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Method for detecting phenol compounds in water body environment through enzyme electrochemical biosensor

A phenolic compound, electrochemical technology, applied in the field of biosensing, to improve sensitivity, prevent unfolding inactivation, improve response speed and sensitivity

Active Publication Date: 2014-09-10
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] The technical problem to be solved in the present invention is to improve the long-term stability of the tyrosinase electrochemical sensor, and provide a detection method based on the tyrosinase electrochemical sensor applied to the screening of phenolic pollutants in water bodies. Detection of phenolic pollutants

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  • Method for detecting phenol compounds in water body environment through enzyme electrochemical biosensor
  • Method for detecting phenol compounds in water body environment through enzyme electrochemical biosensor
  • Method for detecting phenol compounds in water body environment through enzyme electrochemical biosensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1. Tyrosinase electrochemical biosensor assembly

[0037] Glassy carbon electrode assembly steps:

[0038] 1) A glassy carbon electrode with a diameter of 0.3 cm was polished with Al2O3 powders with particle sizes of 1 μm, 0.3 μm, and 0.05 μm, and then ultrasonically cleaned in deionized water for 3 times, each time for 2 minutes. Put in 2mmol l L -1 The cyclic voltammetry curve was scanned between -0.1-0.6V in the potassium ferricyanide solution. The redox peak potential difference of the curve is less than 75mV, indicating that the potassium ferricyanide reaction on the surface of the glassy carbon electrode is a completely reversible reaction, and there is no impurity on the electrode surface.

[0039] 2) Construction of supramolecular self-assembly of tyrosinase mesoporous carbon materials. Monodisperse silica gel spheres prepared by the sol-gel method were used as hard templates, polystyrene was used as carbon source, and ordered mesoporous carbon with a...

Embodiment 2

[0043] Example 2. Standard solutions for the detection of catechol, phenol and nonylphenol by tyrosinase electrochemical biosensor

[0044] Dissolve catechol, phenol and nonylphenol in acetonitrile solution to make 1mmol L -1 standard solution.

[0045] Use the Shanghai Chenhua Electrochemical Workstation (Chi 440B) to scan the curve of current and time under the condition of -0.1V constant potential, and add to 8mL 50mmol L at intervals of 40s -1 Add 4μL 1mmol L dropwise to the phosphate buffer solution -1 The catechol standard solution was used to obtain the correlation curve between the current signal and time. Put the tyrosinase electrochemical sensor in the blank detection solution (50mmol L -1 The response signal of the scanning record in the phosphate buffer solution) is recorded as I a , the response signal obtained by adding the catechol standard solution into the blank detection solution is recorded as I b ; Taking the catechol concentration (c) as the abscissa,...

Embodiment 3

[0047] Example 3. Detection of Potential Interfering Substances of Tyrosinase Electrochemical Biosensor

[0048] Dissolve the following compounds in acetonitrile to make 100mmol L -1 tyrosinase electrochemical biosensor to record changes in electrical signals; the detection compounds are: tartaric acid, sodium citrate, sodium oxalate, urea, ethyl acetate, diethyl carbonate, and glucose. The detection process of the above compounds is the same as that of catechol.

[0049]Compounds such as ethyl acetate are not specific substrates of tyrosinase, and the current signal change intensity (w) produced is less than 0.13 microamperes (three times the signal-to-noise ratio), that is, they are not detected. As the reaction substrate of tyrosinase, catechol and other phenolic compounds cause the change of the electrochemical response signal of the tyrosinase electrochemical biosensor to be much greater than 0.13 microamperes, which is obviously detected; therefore, tyrosinase can be us...

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Abstract

The present invention relates to a method for detecting phenol compounds in a water body environment through an enzyme electrochemical biosensor. The biological sensor detection apparatus comprises a working electrode, an auxiliary electrode, a reference electrode, a detection cell and an electrochemical workstation. The specific operation steps comprise: adding a hydrophilic ionic liquid (such as 1-butyl-3-methylimidazolalanine and BMIM [Ala]) to a mesoporous carbon solution, and shaking for half an hour to obtain a novel mesoporous carbon and hydrophilic ionic liquid composite material; adding tyrosinase to the mesoporous carbon and hydrophilic ionic liquid composite material solution, shaking for one hour, and adding the obtained solution to the surface of a working electrode in a dropwise manner to obtain a tyrosinase immobilized working electrode; and inserting the tyrosinase immobilized working electrode, the auxiliary electrode and the reference electrode into a detection cell filled with a phosphate buffer solution, and adding a single standard substance of a phenol compound under a stirring condition to produce a corresponding current response signal.

Description

technical field [0001] The invention belongs to the field of biosensing, and in particular relates to a method for detecting phenolic compounds in a water body environment by an electrochemical sensor using a novel amino acid ionic liquid-modified mesoporous carbon composite material immobilized with tyrosinase. Background technique [0002] Phenolic compounds are a class of organic pollutants that widely exist in the environment, mainly from coking, pharmaceutical, chemical and other industrial emissions and pesticide degradation. Phenolic compounds in drinking water sources can produce chlorophenols, which are more toxic and have "three-effect" effects in the water treatment process of chlorination and disinfection, thus threatening the safety of drinking water. [0003] As people pay more and more attention to water environment safety and health, it is urgent to quickly judge the safety status of surface water and drinking water sources. As a class of important organic p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
Inventor 吴立冬卢宪波苏凡陈吉平
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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