The invention relates to a plaster and a preparation method thereof, in particular to a plaster capable of treating lumbar intervertebral protrusion, scapulohumeral periarthritis, cervical spondylosis, ankylosing spondylitis and hyperostosis and a preparation method of the plaster. The plaster is prepared from long-noded pit viper, pangolin, centipede, scorpion, monkshood, kusnezoff monkshood root, clematis root, divaricate saposhnikovia root, figwort, eucalyptus globulus labill, frankincense, myrrh, dragon's blood, keel, cuttlebone, red halloysite, calomel, borneol, musk, camphor, ferula and the like through soaking, decoction, filtration, concentration, addition of minium and removal of fire toxin. The plaster is not required to be orally taken, does not stimulate five organs and is stuck to the sick part and the efficacy of the plaster can be naturally absorbed through skin pores; and the plaster can be used for treating the lumbar intervertebral protrusion, scapulohumeral periarthritis, cervical spondylosis, ankylosing spondylitis and hyperostosis and directly reach the focus, is quick in effect taking, and does not relapse.