Unlock instant, AI-driven research and patent intelligence for your innovation.

Cannula used for experimental animal bronchoalveolar lavage

A technology for experimental animals and bronchus, applied in the direction of trocars, etc., can solve the problems of large difference in recovery rate of lavage fluid and inconvenient operation, so as to increase the success rate of lavage, easy to operate, and avoid the difference in recovery rate of lavage fluid Effect

Inactive Publication Date: 2013-01-30
THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the above-mentioned deficiencies in the prior art, the present invention proposes an intubation tube for bronchoalveolar lavage of experimental animals to improve the success rate of lavage and solve the problems of large difference in recovery rate of lavage fluid and inconvenient operation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cannula used for experimental animal bronchoalveolar lavage
  • Cannula used for experimental animal bronchoalveolar lavage
  • Cannula used for experimental animal bronchoalveolar lavage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Preparation of Bronchoalveolar Lavage Cannula

[0020] The length of the metal inner tube is about 1.5cm, the inner diameter of the lumen is 1-1.5mm, and the outer diameter is 2mm. The length of the innermost sleeve of the plastic outer tube is 4cm, and the front end is an oblique opening. The plastic outer tube is tightly combined with the rear end of the metal inner tube, and is completely sealed. The diameter of the tube is 2mm. Three or four layers of sleeves 4, 5 and 6, each layer of sleeves is about 1 cm long, and marked with scales, and the diameter of the intubation tube increases by a certain length for each additional layer of sleeves. The sleeves of each layer are connected by sliding fit, which can be stretched. A sealing ring 10 is set between them for sealing, and a limit structure 7 is set between each sleeve at the two ends. The limit structure adopts a slot type . Due to the different diameters, it can be used as the intubation tube for bro...

Embodiment 2

[0021] Example 2: Bronchoalveolar Lavage in BALB / c Mice

[0022] Take BALB / c mice commonly used in experiments as an example: the diameter of the trachea of ​​18-30g BALB / c mice is about 2.5-3.5mm, and the bronchoalveolar lavage cannula prepared by the above method is used to perform bronchoalveolar lavage on the mice. After the animal died, a small opening was cut on its trachea, and the intubation tube was inserted from the opening of the trachea. Because the diameter of the mouse trachea is small, the intubation part inserted into the trachea only contains the metal inner tube and the innermost cannula, and the insertion depth is at least 1-1.5cm is appropriate (more than 2cm is easy to enter the main bronchus on one side), and the diameter of the second and third layers of cannula is just enough to support the trachea, and the trachea and intubation tube are clamped with tube clamps. Inject 1ml of pre-cooled PBS buffer solution into the lungs, gently massage the lungs with...

Embodiment 3

[0023] Embodiment 3: carry out bronchoalveolar lavage to SD rat

[0024] The diameter of the trachea of ​​a 180-220 g SD rat is about 10-15 mm, and the bronchoalveolar lavage cannula prepared by the above method is used to perform bronchoalveolar lavage on the rat. After the death of the animal, cut a small opening on its trachea, slide the second and third layers of plastic sleeves and fix them with a limiting structure, insert the intubation tube from the opening of the trachea, and insert the rat trachea into the metal inner tube, the innermost sleeve Tube, second, and third-layer plastic sleeves, the insertion depth should be 1.5-2cm (more than 3cm, it is easy to enter the main bronchi on one side), and the trachea and intubation tube are clamped with tube clamps. Inject 3ml of pre-cooled PBS into the lungs, gently massage the lungs with hands, and after repeated suction for 3 times, suck out the liquid to obtain BALF, a total of 3 times.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention claims for protecting a cannula used for experimental animal bronchoalveolar lavage. The cannula comprises a plastic outer tube and a metal inner tube. The plastic outer tube is sheathed outside the metal inner tube; the front end of the plastic outer tube is longer than the front end of the metal inner tube; the rear end of the metal inner tube is mutually connected with a hose; a connector mutually connected with a syringe is designed at the outermost end of the hose; and the rear end of the plastic outer tube and the metal inner tube are sealed and are fixedly connected. The front end of the plastic outer tube is a bevel end, and the front end of the metal inner tube is a plain end. The plastic outer tube is of a multi-layer telescopic casing pipe structure. By adopting the cannula, animal bronchoalveolar lavage fluid (BALF) can be conveniently extracted; the BALF recovery rate can be effectively improved; the operation is simple and convenient; the accuracy is high; experimental errors can be reduced; and the BALF can be reused. The cannula not only can be used for extracting large animals but also can be used for extracting small animals.

Description

technical field [0001] The invention relates to an apparatus for animal experiments, in particular to a device for obtaining animal bronchoalveolar lavage fluid. Background technique [0002] Bronchoalveolar lavage fluid (BALF) examination is of great significance for the diagnosis, curative effect evaluation and prognosis evaluation of respiratory and lung diseases. For example, observing and detecting the cellular components and tumor markers of BALF is helpful for the diagnosis and efficacy evaluation of lung cancer. Smear observation and culture of microorganisms in BALF can be used for etiological diagnosis of pulmonary infectious diseases. Analysis of the total number and differential counts of BALF cells is helpful to distinguish asthma, eosinophilic bronchitis and chronic obstructive pulmonary disease, assess the severity of airway inflammation in the above diseases, and evaluate the efficacy of drugs. Analysis of the components in BALF is helpful for the diagnosis...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61B10/02
Inventor 卓文磊王彦张亮
Owner THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV