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Preparation method of permanent middle cerebral artery occlusion model

An arterial occlusion, permanent technology, applied in the field of animal models, to achieve the effect of good repeatability, feasible scheme operation and simple operation

Inactive Publication Date: 2013-02-06
ZHONGSHAN HOSPITAL FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on a more clinical animal model of ischemic stroke

Method used

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  • Preparation method of permanent middle cerebral artery occlusion model
  • Preparation method of permanent middle cerebral artery occlusion model
  • Preparation method of permanent middle cerebral artery occlusion model

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Experimental method

[0025] 1. Experimental animals

[0026] SPF-grade male spontaneous hypertensive rats, weighing 220-270g, are provided by Shanghai Slack Laboratory Animal Co., Ltd. The rats are raised in an environment of 22-25℃, 12h circadian rhythm, free eating and drinking.

[0027] 2. Main instruments, microscopic instruments and tethered wires

[0028] Electric heating constant temperature incubator: developed by the Department of Physiology, Shanghai Medical College;

[0029] Non-invasive mouse tail artery measurement system (BPmesure 1.0): developed by the Department of Physiology, Shanghai Medical College;

[0030] MCAO thread plug: Beijing Shadong Biotechnology Co., Ltd.

[0031] 3. Wire bolt selection

[0032] The 2432 model (wire body diameter: 24nm; thread head diameter: 32nm) thread plug for rats from Beijing Shadong Biotechnology Co., Ltd. was selected.

[0033] 4. Preoperative blood pressure measurement

[0034] 24h before the operation, the rats of each group w...

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Abstract

The invention provides a preparation method of a permanent middle cerebral artery occlusion model. The preparation method comprises the following steps of: a, selecting a spontaneous hypertension rat; b, establishing the permanent middle cerebral artery occlusion model by utilizing a suture method. The preparation method has the advantages that the suture method is simpler in operation in comparison with sphenotresia; the scheme is practicable in operation, good in repeatability and high in success rate; and the clinical practice can be better fulfilled.

Description

technical field [0001] The invention relates to the field of animal models, in particular to a method for preparing a permanent middle cerebral artery occlusion model on the basis of spontaneously hypertensive rats. Background technique [0002] Stroke is a critical emergency with acute onset. Around the world, 16 million people suffer from stroke every year, and this number will triple by 2030. Ischemic stroke accounts for 60%-70% of the total number of stroke patients. Therefore, the basic and clinical research of ischemic stroke is always an important medical research topic. [0003] At present, there are many animal experiments on the drug treatment of ischemic stroke, but once the effective drugs are transferred to the clinic, few significant curative effects can be observed. The reason may be related to the fact that most of the previous animal experiments used rats with normal blood pressure. It is well known that hypertension is the most important risk factor for...

Claims

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Application Information

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IPC IPC(8): A61D1/00A61K49/00
Inventor 范薇
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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