The invention relates to the technical field of
plant tissue culture, and particularly discloses a
tissue culture and rapid propagation method of
eucalyptus luminocarpa, which comprises the following steps: (1) selecting an explant, removing leaves, sterilizing the surface, and
cutting small stem sections; (2) inoculating the small stem segments into an induction culture medium, and performing dark culture to obtain calluses; (3) transferring the calluses into a differentiation
solid culture medium, and performing differentiation culture to obtain differentiated buds; (4) inoculating the differentiated buds into a proliferation culture medium, and carrying out suspension proliferation culture to obtain cluster buds; (5) selecting cluster
bud single plants, inoculating the cluster
bud single plants into a rooting culture medium, and inducing rooting culture to obtain
tissue culture seedlings; and (6) selecting tissue culture seedlings, transferring the tissue culture seedlings to a
greenhouse, opening a cover, hardening the seedlings, and
transplanting the seedlings into a culture medium to obtain
eucalyptus citrina plants. According to the tissue culture and rapid propagation method of the
eucalyptus globulus, the explant
pollution rate is low, the
callus induction rate and the differentiation rate are high, the
browning phenomenon in the tissue culture process can be inhibited, the tissue activity is protected, the robust development of a
root system is promoted, and the rooting effect is improved.