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Small RNA used for regulating expression of B7-H2 gene

A technology of B7-H2 and gene expression, applied in the field of regulation of B7-H2 gene expression and microRNA, can solve the problems of different functions, difficulty of miRNA, and no correlation between miR-130b and B7-H2 gene

Inactive Publication Date: 2012-11-21
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007]Although it is known in the art that certain microRNAs are related to tumors, there are many kinds of miRNAs known in the art with different functions. It is difficult to screen out specific miRNAs that are related to tumors and can be used as pathogenesis, treatment options and prognosis
Currently, there is no report on the correlation between miR-130b and B7-H2 gene in this field

Method used

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  • Small RNA used for regulating expression of B7-H2 gene
  • Small RNA used for regulating expression of B7-H2 gene
  • Small RNA used for regulating expression of B7-H2 gene

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Embodiment Construction

[0026] The above solution will be further described below in conjunction with specific embodiments. It should be understood that these examples are used to illustrate the present invention and not to limit the scope of the present invention. The implementation conditions used in the examples can be further adjusted according to the conditions of specific manufacturers, and the implementation conditions not indicated are usually the conditions in routine experiments.

[0027] 1. Reagents and materials

[0028] 1. Reagents

[0029] Fetal bovine serum (Hyclone, USA); complete medium: add 100ml of fetal bovine serum, 0.15g of L-glutamine, 10.0ml of 2-mercaptoethanol (5×10 -3 mol / L); liposome 2000 (Invitrogen, USA); penicillin, streptomycin (Shanghai Sangon Biotechnology Co., Ltd); TRIzol (Invitrogen, USA); agarose (LP0028A, Oxoid Ltd, UK); gel Electrophoresis sample solution and ethidium bromide (EtBr) (Shanghai Sangon Biotechnology Co., Ltd.); gel recovery DNA kit and...

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Abstract

The invention discloses a small RNA used for regulating expression of the B7-H2 gene. The small RNA is nucleic acid or a functional fragment or variant comprising the following sequence: 5'-cagugcaaugaugaaagggcau-3' and is miR-130b. According to results of in vitro biological function experiments carried out by the inventor, hsa-miR-130b can bind with 3'-UTR of the B7-H2 gene, thereby inhibiting expression of B7-H2 molecules; and an antitumor effect is exerted through regulation the expression and functions of B7-H2 signaling pathways and / or miR-130b.

Description

technical field [0001] The invention belongs to the field of biotechnology and medical technology, specifically, the invention relates to the technical field of gene expression regulation, in particular to a kind of microRNA used to regulate the expression of B7-H2 gene. Background technique [0002] Tumor has been a serious threat to human health and life, and traditional treatment methods are not satisfactory. Immunotherapy achieves the purpose of treating tumors by improving the immunogenicity of tumors and inducing the body to produce specific anti-tumor immune responses. It is a biological treatment method that changes from passive to active. Cellular immunity mediated by T cells is the main form of anti-tumor immunity. Through the specific recognition of tumor antigens, the body activates T cells and produces corresponding immune killing effects. [0003] It has been demonstrated that the efficient activation and function mediation of T cells requires the synergy of d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113
Inventor 汪维鹏朱健洁张学光
Owner SUZHOU UNIV
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