Dynamic PCR (Polymerase Chain Reaction) and CE (capillary electrophoresis) functional integrated micro-fluidic chip of microcavity

Abstract

The invention relates to a dynamic PCR (Polymerase Chain Reaction) and CE (capillary electrophoresis) functional integrated micro-fluidic chip of a microcavity, which is a four-layer structure, wherein a third layer is provided with a degenerative microcavity, an annealing microcavity, an extending microcavity, a formamide sampling tank, a mixer, a sample tank, a buffer solution tank, a sample liquid waste tank, a liquid waste tank, a channel between the degenerative microcavity and the annealing microcavity, a channel between the annealing microcavity and the extending microcavity, a channel between the extending microcavity and the mixer, a channel between the formamide sampling tank and the mixer, a channel between the mixer and the sampling tank, and a cross channel; a first layer is equipped with a heating electrode, a temperature sensing electrode, electrode points and high voltage electrodes, wherein the heating electrode and the temperature sensing electrode are opposite to the positions of the degenerative microcavity, the annealing microcavity and the extending microcavity, the electrode points are opposite to the positions of the sampling tank, the buffer solution tank, the sample waste liquid tank and the waste liquid tank, and the high voltage electrodes are linked with the electrode points; a second layer is a bonding layer; a third layer is bonded on the first layer; and sampling holes are formed in the first layer; and a fourth layer is equipped with pneumatic micropumps. The dynamic PCR and CE functional integrated micro-fluidic chip of the microcavity has real-time performance and end point detection of one or more PCR segments.

Description

technical field [0001] The invention relates to a microfluidic chip integrating the functions of microchamber dynamic PCR and capillary electrophoresis CE, and belongs to the technical field of PCR and CE integration. Background technique [0002] Microfluidic chip (Microfludics) refers to a chemical or biological laboratory built on a chip of several square centimeters, which can rely on various electrical, magnetic, mechanical, chemical and other experimental operations under microscopic channels to achieve Design features. The goal is to integrate sample preparation, reaction, separation, detection, cultivation, sorting, etc. involved in the chemical and biological fields on a microchip, and it can be used multiple times. The final development direction is a micro-total analysis system. The biggest feature of the microfluidic chip is that the biochemical environment relies on the microfluidic environment produced by MEMS microfabrication, and runs through the entire syst...

AI Technical Summary

Problems solved by technology

Detectors are developed together with microfluidic chips, so they inherit the development of capillary electrophoresis chips in the early days of microfluidic chips. Optical detection methods including laser-induced fluorescence detection, chemiluminescence and ultraviolet absorption have always been the mainstream detection methods; electrochemical detection The method is cheap to detect, the device is simple, and it is very easy to integrate, and is suitable for some fields that do not require high sensitivity; mass spectrometry has high resolution, and has outstanding advantages in sensitivity and structural analysis capabilities, but the equipment is more expensive

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