Pilot scale astragalus residue cordycepin solid state fermentation method
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A technology of solid fermentation and Cordyceps fungus, which is applied in the field of biotechnology applications, can solve the problem of secondary utilization of astragalus slag, and achieve the effect of reducing crude fiber content and pollution
Inactive Publication Date: 2012-10-10
TIANJIN UNIV OF SCI & TECH
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In addition, the application number is 200910114886.1, and the patent is named "A Feed Additive of Fungal Fermentation and Its Preparation Method and Application". High-yield strains are used as strains to perform solid-state fermentation on corn flour and soybean meal as solid-state fermentation substrates to obtain Paecilomyces hematodes fermentation product. Although the strains used in this invention are fungi, they are not an invention of secondary utilization of astragalus dregs
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[0023] 1. The bacterial classification Paecilomyces sinensis used in the present invention is inoculated on a PDA slant, cultivated at a constant temperature at 20° C. for 7 days, and after maturation, make a spore suspension with sterile water for use.
[0024] Described PDA slant culture medium is potato culture medium, and its specific preparation method is: peeled potato 200g is cut into fritters, adds water 500mL and boils 30min, after 40% gauze is filtered, adds glucose 20g in filtered juice, agar 20g and heats, After the agar is completely dissolved, add water to 1000mL, aliquot, sterilize, and set aside.
[0025] 2. Sterilize the seed medium by 0.1Mp, 121°C, 20-30min. After cooling, add 1-5% of the spore suspension in step 1, shake well, and cultivate in a 20°C, 150r / min constant temperature shaking incubator for 3 days, as a first-level seed liquid, for inoculation into a solid substra...
[0032] 1. Cultivate 20 shallow dishes according to the cultivation method of Example 1. It takes 4 days from the preparation of the seed solution to the completion of the inoculation. Use 6000 g of astragalus dregs and cultivate for 38 days. After fermentation, 4200 g of the fermentation product is obtained, and the product yield is 70%.
[0033] 2. Cultivate 40 shallow dishes according to the cultivation method of Example 1. It takes 4 days from the preparation of the seed solution to the completion of the inoculation. Use 12000 g of astragalus dregs and cultivate for 38 days. After fermentation, 8400 g of the fermentation product is obtained, and the product yield is 70%.
[0034] 3. Cultivate 80 shallow dishes according to the cultivation method of Example 1. It takes 4 days from the preparation of the seed solution to the completion of the inoculation. Use 24000 g of astragalus dregs and cultiv...
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Abstract
The invention relates to the field of application of biotechnology and belongs to utilization of traditional Chinese medicine residues. After astragalus polyose is extracted industrially, the remaining astragalus residue can serve as a matrix of Chinese paecilomyces mycelium solid state fermentation. The invention provides a method for pilot scale amplification culture of the solid state fermentation process. The pilot scale amplification culture method for an astragalus residue cordycepin solid state fermentation process adopts a shallow tray fermentation method, wherein the outline of a shallow tray is of a rectangle; the shallow tray is 280 mm long, 180 mm wide and 120 mm high, is made of high-temperature resisting polypropylene material, and is provided with a sterile vent. The method is characterized in that during fermentation, leavening can be placed in the shallow tray, the shallow tray is placed in a plastic bag made of polypropylene material, a cotton tampon is used to seal the vent, and then sterilization and inoculation can be carried out. With increase of manual labor, the method can fully amplify culture scale, can make cyclic culture possible, and finally makes industrial production possible; and astragalus residue cordycepin baking powder with stable property can be obtained.
Description
technical field [0001] The invention relates to the field of biotechnology application, and belongs to the utilization of traditional Chinese medicine dregs. The astragalus dregs left after extracting astragalus polysaccharides through "water extraction and alcohol precipitation" are used as the substrate for solid-state fermentation of Paecilomyces chinensis mycelium, and the solid-state fermentation process is carried out. Pilot-scale scale-up culture method. Background technique [0002] Traditional Chinese medicine residue is a large amount of residues containing various residual components left after the medicinal materials are processed into finished medicines. It contains many active ingredients that can be used, but most of them are discarded, which brings great pressure to the environment. With the enhancement of people's awareness of environmental protection and the development of science and technology, more and more researches are devoted to solving the residual ...
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