Primer and kit for detecting coxsackievirus A6 type RT-LAMP (Reverse Transcription Loop-mediated Isothermal Amplification) nucleic acid

Abstract

The invention relates to the application field of biological detection technologies, and in particular relates to a primer pair and a kit for coxsackievirus A6 type RT-LAMP (Reverse Transcription Loop-mediated Isothermal Amplification) nucleic acid detection. The primer pair comprises two outer primers F3 and B3, two inner primers FIP and BIP and two loop primers LF and LB; and the kit comprises the primer pair. The CA6RT-LAMP has the characteristics of strong specificity, high sensitivity, simplicity in operation methods, quick detection, easiness in result judgment, low cost and the like, can better satisfy the requirement of on-site quick detection, is easy to popularize and apply in grassroots units, can be used for early rapid diagnosis of epidemic outbreaks, such as hand-foot-and-mouth diseases and the like and clinical cases in disease prevention and control organizations, hospitals, kindergarten units, and has broad market prospect and enormous economic and social benefits.

Description

technical field [0001] The invention relates to the application field of biological detection technology, in particular to primers and kits for nucleic acid detection of Coxsackievirus A6 (Coxsackievirus A6, CA6 for short) various specimens (feces, anal swab, throat swab, herpes fluid, cerebrospinal fluid). Background technique [0002] Coxsackievirus A6 is a single-stranded positive-sense RNA virus belonging to the Picoranviridae family. CA6 can cause diseases such as hand, foot and mouth disease and herpetic angina. Although CA6 is generally not considered to be the main pathogen of HFMD, there have been reports of outbreaks of HFMD caused by CA6 in countries and regions such as Singapore, Finland, Taiwan, and Japan in recent years, and HFMD caused by CA6 is related to other types of intestinal Enteroviruses caused by enteroviruses are more likely to cause symptoms such as Beau's lines and nail shedding. The mechanism is unclear, but it may indicate that CA6 has a wider s...

AI Technical Summary

Problems solved by technology

At present, the classic methods for detecting enteroviruses mostly use cell separation and culture and specific antibody detection. This method has cumbersome steps and a long detection cycle, and some enteroviruses are difficult to proliferate in cells, and are prone to missed detection; based on CA6 Nucleic acid amplification molecular biology diagnostic techniques such as PCR, RT-PCR, and real-time fluorescent quantitative RT-PCR (real-time fluorescent RT-PCR) play an important role in the detection of pathogenic microorganisms and disease diagnosis, but these methods or It requires precise temperature control equipment and advanced and complex analytical instruments, or requires relatively high proficiency and professional level of operators, and the reaction time is long, which is not conducive to the detection of on-site samples, is not conducive to the promotion at the grassroots level, and cannot meet the requirements of simple, fast, Accurate diagnosis is required; the key to the prevention, control and treatment of the epidemic lies in the rapid, accurate and early detection and diagnosis of pathogenic microorganisms

Images