Unlock instant, AI-driven research and patent intelligence for your innovation.

HIV (Human immunodeficiency virus) infection disease progression molecule marker miR-99a

A technology of mir-99a and molecular markers, applied in the field of miR-99a, a molecular marker of HIV infection disease progression, can solve the problems of lack of research and achieve the effect of simple operation, high sensitivity, and easy mass screening

Active Publication Date: 2013-09-18
THE FIRST HOSPITAL OF CHINA MEDICIAL UNIV +1
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some studies have been carried out in this field, the miRNA expression changes in HIV-infected patients with early disease progression have not been studied so far. In clinical and research, it is necessary to find miRNA markers that can effectively predict disease prognosis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • HIV (Human immunodeficiency virus) infection disease progression molecule marker miR-99a
  • HIV (Human immunodeficiency virus) infection disease progression molecule marker miR-99a
  • HIV (Human immunodeficiency virus) infection disease progression molecule marker miR-99a

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0018] 1. Extraction of total RNA from peripheral blood mononuclear cells

[0019] 5ml of blood was drawn from 11 patients with rapid progress of HIV infection after 120 days of infection, and 5ml of blood from each of 6 typical progressors of HIV infection and 4 normal controls were taken as controls. Suspend the cells and place them in a 1.5ml RNase / DNase-free centrifuge tube.

[0020] Total RNA was extracted from peripheral blood mononuclear cells using an RNA extraction kit (Beijing Kuangbo Biotechnology Co., Ltd.), and 1 μl (20 nM) of the sequence 5'-CAACCUCCUAGAAAGA-3' (SEQ ID NO: 2) External Control-1 (synthesized by Shanghai Sangon Bioengineering Technology Co., Ltd.) to monitor the quality of RNA extraction from peripheral blood mononuclear cells. The concentration of extracted total RNA was measured using Thermo NanoDrop2000c.

[0021] 2. Three-step method for quantitative detection of miRNA in peripheral blood mononuclear cells

[0022] (1) Add polyA tail:

[00...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an HIV (human immunodeficiency virus) infection disease progression molecule marker miR-99a and application of the HIV infection disease progression molecule marker miR-99a to preparing an HIV infection disease progression diagnosis reagent. The content of miR-99a of an HIV infection rapid progression patient in single nuclei in peripheral blood is lower than that of a typical progression patient in the single nuclei of the peripheral blood, so that the disease progression degree after one year can be predicted after infection. The invention also provides a diagnosis kit for diagnosing HIV infection disease progression. Used for diagnosing the disease progression, the HIV infection disease progression molecule marker miR-99a has the characteristics of simplicity in operation, safety, no injury, high specifically, high sensitivity and easiness for large-scale screening.

Description

technical field [0001] The present invention relates to the field of prognostic diagnosis of HIV infection, and specifically relates to a molecular marker of HIV infection disease progression miR-99a, which can be applied to early prognosis assessment of HIV infection, so as to give patients timely and effective follow-up, treatment and other intervention measures , to prolong the survival time and improve the quality of life. Background technique [0002] microRNA (miRNA) is a research hotspot in recent years. It is a single-stranded small molecule RNA widely present in eukaryotes. It has no coding function, but it can bind to the flanking region of the gene sequence to repress or inhibit the translation of target mRNA. , has a high degree of conservation, timing and tissue specificity, and currently plays an important role in the clinical diagnosis, treatment and prognosis evaluation of tumors, blood diseases, viral infections and other diseases. [0003] AIDS is a major ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12Q1/68
Inventor 尚红李启靖张子宁徐俊杰付雅静
Owner THE FIRST HOSPITAL OF CHINA MEDICIAL UNIV