Method and device for researching bio-availability of dissoluble organic nitrogen in water body

Abstract

The invention provides a device for researching bio-availability of dissoluble organic nitrogen in water body. A main body of a filter device is a container, a hollow fiber microfiltration membrane assembly is filled inside the container, a micropore aeration disc is installed below the membrane assembly, a bottom of a reactor is conical, and a spoil disposal port is arranged at the bottom of the reactor. Another end of the hollow fiber microfiltration membrane assembly is connected with a second water collecting tank. A main body of a dissoluble organic nitrogen extraction device is a cross flow filtration device, a water inlet of the dissoluble organic nitrogen extraction device is connected with the second water collecting tank, a concentrated solution outlet of the cross flow filtration device is connected with the second water collecting tank, and a penetrating fluid outlet is connected with a third water collecting tank. A main body of an algae growth system is a reaction tank, a water inlet of the reaction tank is connected with the second water collecting tank, a temperature sensor and a heating rod are arranged in the reaction tank, and a light source capable of adjusting luminance and a light sensor are installed on the top of the reaction tank. A circulating pump is installed outside of the reaction tank to pump water at the bottom of the reaction tank to a water surface. The invention further discloses a method for researching the bio-availability of the dissoluble organic nitrogen in the water body by using the device.

Description

Technical field

[0001] The invention belongs to the field of aquatic ecology research, and specifically relates to a device for studying the bioavailability of dissolved organic nitrogen in a water body.

[0002] The present invention also relates to a method for studying the bioavailability of soluble organic nitrogen in water by using the above-mentioned device. Background technique

[0003] Dissolved organic nitrogen (DON) is the main component of soluble total nitrogen in freshwater bodies such as rivers, lakes, wetlands, etc. The average can reach more than 60%, of which 12-72% can be directly used by organisms. The vast majority of DON comes from land infiltration and surface runoff, especially DON that is transported to estuaries and lakes by man is an important source of freshwater bodies. In order to control the eutrophication process of lakes, many sewage treatment plants have added denitrification processes, and inorganic nitrogen has been successfully removed. However,...

Examples

Embodiment Construction

[0029] The device for studying the bioavailability of dissolved organic nitrogen in the water body provided by the present invention is composed of a filter device, a dissolved organic nitrogen extraction device and an algae growth system. The main process includes:

[0030] 1) A hollow fiber microfiltration membrane module and a microporous aeration plate are installed in the filter device to remove particles and plankton with a pore size greater than 0.45μm;

[0031] 2) The filtered water sample enters the dissolved organic nitrogen extraction device, which is equipped with a cross-flow filtration device. Any ultrafiltration membrane with a molecular weight cut-off of 1k-100kDa or a nanofiltration membrane with a molecular weight cut-off of 170-300Da is used for error. Flow filtration, so that DON of different molecular weight is concentrated;

[0032] 3) The concentrated water sample enters the algae growth system, which includes DON continuous input device, temperature and light...

Technical field

[0001] The invention belongs to the field of aquatic ecology research, and specifically relates to a device for studying the bioavailability of dissolved organic nitrogen in a water body.

[0002] The present invention also relates to a method for studying the bioavailability of soluble organic nitrogen in water by using the above-mentioned device. Background technique

[0003] Dissolved organic nitrogen (DON) is the main component of soluble total nitrogen in freshwater bodies such as rivers, lakes, wetlands, etc. The average can reach more than 60%, of which 12-72% can be directly used by organisms. The vast majority of DON comes from land infiltration and surface runoff, especially DON that is transported to estuaries and lakes by man is an important source of freshwater bodies. In order to control the eutrophication process of lakes, many sewage treatment plants have added denitrification processes, and inorganic nitrogen has been successfully removed. However,...

Examples

Embodiment Construction

[0029] The device for studying the bioavailability of dissolved organic nitrogen in the water body provided by the present invention is composed of a filter device, a dissolved organic nitrogen extraction device and an algae growth system. The main process includes:

[0030] 1) A hollow fiber microfiltration membrane module and a microporous aeration plate are installed in the filter device to remove particles and plankton with a pore size greater than 0.45μm;

[0031] 2) The filtered water sample enters the dissolved organic nitrogen extraction device, which is equipped with a cross-flow filtration device. Any ultrafiltration membrane with a molecular weight cut-off of 1k-100kDa or a nanofiltration membrane with a molecular weight cut-off of 170-300Da is used for error. Flow filtration, so that DON of different molecular weight is concentrated;

[0032] 3) The concentrated water sample enters the algae growth system, which includes DON continuous input device, temperature and light...

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