Probiotic goat milk tablet and preparation method of probiotic goat milk tablet
A technology of probiotics and goat milk tablets, which is applied in the field of probiotic goat milk tablets and its preparation, can solve the problems of single species of probiotics, and achieve the effects of promoting proliferation, inhibiting growth, and lowering cholesterol
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Embodiment 1
[0027] 1) First, the probiotics Lactobacillus acidophilus and Lactobacillus plantarum were respectively activated at 37°C using MRS liquid medium for three times to obtain activated probiotics. % of the inoculum was inserted into a fermenter of 10L MRS medium and cultivated for 18h, stirred every 3h during the cultivation process, the rotating speed was 60 rev / min, the stirring time was 2min, and then centrifuged for 12min at a rotating speed of 8000 rpm / min with a centrifuge Make probiotic bacteria mud, then add 1 mL of compound freeze-drying protective agent per gram of probiotic bacteria mud to the probiotic bacteria mud, mix well with a vortex mixer, pre-freeze at -40 °C for 12 hours, and then move it to a freeze dryer Freeze-dried for 24h to obtain freeze-dried probiotic bacteria powder;
[0028] The composite freeze-drying protective agent is composed of 18% skimmed milk powder, 20% trehalose, 2% disodium hydrogen phosphate, 10% lactose and 50% phosphate buffer by mass p...
Embodiment 2
[0032] 1) First, the probiotics Lactobacillus acidophilus and Lactobacillus rhamnosus were activated three times in MRS liquid medium at 37°C respectively to obtain activated probiotics, and the activated Lactobacillus acidophilus and Lactobacillus rhamnosus were obtained. The fermenter with 5% inoculum access to 10L MRS medium was cultivated for 18h, stirred every 4h during the cultivation process, the rotating speed was 60 rev / min, the stirring time was 2 min, and then, the centrifuge was used at a rotating speed of 8000 rpm∕min. Centrifuge for 12 minutes to make probiotic bacteria mud, then add 1 mL of compound freeze-drying protective agent per gram of probiotic bacteria mud to the probiotic bacteria mud, mix well with a vortex mixer, pre-freeze at -30 °C for 18 hours, and then move to the freezer Freeze-dried in a dryer for 24 hours to obtain freeze-dried probiotic bacteria powder;
[0033] The composite freeze-drying protective agent is composed of 50% skimmed milk powde...
Embodiment 3
[0037] 1) First, the probiotic Lactobacillus acidophilus was activated three times in MRS liquid medium at 37°C to obtain activated Lactobacillus acidophilus, and then Bifidobacterium infantis was added to the MRS liquid medium with 0.05% of the medium volume. Cysteine hydrochloride was activated three times in MRS liquid medium at 37°C, and the inoculum of 5% was transferred each time. The first two were incubated at 37°C for 20 h, and the third time Incubate at 37°C for 18 hours, then carry out microscopic examination and measure the OD value to determine the absence of impurities, and activate the activated Bifidobacterium infantis for the third time. Take the activated probiotics Lactobacillus acidophilus and Bifidobacterium infantis 10L MRS medium (add 5g of cysteine hydrochloride in MRS medium) with 5% inoculum was cultured for 18h, stirred every 4h during the culture, and the rotation speed was 60 rev / min. The stirring time is 2min, then, centrifuge at 8000 rpm for ...
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