Cordyceps militaris fruit body cultivation technology for stabilizing cordycepin content

A technology of cordycepin and content, which is applied in the field of cultivation technology of fruiting body of Cordyceps militaris, can solve the problems of instability, lack of universal significance, unable to be put into large-scale production, etc., and achieve the effect of stable content

Active Publication Date: 2013-01-16
SHANXI WANHAIAO BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] The content of cordycepin in the product produced by the above-mentioned conventional process is 0.01-0.06%, and there are also cases with higher content, but it is unstable and has no universal significance, so it cannot be put into large-scale production

Method used

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  • Cordyceps militaris fruit body cultivation technology for stabilizing cordycepin content

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1～10

[0048] Examples 1-10 use the preservation number CGMCC No.5577 strain

[0049] In order to prove the feasibility of the technology of the present invention, during 2009 to 2012, with the preservation number CGMCC No.5577 strain, the technology of the present invention was used to produce 10 batches of products in total, and the method of each batch is as follows:

[0050]1.1 Main ingredient formula: 135g each of corn dregs, sorghum, oats, and millet, totaling 540g.

[0051] 1.2 Prepare pine needle nutrient solution: dry pine needles 648g, unpolluted water 6480ml, boil for 1.5 hours, filter, add unpolluted water to make up the filtrate to 6480ml, add 10.8g sucrose, VB1 108mg, silkworm chrysalis powder 108g, mix well.

[0052] 1.3 Take 648ml of pine needle nutrient solution, mix and impregnate with the main material for 24 hours, until the liquid is completely absorbed by the main material.

[0053] 1.4 Divide into 18 bottles (each bottle is equivalent to 30g of main dry materi...

Embodiment 11～13

[0061] The strains used in Examples 11-13 are derived from Dongfang No. 9 strains of Oriental Cordyceps Science and Technology Park, Xiajin County, Shandong Province

[0062] 2.1 Main ingredient formula: 150g each of rice, wheat, soybean flour, oats, corn dregs, and sorghum rice, totaling 900g.

[0063] 2.2 Preparation of pine needle nutrient solution: dry pine needles 1080g, unpolluted water 10800ml, boil for 1.5 hours, filter, take the filtrate, add unpolluted water to make up the filtrate to 10800ml, add whole eggs 180g, VB1 180mg, sucrose 18g, mix well.

[0064] 2.3 Take 1080ml of pine needle nutrient solution, mix it with the main ingredients and soak for 24 hours, and wait for the main ingredients to absorb all the liquid.

[0065] 2.4 Divide into 30 bottles (each bottle is equivalent to 30g of main dry material), seal, sterilize and inoculate.

[0066] 2.5 Cultivation: divided into three groups A, B, C, 10 bottles in each group.

[0067] ① Protect from light for 43 da...

Embodiment 14～16

[0069] The strain Cordyceps militaris JK5 used in Examples 14-16 was purchased from Jiangnan Biotechnology Co., Ltd., Danyang City, Jiangsu Province

[0070] 3.1 Main ingredient formula: 180g each of wheat, corn dregs, and soybean meal, totaling 540g,

[0071] 3.2 Prepare pine needle nutrient solution: dry pine needles 648g, unpolluted water 6480ml, boil for 1.5 hours, filter, take the filtrate, add unpolluted water to make up the filtrate to 6480ml, add whole egg 108g, VB1 108mg, sucrose 10.8g, mix well.

[0072] 3.3 Take 648ml of pine needle nutrient solution, mix it with the main ingredients and soak for 24 hours, and wait for the main ingredients to absorb all the liquid.

[0073] 3.4 Divide into 18 bottles (each bottle is equivalent to 30g of dry material), seal, sterilize and inoculate.

[0074] 3.5 Cultivation: divided into three groups A, B and C, with 6 bottles in each group.

[0075] ①Protect bacteria from light for 45 days and keep at 15-18°C. ②Grow grass at vari...

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Abstract

The invention provides a cordyceps militaris fruit body cultivation technology for stabilizing cordycepin content. The major ingredients of a culture medium consist of at least three of corn grit, sorghum, oat, millet, rice, wheat and soybean meal, and the content of each component is (1 / N)+ / -5% by mass, wherein N is the component number; the major ingredients of the culture medium are soaked with a pine needle nutrient solution; and the use amount of the pine needle nutrient solution is 1.2-1.4 times the weight of the major ingredients of the culture medium based on the condition that the liquid is completely absorbed by the major ingredients. A preparation method of the pine needle nutrient solution comprises the following steps of: fetching the dry Chinese pine needle growing in an environment without industrial and pesticide pollution for over 6 years, wherein the use amount of the pin needle is 1.0-1.4 times the weight of the major ingredients of the culture medium; adding 9-11 times of water and boiling for 1.5-2 hours; fetching the filtrate, and adding water to obtain initial liquid amount; and adding sucrose, VB1 and silkworm chrysalis meal, or mashing a whole egg and adding to replace the silkworm chrysalis meal, wherein the addition amount of the sucrose, VB1 and silkworm chrysalis meal or whole egg is 1.8-2.2%, 0.015-0.025% and 18-22% of the weight of the major ingredients of the culture medium respectively; and the total culture period is 62-75 days.

Description

technical field [0001] The invention belongs to the technical field of fungal sporocarp production, and in particular relates to a cultivation technique of Cordyceps militaris sporocarp. The content of cordycepin in the fruiting body of Cordyceps militaris cultivated by this process is stable and greater than 0.1%. Background technique [0002] Cordyceps militaris (Cordyceps militaris), also known as Cordyceps militaris, belongs to the genus Cordyceps of the family Ascomycetes Hypocreales. my country began to study artificial Cordyceps militaris cultivation technology from the 1980s, and has formed a conventional cultivation technology and entered the stage of industrialization. Cordyceps militaris is emerging as a new type of industry, but ordinary cultivators pay more attention to the cultivation yield and ignore the importance of product functions. 10 h 13 o 3 N 5 ) There is little research on the stable increase of content. In recent years, domestic and foreign med...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 周翔胡素花杨毅周文贵刘巧云
Owner SHANXI WANHAIAO BIOLOGICAL TECH CO LTD
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