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Method for extracting bone calcium through defatting fermentation and bone gla protein

An extraction method and bone calcium technology, applied in the field of food processing, can solve the problems of difficult to remove bone fat and high fat content in products, and achieve the effect of increasing economic burden

Active Publication Date: 2013-01-16
TIANJIN TIANSHI BIOLOGICAL DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, in the bone calcium prepared by the above process, the degreasing method is mainly implemented by cooking, which is difficult to remove the high content of fat in the bone, resulting in a higher fat content in the final product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Take animal bones and crush them to 40mm particles, add ethanol, extract at 80°C for 75min, discard the supernatant; soak and rinse in hot water at 90°C for 30min, centrifuge the aggregate at 8000r / min for 30min, discard the supernatant; Add water to the aggregate, the weight volume ratio of aggregate to water is 1:5, adjust the pH value to 10, add alkaline lipase A, B compound lipase mixed at 1.5:1, account for 0.4% of the aggregate ratio, 30°C, hydrolyze for 60min , centrifuged, and supernatant removed; Bifidobacterium lactis (B.lactis), Lactobacillus bulgaricus (L.bulgaricus) and Lactobacillus helveticus (L.helviticus), three kinds of lactic acid bacteria weight ratio 1:3:2; aggregate and Water weight volume ratio 1:5, add 5% sucrose of the total weight, lactic acid bacteria account for 0.04% by weight of the aggregate, 50°C, ferment for 12h, sterilize at 120°C for 30min; add hydrochloric acid to adjust the pH value to 3, add pepsin, Accounting for 0.08% of the aggre...

Embodiment 2

[0035]Take animal bones and crush them to 5mm particles, add ethanol, leaching at 60°C for 45min, discard the supernatant; soak and rinse in hot water at 80°C for 30min, centrifuge the aggregate at 4000r / min for 30min, discard the supernatant; Add water to the aggregate, the weight volume ratio of aggregate to water is 1:3, adjust the pH value to 8, add alkaline lipase A, B compound lipase mixed at 3:1, accounting for 0.1% of the aggregate ratio, 20°C, hydrolyze for 40 minutes , centrifuged, and supernatant removed; Bifidobacterium lactis (B.lactis), Lactobacillus bulgaricus (L.bulgaricus) and Lactobacillus helveticus (L.helviticus), three kinds of lactic acid bacteria weight ratio 1:3:2; aggregate and Water weight volume ratio 1:3, add 5% sucrose of the total weight, lactic acid bacteria account for 0.01% by weight of the aggregate, 35°C, ferment for 12h, sterilize at 120°C for 30min; add hydrochloric acid to adjust the pH value to 1, add pepsin, Accounting for 0.04% of the a...

Embodiment 3

[0037] Crush animal bones to 20mm particles, add ethanol, extract at 70°C for 60 minutes, discard the supernatant; soak and rinse in hot water at 85°C for 30 minutes, centrifuge the aggregate at 8000r / min for 30 minutes, discard the supernatant; Add water to the aggregate, the weight-volume ratio of aggregate to water is 1:4, adjust the pH value to 9, add alkaline lipase A, B compound lipase mixed at 2:1, accounting for 0.3% of the aggregate ratio, 25°C, hydrolyze for 50 minutes , centrifuged, and supernatant removed; Bifidobacterium lactis (B.lactis), Lactobacillus bulgaricus (L.bulgaricus) and Lactobacillus helveticus (L.helviticus), three kinds of lactic acid bacteria weight ratio 1:3:2; aggregate and The weight-to-volume ratio of water is 1:4, adding 5% sucrose to the total weight, lactic acid bacteria accounting for 0.03% by weight of the aggregate, fermenting at 40°C for 12 hours, and sterilizing at 120°C for 30 minutes; adding hydrochloric acid to adjust the pH value to ...

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Abstract

The invention discloses a method for extracting bone calcium through defatting fermentation and bone gla protein prepared by means of the method. The method comprises the following steps of three-time defatting, two-time centrifugation, fermentation and protease hydrolysis. Fat in bone meal prepared by adopting a defatting process is completely removed basically, so that the fat content in calcium obtained by means of enzymolysis is lower than 0.1%, and the intracellular free calcium content is increased. The bone gla protein is safe and healthy to consumers with cardiovascular diseases, and economical burden to the consumers is not increased.

Description

technical field [0001] The invention relates to a bone calcium extraction process and belongs to the field of food processing. Background technique [0002] With the aging of society, the prevalence of osteoporosis ranks first among metabolic bone diseases in western countries. The pain that osteoporosis brings to patients and families is self-evident. According to statistics, there are currently more than 100 million osteoporosis patients in China, and it is estimated that the number will increase to 210 million by 2050. Factors causing calcium deficiency in more and more people: Accelerated pace of life and work, high work pressure and irregular life, not paying attention to the reasonable collocation of dietary structure, those with a daily calcium intake of less than 600 mg can be considered as calcium intake Insufficient calcium in the body can easily lead to lack of calcium in the body; some middle-aged people are stressed at work, lack self-care awareness, and do no...

Claims

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Application Information

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IPC IPC(8): A23L1/29A23L1/312A23L33/00A23L13/20
Inventor 郁正刚吴益群赵健崔卜东黄永亮
Owner TIANJIN TIANSHI BIOLOGICAL DEV
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