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Plant growth-promoting rhizobacterium SXH-3 and application thereof

A technology of SXH-3, rhizosphere growth-promoting bacteria, applied in the direction of application, bacteria, biocide, etc., can solve the problems of reduced utilization rate, damage to the soil environment, and limited yield of leeks, so as to improve stress resistance and reduce massive accumulation , the effect of promoting the absorption of phosphorus

Inactive Publication Date: 2014-04-16
HARBIN NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, planting leeks requires a large amount of chemical fertilizers, which not only affects the quality of leeks, but also damages the soil environment, and it is a well-known fact that with the extensive use of chemical fertilizers, their utilization rate continues to decrease
This shows that it is limited to increase the yield of leeks only by increasing the application of chemical fertilizers in large quantities.

Method used

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  • Plant growth-promoting rhizobacterium SXH-3 and application thereof
  • Plant growth-promoting rhizobacterium SXH-3 and application thereof
  • Plant growth-promoting rhizobacterium SXH-3 and application thereof

Examples

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Effect test

Embodiment 1

[0038] Example 1. Isolation and Identification of Klebsiella oxytoca SXH-3

[0039] 1. Isolation of Klebsiella oxytoca SXH-3

[0040] The isolation of Klebsiella oxytoca SXH-3 includes two steps of sampling, screening and purification, the specific method is as follows:

[0041] 1.1. Sampling

[0042] Screening is carried out from the rhizosphere soil of Chinese chives, and the specific chives and soil for testing are taken from Shuangjing Town, Hulan District, Harbin City, Heilongjiang Province (the physical and chemical properties of the soil are as follows: pH 8.15, total salt content 1.83%, alkali-hydrolyzable nitrogen 36mg / kg , available phosphorus 8mg / kg, available phosphorus 132mg / kg, organic matter 2.69%). Put leeks and plant rhizosphere soil into clean fresh-keeping bags prepared in advance, and bring them back to the laboratory for planting for testing.

[0043] Weigh 1g of rhizosphere soil sample into a Erlenmeyer flask containing 50mL of PAF culture solution, an...

Embodiment 2

[0064] The ACC deaminase activity determination of embodiment 2, CGMCC No.6298

[0065] The tested strain was cultured overnight in TSB culture solution (tryptone 17.0g, soytone 3.0g, NaCl 5.0g, glucose 2.5g, K2HPO 42.5g, distilled water 1000mL, pH 7.5), and the bacteria were collected by centrifugation at 4°C. The bacterial cells were washed three times with DF medium, resuspended in ADF medium, cultured with shaking at room temperature (21±1°C) for 2 days, collected by centrifugation at 4°C, and washed with 0.1mol·L -1 Wash and centrifuge three times with Tris-HCl buffer (pH=7.6), resuspend in 600 μL 0.1mol L -1 In Tris-HCl buffer (pH=8.0), add 30 μL of toluene and shake rapidly for 30 s to break the cells, transfer 200 μL of toluene-containing cell extract to a 1.5 mL centrifuge tube, add 20 μL of 0.5 mol·L -1 Mix ACC evenly, and do a blank test without adding ACC at the same time, and incubate at 30°C for 15 minutes. Add 1mL 0.56mol·L -1 HCl, centrifuge at 16000g for 5m...

Embodiment 3

[0070] Embodiment 3, the mensuration of CGMCC NO.6298 somatotropin IAA synthesis content

[0071] The test strain Klebsiella oxytoca (Klebsiella oxytoca) SXH-3 was first cultured in DF culture medium for 2 days, and then transferred into DF culture medium with different concentrations of tryptophan (L-Trp) (containing 0, 50, 100, 200 and 500 μg L-Trp mL -1 ) to continue culturing for 2 days, and take a sample to test the OD of the bacterial solution 600 , centrifuge the remaining culture solution at 8000 g at room temperature, take 500 μL supernatant, add 2 mL Salkowski reagent (containing 150 mL H 2 SO 4 , 250mL ddH 2 O and 7.5mL 0.5mol / L FeCl 3 ), after incubating in the dark at room temperature for 20min, measure the absorbance at 535nm (OD 535 ). The sterile medium was treated the same as above and set to zero as a control. With concentrations of 0, 0.01, 0.05, 0.25, 0.5mg·mL -1 The IAA standard solution was used to make a standard curve in the same way. The unit ...

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Abstract

The invention discloses a plant growth-promoting rhizobacterium SXH-3. The plant growth-promoting rhizobacterium is classified and named as Klebsiella oxytoca SXH-3 and is preserved in China General Microbiological Culture Collection Center (CGMCC) with an accession number of CGMCC No. 6298. The Klebsiella oxytoca SXH-3 can synthesize heteroauxin and Siderophore and has an effect on dissolving phosphorus, so the Klebsiella oxytoca SXH-3 can provide plants with heteroauxin and Siderophore and promote absorption of phosphorus by the plants, thereby promoting growth of the plants. The Klebsiella oxytoca SXH-3 has a substantial promotion effect on plant height, fresh weight and dry weight of leek.

Description

technical field [0001] The invention belongs to the technical field of plant growth-promoting bacteria, and relates to a rhizosphere growth-promoting bacteria SXH-3 and its application. Background technique [0002] Leek (Allium tuberosum Rottl.ex Spr.) is a perennial perennial plant of the genus Allium tuberosum in the family Liliaceae. It is native to China and is cultivated all over the country. Leek is rich in vitamins and various minerals, and has a special aroma because it contains a variety of sulfides, which can increase appetite and has certain medicinal value. Its seeds and leaves can be used as medicine. It has the effects of invigorating the stomach, refreshing, antiperspirant and astringent, tonifying the kidney and yang, and strengthening essence. In traditional Chinese medicine, leek has a very famous name called "Yangyang Grass", and some people call it "Intestinal Cleansing Grass". Not only that, but there are many names for leek. Leeks are also called gr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/88C12P17/10C12P1/04A01N63/00A01P21/00C05F11/08C05G3/00C12R1/22
Inventor 郭长虹史煦涵刘佳明蔡洪生张程
Owner HARBIN NORMAL UNIVERSITY
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