Serum/plasma miRNA (micro ribonucleic acid) marker for early detecting mandibular prognathism and application thereof
An early detection and marker technology, applied in the direction of DNA/RNA fragments, recombinant DNA technology, etc., to achieve the effect of convenient collection, accurate quantification, and easy implementation
Inactive Publication Date: 2014-04-16
NANJING MEDICAL UNIV
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[0007] Recent studies have shown that serum / plasma miRNA markers play an important role in the regulation of skeletal and craniomaxillofacial development, and can be used for the early diagnosis and treatment guidance of many diseases, but there is no information about serum / plasma miRNA for the detection of premandibular Contingency reports
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Embodiment 1
[0037] Sample collection and arrangement:
[0038] From July 2011 to August 2012, the inventor collected 128 samples (serum samples of 60 cases of mandibular protrusion and 68 cases of serum samples of normal controls) from the Department of Orthodontics, Stomatological Hospital Affiliated to Nanjing Medical University. Research.
[0039] Selection criteria for the study sample:
[0040] (1) The clinical profile of the patient is concave;
[0041] (2) The intraoral and model examinations are anterior crossbite;
[0042] (3) The X-ray showed that the maxilla was normal and the mandible was protruding;
[0043] (4) Normal controls in the growth period matched with the age of the cases.
[0044] microarray chip sequencing:
[0045] Serum samples from 18 patients with mandibular protrusion in growth stage and 18 normal controls were randomly selected from the samples collected by microarray biochip. Chip testing was performed by Kangchen Biotechnology Co., Ltd. (Shanghai, Ch...
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The invention relates to a serum / plasma miRNA (micro ribonucleic acid) marker for early detecting mandibular prognathism and application thereof. The marker is one of let-7i-3p or miR-595 or a combination thereof. A detection reagent or detection tool developed on the basis of the serum / plasma miRNA marker provides data support to the research on a mandibular prognathism occurrence and development mechanism. Through the development and application of a serum / plasma miRNA marker diagnosis kit, the early detection of mandibular prognathism is convenient and easy to implement, so that strong support can be provided to orthodontic clinicians for finding osteal mandibular prognathism as early as possible and adopting an effective and appropriate treatment scheme, thereby effectively reducing the treatment time, avoiding unnecessary repeated treatment, being capable of performing treatment dynamic evaluation and prognosis monitoring on cases, and then being beneficial to reducing the relapse of mandibular prognathism.
Description
technical field [0001] The invention belongs to the field of biotechnology, and relates to a serum / plasma miRNA marker for early detection of mandibular protrusion patients and application thereof. Background technique [0002] Anterior crossbite caused by mandibular protrusion is a common deformity in orthodontics. Anterior crossbite, commonly known as "underbite", means that the upper front teeth are located inside the lower front teeth during a centric bite, which can affect the appearance of the teeth and facial shape, reduce the efficiency of mastication, and even lead to temporomandibular joint disease and affect mental health. Due to the complex craniofacial structure and unpredictable mandibular growth direction, the treatment of skeletal inversion caused by mandibular protrusion is very difficult, and it is easy to relapse after treatment. Some patients still need orthodontics and orthodontics after 5-8 years of treatment. Combined treatment with jaw surgery. Oste...
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IPC IPC(8): C12Q1/68C12N15/11
Inventor 马俊青王林王震东谷妍张阳
Owner NANJING MEDICAL UNIV