In vitro amplification method of self-specific T cell, prepared T cell system, pharmaceutical use of cell system and component monitoring method of cell system

Abstract

The invention relates to an in vitro amplification method of self-specific T cells, a T cell system prepared by the method, pharmaceutical use of the cell system, and a component monitoring method of the cell system. The in vitro amplification method comprises the steps of: (1) separating peripheral blood mononuclear cells from peripheral blood of a patient; (2) adding corresponding T cell stimulating factors as needed, adding or not adding corresponding cell growth factors, and amplifying T cells by cultivating the peripheral blood mononuclear cells separated in the step (1) in a corresponding culture medium. According to the invention, polyclonal immunocompetent cells with various functionalities can be prepared according to different clinical needs by adopting the amplification method of the invention in a GMP environment, and the prepared polyclonal immunocompetent cells can be used in immune system regeneration and immunotherapy of patients.

Description

technical field [0001] The present invention relates to an in vitro expansion method of autologous specific T cells, and also relates to the autologous specific T cell system obtained after the expansion by the method, the application of the cell system in medicine preparation and the components of the cell system monitoring method. Background technique [0002] Tumor treatment has been using three traditional treatment methods, namely radiotherapy, chemotherapy and surgery. Traditional surgical treatment can only solve local problems. Radiotherapy and chemotherapy can also damage normal tissues and cells of the human body while killing cancer cells, especially the immune function of T cells that dominate cellular immunity. tumor. Therefore, the three traditional treatment methods of radiotherapy, chemotherapy and surgery have always had disadvantages such as incompleteness, easy transfer, and large side effects. [0003] In recent years, a new tumor autologous cell immun...

AI Technical Summary

Problems solved by technology

[0006] 1. The in vitro cell culture system for autologous cells is not perfect, and there are many uncontrollable factors, which lead to insufficient or failed expansion of cells, and more importantly, the cultured cells lack the specificity of killing tumors;

[0007] 2. There is a lack of qua

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