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Method of induction and tissue culture for adventitious roots of Euphorbia fischeriana

A tissue culture, wolf venom technology, applied in the field of cell engineering, can solve problems such as no literature report, no wolf venom cell, tissue culture and production method, and achieve the effects of simple method, high content and high efficiency.

Inactive Publication Date: 2013-01-16
INST OF FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Through the retrieval of existing documents, it is found that except Dai Chuanchao and others have carried out the research on the suspension cell culture of the medicinal plant Euphorbia pekinensis (see: Dai Chuanchao, Yu Boyang, Xue Fei, Jiang Jihong, the medicinal plant Euphorbia pekinensis suspension cell culture). Cell culture, Journal of Nanjing Forestry University (Natural Science Edition), 2005, 29 (2): 57-60), there is no literature report on Euphorbia fischeriana cells, tissue culture and production methods, and there is no reference to Literature Report on Adventitious Root Induction and Adventitious Root Culture System Establishment of Euphorbia fischeriana

Method used

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  • Method of induction and tissue culture for adventitious roots of Euphorbia fischeriana
  • Method of induction and tissue culture for adventitious roots of Euphorbia fischeriana
  • Method of induction and tissue culture for adventitious roots of Euphorbia fischeriana

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Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1: the germination of wolfbane seed

[0027] Get the seeds of the wild chamaejasmea plant, through 0.2% HgCl 2 Disinfect for 12 minutes, rinse with sterile water for 3 times, place on MS solid medium without any plant growth regulator, and cultivate for 25 days at 26°C and 3000 lux light conditions, and then germinate into seedlings .

[0028] Implementation effect: the germination rate of chamaejasma seeds reaches 5.0%, and the germinated sterile seedlings have complete roots, stems and leaves, and grow normally.

Embodiment 2

[0029] Embodiment 2: Induction of chamaejasma callus

[0030] The 3 centimeter-long aseptic seedlings of chamaejasma chamaejasme that embodiment 1 obtains are placed in containing 1mg / L 2,4-dichlorophenoxyacetic acid (2,4-D), 0.5mg / L naphthaleneacetic acid, 0.1mg / L 6 -Benzylaminoadenine (6-BA) three kinds of plant growth regulators and MS solid medium of 3ml / L coconut milk, inducing 25 days under 24-26 ℃ of sterile light-proof conditions, promptly obtain the healing of chamaejasma damage tissue.

[0031] Implementation effect: the induction rate of chamaejasma chamaejasma callus reached 80.0%. The joint action of 2,4-dichlorophenoxyacetic acid (2,4-D), naphthaleneacetic acid, 6-benzylaminoadenine and coconut water promoted the formation and growth of callus.

Embodiment 3

[0032] Embodiment 3: Induction of the adventitious root of chamaejasma

[0033] Place the chamaejasma chamaejasma callus that embodiment 2 obtains on the MS solid medium containing this plant growth regulator of 10mg / L indole butyric acid and 2ml / L coconut milk, in 24-26 ℃ of sterile light-proof conditions After 25 days of induced culture, the adventitious roots of chamaejasma chamaejasma were obtained.

[0034] Implementation effect: the induction rate of adventitious roots reaches 90.0%, the adventitious roots are thin and short, and thin and short adventitious roots are produced laterally, and the adventitious roots undergo slight callus.

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Abstract

The invention relates to a method of induction and tissue culture for adventitious roots of Euphorbia fischeriana, belonging to the technical field of cell engineering. The method disclosed herein comprises the following steps: (1) seed germination: sterilizing Euphorbia fischeriana seeds with HgCl2, then putting the sterilized seeds on an MS solid medium, and germinating under illumination; (2) induction of callus: putting the young seedlings obtained by the step (1) on an MS solid medium containing 2,4-dichlorphenoxyacetic acid, naphthylacetic acid, 6-benzylamino adenine and coconut juice, and inducing in sterile and dark conditions to form callus; (3) induction of adventitious roots: putting the callus obtained by the step (2) on an MS solid medium containing indolebutyric acid and coconut juice, and inducing in sterile and dark conditions to form adventitious roots; and (4) culture of adventitious roots: putting the adventitious roots obtained by the step (3) on an MS solid medium containing indolebutyric acid to culture. The method has the advantages of simpleness and has high active substance content.

Description

technical field [0001] The invention relates to a method in the technical field of cell engineering, in particular to a method for inducing and tissue-culturing the adventitious root of chamaejasbae. Background technique [0002] Wolfbane is one of the unique and valuable Chinese herbal medicines in my country, which contains various biologically active substances such as insecticidal, bactericidal and anticancer. Diterpenoid Prostratin ( figure 1 ) was first isolated from the New Zealand plant Pimelea prostrata by Cashmore et al. In recent years, people have also isolated the diterpenoids from the Mamala tree Homalanthus nutans in Samoa, the Pacific island country, and found that Prostratin can activate the latent HIV virus, showing good anti-HIV activity, and it is expected to be developed into a new drug, but people The research found that the resources of Mamala tree are very few, and the content of Prostratin in it is very low. Chinese scholars have found that the ro...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 邱德有刘洪伟杨艳芳张楠
Owner INST OF FORESTRY CHINESE ACAD OF FORESTRY
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