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Bone calcium extraction method capable of reducing fat content, and osteocalcin

An extraction method and fat content technology, which are applied in the field of food processing, can solve the problems of difficult to remove bone fat and high fat content in products, and achieve the effect of increasing economic burden.

Inactive Publication Date: 2013-01-16
TIENS BIOTECH GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, in the bone calcium prepared by the above process, the degreasing method is mainly implemented by cooking, which is difficult to remove the high content of fat in the bone, resulting in a higher fat content in the final product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Take animal bones and crush them to 40mm particles, add ethanol, extract at 80°C for 75min, discard the supernatant; soak and rinse in hot water at 90°C for 30min, centrifuge the aggregate at 8000r / min for 30min, discard the supernatant; Add water to the aggregate, the weight volume ratio of aggregate to water is 1:5, adjust the pH value to 10, add alkaline lipase A, B compound lipase mixed at 1.5:1, accounting for 0.4% of the aggregate weight ratio, 30°C, hydrolyze 60min, centrifuge, discard the supernatant; add water, the weight volume ratio of aggregate to water is 1:5, 100°C, 10min, cool to 55°C, add trypsin, accounting for 0.08% of aggregate weight, stir for 2h; Protease, accounting for 0.1% by weight of the aggregate, stirred for 2 hours, separated from the liquid, inactivated at high temperature, and dried to obtain the product. The fat content of the bone meal was measured by the detection method specified in "GB / T5009.6 Determination of Fat in Food", and the fa...

Embodiment 2

[0022] Take animal bones and crush them to 5mm particles, add ethanol, leaching at 60°C for 45min, discard the supernatant; soak and rinse in hot water at 80°C for 30min, centrifuge the aggregate at 4000r / min for 30min, discard the supernatant; Add water to the aggregate, the weight volume ratio of aggregate to water is 1:3, adjust the pH value to 8, add alkaline lipase A, B mixed at 3:1 compound lipase, accounting for 0.1% of the aggregate weight ratio, 20°C, hydrolyze 40min, centrifuge, discard the supernatant; add water, the weight volume ratio of aggregate to water is 1:3, 90°C, 10min, cool to 45°C, add trypsin, accounting for 0.04% of aggregate weight, stir for 1h; add medium Protease, accounting for 0.05% by weight of the aggregate, stirred for 1 hour, separated from the liquid, inactivated at high temperature, and dried to obtain the product. The fat content of the bone meal was measured by the detection method specified in "GB / T5009.6 Determination of Fat in Food", and...

Embodiment 3

[0024] Crush animal bones to 20mm particles, add ethanol, extract at 70°C for 60 minutes, discard the supernatant; soak and rinse in hot water at 85°C for 30 minutes, centrifuge the aggregate at 8000r / min for 30 minutes, discard the supernatant; Add water to the aggregate, the weight volume ratio of aggregate to water is 1:4, adjust the pH value to 9, add alkaline lipase A, B compound lipase mixed at 2:1, accounting for 0.3% of the aggregate weight ratio, 25°C, hydrolyze 50min, centrifuge, discard the supernatant; add water, the weight volume ratio of aggregate to water is 1:4, 95°C, 10min, cool to 50°C, add trypsin, accounting for 0.06% of aggregate weight, stir for 1.5h; add Neutral protease, accounting for 0.08% by weight of the aggregate, stirred for 1.5 hours, separated from the liquid, inactivated at high temperature, and dried to obtain the product. The fat content of the bone meal was measured by the detection method specified in "GB / T5009.6 Determination of Fat in Foo...

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Abstract

The invention discloses a bone calcium extraction method capable of reducing fat content, and osteocalcin prepared by the method. The method comprises the following steps of three times of derosination, twice centrifugation and a two-stage protease hydrolysis. Fat in bone powder prepared by the derosination process of the invention is completely removed basically, allowing the fat content in the calcium preparation obtained by enzymatic hydrolysis to be lower than 0.1%. The product is safer and healthier for consumers with cardiovascular diseases, but does not increase economic burden.

Description

technical field [0001] The invention relates to a bone calcium extraction process and belongs to the field of food processing. Background technique [0002] With the aging of society, the prevalence of osteoporosis ranks first among metabolic bone diseases in western countries. The pain that osteoporosis brings to patients and families is self-evident. According to statistics, there are currently more than 100 million osteoporosis patients in China, and it is estimated that the number will increase to 210 million by 2050. Factors causing calcium deficiency in more and more people: Accelerated pace of life and work, high work pressure and irregular life, not paying attention to the reasonable collocation of dietary structure, those with a daily calcium intake of less than 600 mg can be considered as calcium intake Insufficient calcium in the body can easily lead to lack of calcium in the body; some middle-aged people are stressed at work, lack self-care awareness, and do no...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/29A23L1/312A23L33/00A23L13/20
Inventor 赵健黄永亮郁正刚崔卜东吴益群
Owner TIENS BIOTECH GROUP
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