The invention discloses disinfectant for a clinical laboratory and a preparation method of the disinfectant, and relates to the field of traditional Chinese medicine. The active ingredients of the disinfectant consist of the following ingredients including angelica, eucalyptus globulus, lalang grass rhizome, mangnolia officinalis, resina toxicodendri, hemerocallis fulva, solanum mauritianum, fructus chebulae, Chinese torreya, Euphorbia fischeriana steud, juniper berry, golden larch bark, fructus forsythiae, short-tube lycoris, purslane and dark plum. The disinfectant for the clinical laboratory adopts natural ingredients, is a pure traditional Chinese medicine preparation, is prepared by adopting the modern advanced medicine pharmacy technology, and has the advantages of high disinfection capability, high acting speed, high stability, no toxicity, no corrosion, small irritation, easy water solubility, low price, easy acquisition and the like, in addition, the disinfectant is safe and harmless on people and animals, the environment pollution degree is low, and the disinfectant belongs to ideal broad-spectrum disinfectant.