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Method for preparing antioxidant peptide from shrimp shell

A technology of antioxidant peptides and shrimp shells, applied in the biological field, can solve problems such as unreported research on antioxidant peptides, and achieve the effects of enhancing human immunity, non-toxic side effects, and strong free radical scavenging ability.

Inactive Publication Date: 2012-09-19
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Domestic research on the preparation of antioxidant peptides by hydrolyzing shrimp shells with protease has not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A) Wash the shrimp shells to remove impurities, dry them at 40°C for 20 hours, grind them with a pulverizer, pass through a 40-mesh sieve to make shrimp shell powder, and then add water to prepare a shrimp shell powder solution with a mass percentage of 6.5%;

[0022] B) Adjust the pH of the shrimp shell powder solution to 11.0, then add protease 3500U / g, hydrolyze in a water bath at 55°C for 1 hour, carry out the hydrolysis under stirring, and keep the temperature and pH constant to obtain the shrimp shell protein hydrolyzate;

[0023] C) Shrimp shell protein hydrolyzate was inactivated in a water bath at 85°C for 10 minutes. After cooling to room temperature, it was centrifuged at 3000r / min for 10 minutes. The supernatant was taken and ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 5000Da. Obtain the ultrafiltrate of shrimp shell polypeptide;

[0024] D) The shrimp shell peptide ultrafiltrate is subjected to vacuum evaporation conce...

Embodiment 2

[0027] A) Wash the shrimp shells to remove impurities, dry them at 60°C for 26 hours, grind them with a pulverizer, pass through a 100-mesh sieve to make shrimp shell powder, and then add water to make a 6% shrimp shell powder solution;

[0028] B) Adjust the pH of the shrimp shell powder solution to 10.5, then add protease 3500U / g, hydrolyze in a water bath at 50°C for 4 hours, carry out the hydrolysis under stirring, and keep the temperature and pH constant to obtain the shrimp shell protein hydrolyzate;

[0029] C) Shrimp shell protein hydrolyzate was inactivated in a water bath at 100°C for 20 minutes. After cooling to room temperature, it was centrifuged at 6000r / min for 20 minutes. The supernatant was taken and ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 5000Da. Obtain the ultrafiltrate of shrimp shell polypeptide;

[0030] D) The shrimp shell peptide ultrafiltrate is subjected to vacuum evaporation concentration and vacuum freeze-d...

Embodiment 3

[0033] A) Wash the shrimp shells to remove impurities, dry them at 50°C for 24 hours, grind them with a pulverizer, pass through a 60-mesh sieve to make shrimp shell powder, and then add water to prepare a shrimp shell powder solution with a mass percentage of 6.5%;

[0034] B) Adjust the pH of the shrimp shell powder solution to 10, then add alkaline protease 4500U / g, and hydrolyze it in a water bath at 50°C for 2.5 hours. The hydrolysis is carried out under stirring, and the temperature and pH are kept constant to obtain the hydrolyzed shrimp shell protein. liquid;

[0035] C) Shrimp shell protein hydrolyzate was inactivated in a water bath at 95°C for 15 minutes, cooled to room temperature, and centrifuged at 4000r / min for 15 minutes, and the supernatant was taken and filtered with an ultrafiltration membrane with a molecular weight cut-off of 5000Da Ultrafiltration to obtain the shrimp shell polypeptide ultrafiltrate;

[0036] D) The shrimp shell peptide ultrafiltrate is...

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PUM

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Abstract

The invention discloses a method for preparing antioxidant peptide from shrimp shell. The method comprises the steps that: shrimp shell obtained as a byproduct from shrimp processing is used as a raw material, then dried, ground and sieved, and added with water to prepare a shrimp shell powder solution with a certain mass fraction, the pH of the solution is regulated and then alkaline proteinase is added for hydrolysis at constant temperature, enzyme inactivation, cooling and centrifugation are carried out after the reaction ends, and supernatant is subjected to ultrafiltration and then concentrated and freeze-dried to obtain shrimp shell-type antioxidant peptide. Compared with the existing antioxidants, the antioxidant peptide disclosed by the invention is simple in preparation method, rich and highly-available in raw material which is shrimp shell obtained as the byproduct from shrimp processing, low in cost and low in molecular weight, can rapidly enter blood circulation to be absorbed and utilized by a human body, has good antioxidant effect, high safety and no toxic or side effects, and is a top-quality antioxidant peptide provided for the fields of food, cosmetic and medicine..

Description

technical field [0001] The invention relates to a method for preparing antioxidant peptides from shrimp shells, belonging to the field of biotechnology. Background technique [0002] With the development of scientific research, especially the proposal of free radical theory, human beings have a deeper understanding of the aging of the body, and realize that excessive free radicals can not only lead to aging of the body, but also lead to various diseases. Using antioxidant substances to eliminate free radicals can delay aging, enhance immunity, and prevent the occurrence of diseases. Therefore, antioxidant substances have become a research focus of researchers from various countries in recent years. [0003] Antioxidant peptides are one of the major categories of antioxidant substances. Usually, biologically active peptides that have the function of inhibiting the peroxidation of biological macromolecules or eliminating the body's freedom and function are called antioxidant ...

Claims

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Application Information

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IPC IPC(8): C12P21/06
Inventor 罗自生施旭丹王延圣
Owner ZHEJIANG UNIV
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