Super-resolution microscopic imaging method and device based on micro waveguide

Abstract

The invention discloses a super-resolution microscopic imaging method based on micro waveguide. The super-resolution microscopic imaging method comprises following steps of: (1) leading continuous spectrum laser coupling into the micro waveguide, placing the micro waveguide on the surface of a sample with a sub-wavelength detail, wherein the surface detail has an effect of modulating light transmitted in the micro waveguide to form scattered light; (2) collecting scattered light signals, and imaging to acquire corresponding images; (3) performing spectral analysis on images in the step (2) to acquire a spectrum distribution diagram; and (4) performing frequency shift reconstruction on the spectrum distribution diagram, and inverting to acquire a sample surface image capable of resolving subwavelength. The invention also discloses a super-resolution microscopic imaging device based on the micro waveguide. The super-resolution microscopic imaging method and device have the advantages of high fineness of resolution and high functional expansibility and can be effectively applied to the super-resolution microscopic imaging of the sub-wavelength detail on the surface of the sample.

Description

technical field [0001] The invention relates to the field of microscopic observation and measurement, in particular to a microwave-guided super-resolution microscopic imaging method and device. Background technique [0002] Nanotechnology and biotechnology are the fastest-growing and hottest scientific fields in the 21st century. Nanotechnology has a wide range of applications, including imaging, measurement, processing, manipulation, etc. within the scale of 1-100nm. Many important organisms such as glucose, antibodies, viruses, etc. are in this scale range, and the need to study these tiny objects has driven the development of high-resolution microscopy. In turn, the development of super-resolution microscopy has also promoted the progress of the entire life sciences. A major advantage of light microscopy over other microscopy techniques is the ability to study living cells in their natural state. [0003] Since the world's first optical microscope came out, improving t...

AI Technical Summary

Problems solved by technology

The electron microscopic imaging technology developed in the 1930s and various non-optical probe scanning microscopic imaging technologies that emerged in the early 1980s have nanometer or even higher resolution capabilities, but they exist to varying degrees. The complex structure of the system, the harsh requirements of the imaging and detection environment, and the cumbersome sample processing are difficult, especially the important optical information of the sample (such as reflectivity, refractive index, polarization state, and spectrum, etc.) cannot be obtained, so it cannot completely replace the optical microscopic imaging. status

However, both existing technologies have certain defects: although the former uses wide-field illumination, it is difficult to compress its resolution below 100nm; the latter is based on fluorescence microscopy and cannot be used on non-fluorescent samples , so the scope of use is limited

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