2 results about "Eucalyptus globulus" patented technology

This invention discloses a cream for treating psoriasis vulgaris and its preparation method: belonging to the technical field of psoriasis drug technology, this cream is made from the following raw materials in parts by weight: 1-5 parts Tripterygium wilfordii, 1-5 parts Euphorbia fischeriana, 10 parts Catechu, 8 parts Eucalyptus globulus, 10 parts Dictamnus dasycarpus, 10 parts Spatholobus suberectus, 10 parts Sophora flavescens, 10 parts Rehmannia glutinosa, 10 parts Angelica sinensis, 100-140 parts water, 40-50 parts emulsifier, and 0.05-0.1 parts preservative. The preparation method includes: decocting Tripterygium wilfordii, Euphorbia fischeriana, Catechu, Eucalyptus globulus, Dictamnus dasycarpus, Spatholobus suberectus, Sophora flavescens, Rehmannia glutinosa, and Angelica sinensis with water, then removing the herbs to obtain a medicinal paste; mixing the medicinal paste, emulsifier, and preservative and adding them to an emulsifier for emulsification to obtain the product. This formula can treat psoriasis vulgaris. As a topical cream for psoriasis, this invention has antifungal, antiviral, heat-clearing and blood-cooling, and antioxidant activities. It can inhibit epidermal cell proliferation, slow down the epidermal metabolic cycle, promote keratinization, inhibit sebum secretion, inhibit the growth environment of bacteria and fungi, and relieve itching caused by inflammation.

本发明属于植物基因工程技术领域，具体是一种蓝桉抗旱相关转录因子基因EgDREB2A及其编码蛋白与应用。本发明从蓝桉（Eucalyptus globulus）中克隆获得EgDREB2A基因，其核苷酸序列如SEQ ID NO:1所示，编码蛋白的氨基酸序列如SEQ ID NO:2所示，包含AP2 DNA结合结构域（第58‑117位氨基酸）。该基因受干旱胁迫强烈诱导表达，24h达峰值（18.6倍）。本发明构建了含EgDREB2A的重组表达载体，通过农杆菌介导法转化蓝桉，获得转基因植株。转基因植株在干旱胁迫下表现出显著增强的抗旱性：叶片相对含水量提高60%，脯氨酸含量提高3.4倍，电解质渗漏率降低50%，且下游抗旱基因Rd29A、P5CS、LEA显著上调表达。亚细胞定位显示EgDREB2A蛋白定位于细胞核，符合转录因子特征。本发明为培育抗旱蓝桉新品种提供了重要基因资源和有效方法。