Paddy biofertilizer, preparation method thereof and bacillus subtilis

A technology of Bacillus subtilis and bio-fertilizer, applied in the field of agriculture, can solve the problems of not being able to prove the good growth of rice, not being able to improve the growth of rice, and increasing the root weight, so as to advance the rice tillering period, increase the number of rice tillers, and increase the root weight Effect

Active Publication Date: 2012-10-31
JILIN LIZE BIOLOGICAL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are many special fertilizers for rice on the market, but most of them are inorganic compound fertilizers or organic-inorganic compound fertilizers, that is, fertilizers that produce nitrogen, phosphorus, potassium or nitrogen, phosphorus, potassium and organic matter in a certain ratio. Fertilizers only provide the nitrogen, phosphorus, and potassium nutrients needed by rice, and they cannot make rice grow well in low temperature environments, such as increasing the number of tillers, advancing the tillering period, increasing root weight, deepening leaf color, and increasing rice water temperature, etc.
[0005] The Chinese patent application No. 200810137386.5 discloses "a new type of special microbial organic fertilizer production process for rice", which mainly improves the activity of microorganisms by improving the carrier for adsorbing microorganisms, so that microorganisms can efficiently decompose nitrogen, phosphorus and potassium for rice to absorb. However, it is not conducive to the growth of rice under cold stress, and there is no relevant experimental data to prove
[0006] Chinese patent application No. 201010595301.5 discloses "a special microbial waste material rich in sil

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: Screening of Bacillus subtilis HY01 of the present invention

[0036] Take the commercially available wild-type Bacillus subtilis as the starting strain, culture it with a nutrient solution containing a mixture of rare earths at 30°C and 120r / min for 12 hours, then select a strong strain and repeat the above-mentioned cultivation steps 3-5 times, and then select a strong strain Bacterial strains are subjected to ordinary culture, when the number of bacteria reaches 10 7 cells / ml, treated under 500W microwave for 30-45s, screened the most robust strain, and named it HY01. Wherein, the nutrient solution is composed of 100 parts of beef extract, 1 part of peptone, 0.5 part of sodium chloride, and 0.15 part of rare earth mixture in parts by weight (gadolinium chloride: cerium nitrate weight ratio is 2:1), pH7. 2-7.4.

[0037] The above-screened strain HY01 was tested for its physiological and biochemical characteristics according to the method of "Common Bac...

Embodiment 2

[0038] Embodiment 2: the decomposing fermentation of poultry manure

[0039] Livestock manure: cow manure, deer manure, pig manure, chicken manure;

[0040] In terms of mass percentage, mix 70% poultry manure, 20% forage or straw powder, and 10% bran and place them in a fermentation tank. The height of the mixed material is 0.5-1.2 meters. When the temperature of the fermented material rises to 60 ° C, Start to turn the compost, and control the temperature not to exceed 70°C. The number of turns depends on the temperature of the fermented material. The fermentation process is generally completed in 4-6 days. The fermented material is fluffy, dark brown, and slightly fragrant or earthy.

Embodiment 3

[0041] Embodiment 3: prepare rice biological fertilizer of the present invention

[0042] Solid material composition: 40 parts of rice husk powder, 50 parts of wheat bran, 5 parts of soybean cake powder, 5 parts of corn flour, 0.05 part of magnesium sulfate and 0.01 part of manganese sulfate, the ratio of material to water is 1:1, tunnel microwave sterilization , adjust the pH value to 7.0-7.2 with alkaline substances such as calcium hydroxide;

[0043] Adding Bacillus subtilis with preservation number CGMCC No.6190 to the solid material and fermenting until the number of Bacillus subtilis is at least 1.2×10 10 per g, and the rate of Bacillus subtilis spores reaches at least 60%, and the rate of miscellaneous bacteria is less than 20%, to obtain fermented solid materials;

[0044] Take 5 parts of fermented solid material, 7.7 parts of humic acid, 0.3 part of agricultural rare earth (REO ≥ 40%), 50 parts of poultry manure of embodiment 2, silicon calcium magnesium fertilizer (...

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PUM

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Abstract

The invention relates to the technical field of agriculture, and discloses a paddy biofertilizer, a preparation method thereof and bacillus subtilis. The preparation method comprises the following steps of: adding bacillus subtilis with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.6190 into a solid material, fermenting until the quantity of bacillus subtilis is at least 1.2*10<10>/g, the spore percentage reaches at least 60% and the impurity microorganism percentage is less than 20%, and then uniformly mixing the fermented solid material with humic acid, agricultural rare earth, aged and fermented poultry and livestock feces, silicon-calcium-magnesium fertilizer and amino acid fertilizer so as to obtain the paddy biofertilizer. According to the invention, the mutagenic bacillus subtilis is mixed with chicken manure and other aged and fermented organic matters, humic acid, silicon-calcium-magnesium fertilizer, agricultural rare earth, amino acid fertilizer and the other substances to form the compound paddy microbe manure, so that the number of tillers of the paddy is increased, the tillering stage of the paddy is advanced, the root weight of the paddy is increased, the leaf color of the paddy is enhanced, the water temperature is raised, and the growth of the paddy under the cold stress is promoted.

Description

technical field [0001] The invention relates to the technical field of agriculture, in particular to a rice biological fertilizer, a preparation method thereof and bacillus subtilis. Background technique [0002] Black soil refers to a special soil with an average organic matter content between 3% and 10%, which is especially conducive to the growth of rice, wheat, soybeans, corn and other crops. Because the black soil is distributed in the cold temperate zone with four distinct seasons, the vegetation is lush, and the change of cold and hot seasons causes a large number of dead branches and leaves to rot and decompose, forming a thick and fertile black soil layer. The name "cold black soil" comes from this. . At present, the products of the cold black soil have become the representative of the best quality of green organic food in the world. [0003] However, low temperature and cold damage in cold regions is a serious natural disaster in agricultural production, involvin...

Claims

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Application Information

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IPC IPC(8): C05G1/00C05F17/00C12N1/20C12R1/125
CPCY02W30/40
Inventor 赵立春邹建国丁振生
Owner JILIN LIZE BIOLOGICAL SCI & TECH CO LTD
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