Method for finding E2-E3 specifically mediating target protein ubiquitination reaction based on known E1
A target protein, specific technology, applied in the field of biochemistry and molecular biology, can solve the problems of terror and unclear ubiquitin modification mechanism
Inactive Publication Date: 2013-02-20
2 Cites 5 Cited by
AI-Extracted Technical Summary
Problems solved by technology
The invention relates to the fields of biochemistry and molecular biology, and specifically relates to a method forfinding an ubiquitin ligase E3 specifically identifying a target protein and a E2/E3 system for regulating target protein ubiquitination reaction from a number of E3 of human cells. According to the invention, an in-vitro ubiquitination analysis system is established; a known ubiquitin activating enzyme E1 and HelaS100 fraction are adopted as E3 sources, and a ligase E2 mediating the target protein ubiquitination reaction is screened; and a multiple chromatographic column combination is combined with mass spectrometric analysis, such that the ubiquitin ligase E3 specifically identifying the target protein and the E2/E3 system regulating target protein ubiquitination modification are searched from human cells. With the method, the target-protein-identifying substrate recognition factor which is ubiquitin ligase E3 can be conveniently and accurately found, and a E2/E3 system mediating target protein ubiquitination modification can be established.
Microbiological testing/measurementBiological testing
Chromatographic columnUbiquitin +9
- Experimental program(6)
Description & Claims & Application Information
We can also present the details of the Description, Claims and Application information to help users get a comprehensive understanding of the technical details of the patent, such as background art, summary of invention, brief description of drawings, description of embodiments, and other original content. On the other hand, users can also determine the specific scope of protection of the technology through the list of claims; as well as understand the changes in the life cycle of the technology with the presentation of the patent timeline. Login to view more.