates to a heat-resisting freeze-dried protective agent for a live vaccine for chicken infectious bursal diseases and a preparation method for the heat-resisting freeze-dried protective agent. The heat-resisting freeze-dried protective agent for the live vaccine for chicken infectious bursal diseases is prepared from the following components in percentage by weight: 2 to 3 percentof sodium carboxy methyl cellulose, 3 to 6 percent of xylo-oligosaccharide, 2 to 3 percent of polyvinylpyrrolidone, 0.5 to 0.8 percent of glutamine, 0.1 to 0.5 percent of sodium selenite, 0.01 to 0.05 percent of potassium chloride and the balance of water for injection. By the heat-resisting freeze-dried protective agent, the technical problem that the live vaccine for chicken infectious bursal diseases cannot be stored and transported for long time at the temperature of between 2 and 8 DEG C is solved, production process is simplified, and production cost is reduced.

Description

[0001] technical field [0002] The invention relates to a heat-resistant freeze-drying protective agent for chicken infectious bursal disease live vaccine and a preparation method thereof, belonging to the field of animal vaccine production. [0003] Background technique [0004] Freeze-drying technology is an important application technology in biology. It is often used in the scientific research and production of biological products to maintain the biological characteristics of bacteria and viruses, stabilize the biological activity of biological raw materials and semi-finished products, and extend the validity period of finished products. Lyoprotectant is a key technology in the production of live vaccines, which has a great impact on the storage, transportation and use of vaccines. [0005] The freeze-dried products of foreign live vaccines have a shelf life of more than 15 months at 2-8°C, but the formula of the protective agent is strictly confidential. Among animal ...

Claims

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Application Information

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IPC IPC(8): A61K47/38A61K47/26A61K47/04A61K39/12A61P31/14
Inventor 郝智慧曲宝涵苑国春丁兆鹏杨芬芳
Owner QINGDAO AGRI UNIV
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