Bacterial strain capable of secreting cellulase as well as cellulase extraction method and application thereof

A cellulase and high cellulose technology, applied in the biological field, can solve the problems of low enzyme activity and yield, and achieve the effects of high cellulose degradation activity, wide industrial application prospects, and wide sources.
CN102807958AInactive Publication Date: 2012-12-05WUHAN UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
WUHAN UNIV OF SCI & TECH
Publication Date
2012-12-05
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a bacterial strain capable of secreting cellulase as well as the cellulase extraction method and application thereof, and solves the problems of low enzyme activity and yield in the conventional cellulase producing bacterial strain. The bacterial strain has a collection name of trichoderma virens ZY-01, is collected in China Center For Type Culture Collection (CCTCC), has a collection number of CCTCC No: M 2012205 and has an 18s rDNA sequence as shown in SEQ ID NO.1. A method for separating the bacterial strain, a method for producing, purifying and extracting the cellulase and an application method for efficient degradation of bagasses and straws are described. The bacterial strain has the advantages of high enzyme activity, wide range of adapted reaction condition and simplification of preparation; and the secreted cellulase is high in cellulose degradation activity.
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Description

technical field

[0001] The invention relates to the biological field, in particular to a method for isolating a bacterial strain capable of secreting cellulase, the cellulase produced by the bacterial strain and its extraction method and application. Background technique

[0002] Cellulose is the most widely distributed polysaccharide in nature. If it can be rationally utilized and degraded into usable sugars, it can solve the current food and energy problems faced by human society. Cellulase is the key enzyme for hydrolyzing cellulose into fermentable sugars. After cellulose is hydrolyzed, reducing sugars can be obtained, which can be further converted into bioenergy and other substances through fermentation processes. Adding cellulase to forage can increase the utilization rate of feed and increase the meat production rate of animals. At present, cellulase is also widely used in industrial fields such as textile and papermaking.

[0003] Most of the fungi used to produc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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