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Method for promoting reproduction of melaleuca alternifolia cluster buds and avoiding subculture browning

A technology of Melaleuca alternifolia, multiplication and cultivation, which is applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., and can solve the problems that rare earth elements have not been reported.

Inactive Publication Date: 2013-01-16
YULIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the application of rare earth elements in promoting the bud formation of Melaleuca alternifolia and preventing browning in subculture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1. Prepare the following four media:

[0017] Control medium CK: add 7g / L agar, 1.0mg / L6-BA, 0.01mg / L 2,4-D to the modified MS medium;

[0018] Medium A: Add 7g / L agar, 1.0mg / L 6-BA, and 3mg / L neodymium nitrate [Nd(NO 3 ) 3 ];

[0019] Medium B: Add 7g / L agar, 0.01mg / L 2,4-D to the modified MS medium, and then add 3mg / L neodymium nitrate [Nd(NO 3 ) 3 ];

[0020] Medium C: add 7g / L agar, 1.0mg / L 6-BA, 0.01mg / L2, 4-D to the modified MS medium; then add 1mg / L neodymium nitrate [Nd(NO 3 ) 3 ].

[0021] 2. Insert the adventitious buds of Melaleuca alternifolia into the above-mentioned four kinds of medium, under the condition of temperature 26°C and light of 2000 lux, light for 12 hours a day, and cultivate for 25 days, the results are as follows:

[0022] When cultured in the control medium CK, the clustered shoots multiplied by 1.8 times, and the subculture browning rate was 25% on the 15th day of culture, and 33% on the 25th day of culture;

[0023] Cultivated in...

Embodiment 2

[0027] 1. Prepare the following four media:

[0028] Control medium CK: add 7g / L agar, 1.0mg / L6-BA, 0.01mg / L 2,4-D to the modified MS medium;

[0029] Medium A: Add 7g / L agar, 1.0mg / L 6-BA, and 5mg / L lanthanum nitrate [La(NO 3 ) 3 ];

[0030] Medium B: Add 7g / L agar, 0.01mg / L 2,4-D to the modified MS medium, then add 5mg / L lanthanum nitrate [La(NO 3 ) 3 ];

[0031] Medium C: add 7g / L agar, 1.0mg / L 6-BA, 0.01mg / L2, 4-D to the modified MS medium; add 2mg / L lanthanum nitrate [La(NO 3 ) 3 ].

[0032] 2. Insert the adventitious buds of Melaleuca alternifolia into the above-mentioned four culture media, under the conditions of temperature 25°C and light of 2500 lux, light for 12 hours a day, and cultivate for 27 days, the results are as follows:

[0033] When cultured in the control medium CK, the clustered shoots multiplied by 2.1 times, and the subculture browning rate was 27% on the 15th day of culture, and 34% on the 25th day of culture;

[0034] Cultivated in medium A,...

Embodiment 3

[0038] 1. Prepare the following four media:

[0039] Control medium CK: add 7g / L agar, 1.0mg / L6-BA, 0.01mg / L 2,4-D to the modified MS medium;

[0040] Medium A: Add 7g / L agar, 1.0mg / L 6-BA, and 1mg / L praseodymium (III, IV) [Pr 6 o 11 (III, IV)] in nitric acid solution;

[0041] Medium B: Add 7g / L agar, 0.01mg / L 2,4-D to the modified MS medium, and then add 1mg / L praseodymium (III, IV) [Pr 6 o 11 (III, IV)] in nitric acid solution;

[0042] Medium C: add 7g / L agar, 1.0mg / L 6-BA, 0.01mg / L2, 4-D to the modified MS medium; then add 0.5mg / L praseodymium (III, IV) [Pr 6 o 11 (III, IV)] in nitric acid solution.

[0043] 2. Insert the adventitious buds of Melaleuca alternifolia into the above-mentioned four culture media, under the conditions of temperature 25°C and light of 3000 lux, light for 12 hours a day, and cultivate for 20 days, the results are as follows:

[0044]When cultured in the control medium CK, the clustered shoots multiplied by 2.9 times, and the subculture b...

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PUM

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Abstract

The invention relates to a method for promoting reproduction of melaleuca alternifolia cluster buds and avoiding subculture browning. According to the method, adventitious buds of melaleuca alternifolia are inoculated on an improved MS culture medium containing rare-earth elements, mitogen and auxin to perform enrichment culture, reproduction of the melaleuca alternifolia cluster buds can be effectively promoted, and subculture browning can be effectively avoided. A single or mixed rare-earth element compound comprises nitrates and oxides of lanthanum, cerium, neodymium, praseodymium and samarium, and the rare-earth elements can reduce or replace the auxin or cytokinin in the culture medium.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a method for promoting the multiplication of Melaleuca alternifolia cluster buds and preventing subculture browning. Background technique [0002] Melaleuca leucadendron is a small evergreen tree of the genus Melaleuca in the family Myrtaceae. The tea tree oil extracted from its fresh branches, leaves and trunk is a powerful antibacterial and immune The activator is recognized as an excellent natural health care medical material, and is widely used in pharmaceuticals, daily chemicals, food, spices and other industries. Melaleuca alternifolia has the characteristics of fast growth, straight trunk, strong germination, etc. After planting, it can be harvested many times, and has extremely high economic benefits. At present, it has been promoted in many provinces (regions) such as Guangxi, Guangdong, and Fujian. . At present, Melaleuca alternifolia is mainly pr...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 莫昭展梁海清朱宇林黄肈宇
Owner YULIN NORMAL UNIVERSITY
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