Preparation method of redox probe for marking CRP (C-Reactive Protein) impedance immunosensor

An immunosensor and label-free technology, which is applied in the direction of material resistance, material electrochemical variables, and material inspection products, can solve the problems of being unable to be used as electrochemical immunosensor probes, achieve low detection limits, good reproducibility, and preparation The effect of simple method

Inactive Publication Date: 2013-02-06
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
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Problems solved by technology

Common Redox Probe K4Fe(CN) 6 Often unavailab

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0018] (1) Synthesis of terephthalaldehyde-condensed o-aminothiophenol Schiff base:

[0019] Under stirring conditions, slowly add 40 mL of absolute ethanol solution containing 10 mmol of aminothiophenol dropwise to 20 mL of absolute ethanol solution dissolved with 10 mmol of terephthalaldehyde, and heat in a water bath at 60°C for 4 hours to obtain a bright yellow precipitate. Recrystallize with ethanol and dry in vacuum to get terephthalaldehyde-condensed o-aminothiophenol Schiff base.

[0020] (2) Synthesis of terephthalaldehyde-condensed o-aminothiophenol Schiff base nickel complex:

[0021] Prepare a mixed solution of absolute ethanol and N,N'-dimethylformamide (DMF) at a volume ratio of 2:1, and dissolve 1 mmol of the terephthalic acid obtained in step (1) into 5 mL under stirring conditions. Slowly add 8 mL of N,N'-dimethylformamide solution containing 2 mmol of nickel acetate dropwise to the above mixed solution of formaldehyde-condensed o-aminothiophenol Schiff bas...

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Abstract

The invention discloses a preparation method of a redox probe for marking a CRP (C-Reactive Protein) impedance immunosensor. The redox probe is made of a terephthalaldehyde o-aminophenol thiophenol Schiff base nickel complex and nanogold sol. The nanogold sol in the redox probe and the sulfydryl Schiff base nickel complex have relatively strong interaction, so that stronger impedance detection signals can be provided for an unmarked CRP impedance immunosensor, and the detection performance of the immunosensor is effectively improved. The redox probe is simple to prepare and has the advantages of low detection limit, good stability, good reproducibility and the like when being used for marking the CRP impedance immunosensor to detect the CRP content.

Description

technical field [0001] The invention relates to a preparation method of a redox probe used for an unmarked CRP (C-reactive protein) impedance type immunosensor. Background technique [0002] CRP is an acute phase glycoprotein produced by liver cells in the human body and can bind to the cell wall of pneumococcus. The normal reference value of the content in the human body is usually ≤10mg / L, and the content is extremely small. However, when the human body is invaded by foreign bacteria or viruses, causing acute inflammation, local tissue damage or ischemia, and malignant tumors, the liver will rapidly secrete and produce a large amount of CRP under the regulation of interleukin-6. Therefore, in clinical practice, the content of CRP in the human body is often used as an important physiological indicator for judging tumor progression, acute inflammation, and acute infectious diseases. Therefore, how to find a fast, effective, easy-to-operate and low-cost detection method has ...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/06G01N33/68
Inventor 刘峥袁帅刘进
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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