Method for extracting betulinic acid from persimmon leaves

Abstract

The invention discloses a method for extracting betulinic acid from persimmon leaves. The method comprises the steps that dry persimmon leaves are taken to be ground, added with water to mix and wet, added with bio-enzyme for natural enzymolysis for 4-7 days, the enzymolysis raw material is added with 70-90% ethanol and extracted under refluxing for 1-3 times, the extract is concentrated under reduced pressure until the alcohol concentration is 50-60%, and stood for crystallization, crystal substances are dissolved with 5-7 times of amount of alkaline ethanol solution, undissolved substances are filtered out, filtrate is regulated through hydrochloric acid or sulfuric acid solution to be neutral and centrifuged, crude extract is dissolved with ethanol for recrystallizing, and the betulinic acid is obtained after drying. By adopting the method for extracting the betulinic acid, the raw material resources are abundant, the method is simple to operate, and the industrialization production is benefited.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for extracting betulinic acid from persimmon leaves. Background technique [0002] Betulinic acid, also known as betulinic acid, has a CAS number of 472-15-1 and a molecular formula of C 30 h 48 o 3 , the molecular weight is 456.71, and the molecular structure formula is: [0003] . [0004] Betulinic acid is a pentacyclic triterpenoid compound, insoluble in water and petroleum ether, slightly soluble in methanol, ethanol, acetone, easily soluble in tetrahydrofuran and pyridine. Betulinic acid can selectively kill human melanoma cells without killing healthy cells, and it also has inhibitory effect on HIV-1 infection. In addition, recent studies have shown that betulinic acid also has inhibitory effects on malignant tumor cells such as brain tumors, neuroectodermal tumors, and leukemia. [0005] There are currently three main sources of betulinic acid: first, it is e...

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Problems solved by technology

[0007] As mentioned above, the existing methods for producing betulinic acid are relatively cumbersome, and there are many types of reagents, especially the macroporous resin and polyamide resin are difficult to handle, and there are many elution solvents