Colloidal gold sensitization chromatography test strip for quickly detecting copper ions

A technology for detecting test strips and copper ions, applied in the field of immunology, to achieve high labeling rate, strong specificity, and high sensitivity

Inactive Publication Date: 2013-01-16
中华人民共和国淮安出入境检验检疫局 +1
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] At present, the application of enzyme-linked immunoassay technology and test strip technolog

Method used

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  • Colloidal gold sensitization chromatography test strip for quickly detecting copper ions
  • Colloidal gold sensitization chromatography test strip for quickly detecting copper ions

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Experimental program
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Embodiment Construction

[0020] To make a copper ion detection test strip, it is first necessary to prepare a carrier protein coupled to copper ions, which is used to prepare the corresponding detection line (T line) and immunogen to prepare antibodies.

[0021] 1. Coupling of copper to carrier protein

[0022] Immunization Antigen Copper-1-(4-isothiocyanobenzyl)-vinyldiamine- N, N, N', N' -tetraacetic acid - Preparation of keyhole limpet hemocyanin (Cu-EDTA-KLH).

[0023] First add 2 mg of 1-(4-isothiocyanatobenzyl)-vinyldiamine- N, N, N', N' -Tetraacetic acid (ITCBE) and 4.5mg of KLH protein were reacted in 2-[4-(2-hydroxyethyl)-1-piperazine]ethanesulfonic acid (HEPES) buffer at pH 9.4 for 24h, and then incubated at 4°C , 8000r / min, ultrafiltration for 10min to remove uncoupled ITCBE, then wash the retentate with pH 7.4 HEPES buffer, then add 1 mg / mL Cu 2+ After reacting with 1000 μL of standard solution for 1 hour, use an ultrafiltration centrifuge tube to remove unchelated Cu 2+ , and then di...

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Abstract

The invention relates to a colloidal gold sensitization chromatography test strip for quickly detecting copper ions, which belongs to the technical field of immunology, and comprises a lining board, a sample cushion, a gold-marking combined cushion, a sensitization cushion, a coating film and a water absorption cushion, wherein the sample cushion, the gold-marking combined cushion, the sensitization cushion, the coating film and the water absorption cushion are sequentially jointed on the lining board; the gold-marking combined cushion is made of glass fiber cotton and used for absorbing small gold mark copper ion antibodies; the sensitization cushion is made of glass fiber cotton and used for absorbing large gold mark goat anti-mouse IgG (immunoglobulin G); and the coating film is provided with a linear invisible detection line T and a linear invisible control line C which are arrayed in parallel, wherein the linear invisible detection line T is printed by the solution formed by the coupling of the copper ions and carrier protein, and the linear invisible control line C is printed by the goat anti-mouse IgG solution. The colloidal gold sensitization chromatography test strip for quickly detecting the copper ions, provided by the invention, has the advantages of strong specificity and high sensitivity, can detect the residue of the copper ions more quickly, sensitively, simply and conveniently, is lifelike, intuitive, accurate, simple and clear in result determination, is not easy to appear human misjudgment such as false positive, false negative and the like, saves the expense, has wide application range, facilitates the popularization, and has wide market prospect and remarkable economic and social benefits.

Description

technical field [0001] The invention relates to a colloidal gold-sensitized chromatography test strip for rapidly detecting copper ions, a preparation method and application thereof, and belongs to the technical field of immunology. Background technique [0002] Copper is one of the heavy metals used in a relatively large amount in the world. Modern industry, the use of pesticides and fertilizers, and the copper associated with high-copper animal additives are increasingly polluting the environment. Copper is widely distributed in nature and plays an important role in animals, plants and human life. High concentrations of copper have negative effects on aquatic organisms and ecosystems, inhibiting the self-purification ability of rivers and disrupting the biological balance system of water. When the copper content in water reaches 0.01mg / L, it will have a significant inhibitory effect on the self-purification of the water body; if it exceeds 3.0mg / L, it will produce peculia...

Claims

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Application Information

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IPC IPC(8): G01N33/577
Inventor 冯民邢常瑞匡华王小晋王利兵胥传来刘丽强朱臻怡何健唐政戴建平
Owner 中华人民共和国淮安出入境检验检疫局
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