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Culture medium and method for producing naringinase by fermenting citrus peel powder and beam dregs by aspergillus niger

A technology for fermentation and culturing of Aspergillus niger, which is applied in the field of culture medium for producing naringinase, can solve the problems of less research on naringinase, and achieve the effects of increasing yield, realizing high-value utilization, and reducing environmental pollution.

Inactive Publication Date: 2012-10-17
FUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Scholars at home and abroad have extensively and in-depth research on the metabolic pathway of limoninase and its debittering pathway, but less research on naringinase

Method used

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  • Culture medium and method for producing naringinase by fermenting citrus peel powder and beam dregs by aspergillus niger
  • Culture medium and method for producing naringinase by fermenting citrus peel powder and beam dregs by aspergillus niger
  • Culture medium and method for producing naringinase by fermenting citrus peel powder and beam dregs by aspergillus niger

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Embodiment Construction

[0022] The following uses Aspergillus niger S-05 strain (Reference: Tan Xiaofang et al., Purification and isolation of naringinase produced by Aspergillus niger S-05. Food and Fermentation Industry, 2010, 36(8): 59-63.) for production strain, and its enzyme production medium and fermentation conditions were optimized.

[0023] 1 Determination method of naringinase activity

[0024] The enzyme activity was determined by Davis method (modified method). Mix 2.0 mL of 0.2 mol / L acetate buffer (pH 4.0) with 2.0 mL of 400 μg / mL naringin standard solution, and keep at 40 °C for 5 min; add 1.0 mL of appropriately diluted crude enzyme solution (the The fermentation broth obtained by liquid fermentation was centrifuged at 4 °C and 8000 r / min for 10 min, and the supernatant was collected as crude enzyme solution), and reacted in a water bath at 40 °C for 30 min; 0.5 mL of reaction solution and 2.5 mL of naringin color developing solution ( 90% diethylene glycol: 1mol / L NaOH solution = ...

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Abstract

The invention belongs to the field of bio-manufacturing and particularly relates to a culture medium and method for producing naringinase by fermenting citrus peel powder and beam dregs by aspergillus niger. The culture medium is prepared from 2.0-3.0% of citrus peel powder, 0.5-2.5% of bean dregs, 0.2% of yeast extract 0.2%, 1.0% of KH2PO4, 0.1% of Na2HPO4, 1.0-2.5mmol / L MgSO4.7H2O, 0.5-1.0 mmol / L FeCl3 and 1.0-5.0 mmol / L CaCl2. The initial pH value of the culture medium is 5.0-6.0, and the culture condition is as follows: the inoculum size is 5.0-9.0%, the culture temperature is 30-33 DEG C, the liquid filling amount is 30-50mL / 250mL, and the rotating speed of a shaking bed is 180r / min. Due to the adoption of the culture medium and the method, the yield of the naringinase is increased, and a foundation is laid for the production and the application of the naringinase.

Description

technical field [0001] The invention belongs to the field of biological manufacturing, and more specifically relates to a medium and a method for producing naringinase by using Aspergillus niger to ferment orange peel powder and bean dregs. Background technique [0002] Because citrus fruit juice is rich in nutrients and has health benefits, it has become a fruit juice product with a large consumption and occupies about 67% of the world market. At present, the amount of domestically produced citrus fruits used for processing is only 5% of the total, which makes the development of the domestic fruit juice processing industry lag behind. The orange juice sold in the international market mainly comes from Brazil and the United States, of which the United States mainly focuses on the production of grapefruit juice. Grapefruit (Grapefruit), also known as grapefruit, originated in Asia and is mainly produced in Asia, Africa and Latin America. Grapefruit is very similar to pomelo...

Claims

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Application Information

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IPC IPC(8): C12N9/24C12R1/685
Inventor 叶秀云林娟陈增蔡梅华杨捷
Owner FUZHOU UNIV
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