Pilot scale astragalus residue cordycepin solid state fermentation method

Abstract

The invention relates to the field of application of biotechnology and belongs to utilization of traditional Chinese medicine residues. After astragalus polyose is extracted industrially, the remaining astragalus residue can serve as a matrix of Chinese paecilomyces mycelium solid state fermentation. The invention provides a method for pilot scale amplification culture of the solid state fermentation process. The pilot scale amplification culture method for an astragalus residue cordycepin solid state fermentation process adopts a shallow tray fermentation method, wherein the outline of a shallow tray is of a rectangle; the shallow tray is 280 mm long, 180 mm wide and 120 mm high, is made of high-temperature resisting polypropylene material, and is provided with a sterile vent. The method is characterized in that during fermentation, leavening can be placed in the shallow tray, the shallow tray is placed in a plastic bag made of polypropylene material, a cotton tampon is used to seal the vent, and then sterilization and inoculation can be carried out. With increase of manual labor, the method can fully amplify culture scale, can make cyclic culture possible, and finally makes industrial production possible; and astragalus residue cordycepin baking powder with stable property can be obtained.

Description

technical field

[0001] The invention relates to the field of biotechnology application, and belongs to the utilization of traditional Chinese medicine dregs. The astragalus dregs left after extracting astragalus polysaccharides through "water extraction and alcohol precipitation" are used as the substrate for solid-state fermentation of Paecilomyces chinensis mycelium, and the solid-state fermentation process is carried out. Pilot-scale scale-up culture method. Background technique

[0002] Traditional Chinese medicine residue is a large amount of residues containing various residual components left after the medicinal materials are processed into finished medicines. It contains many active ingredients that can be used, but most of them are discarded, which brings great pressure to the environment. With the enhancement of people's awareness of environmental protection and the development of science and technology, more and more researches are devoted to solving the residual ...